포도주스 침지 제조 흑삼의 Ginsenoside Rg₃ 함량 변화와 Acetylcholinesterase 억제효과

• Published in: Journal of ginseng research Vol. 33; no. 4; pp. 349 - 354
• Main Authors: 이미라(Mi-Ra Lee), 윤범식(Beom-Sik Yun), 손백신(Bai-Shen Sun), 류뢰(Lei Liu), 장동량(Dong-Liang Zhang), 왕춘년(Chun-Yan Wang), 왕젠(Zhen Wang), 이선영(Sun-young Ly), 모은경(Eun-Kyung Mo), 성창근(Chang-keun Sung)
• Format: Journal Article
• Language: Korean
• Published: 고려인삼학회 01.12.2009
• Subjects: 기타의약학; ginsenoside Rg; acetylcholinesterase; black ginseng; grape juice; HPLC
• ISSN: 1226-8453; 2093-4947

This study was conducted to develop a new method for enhancing ginsenoside Rg3, which is abundant in black ginseng. The cognition-enhancing effect of black ginseng extract was investigated via the assay of acetylcholinesterase (AChE) activity. Black ginseng I was prepared through the traditional method (by steaming and drying nine times repetitions). Black ginseng II, on the other hand, was prepared by steaming the ginseng three times at 120oC for 30 min after soaking it in grape juice for 24 h. The ginsenosides of white, red, and black ginseng I, and II were investigated using the HPLC method, respectively. In black ginseng II, the ginsenoside Rg3 contents, which cannot be found in white ginseng, amounted to 10.91 mg/g, approximately 18 times more than that in red ginseng. In the in-vivo study, black ginseng extract (200 mg/kg, p.o.) inhibited the AChE activity after 24 h by a single administration in the brain. Thus, the new manufacturing method for black ginseng was found to more effective in the conversion of ginsenoside Rg3 compared to the traditional method. Black ginseng may also have the effect of preventing the cognitive impairment induced by cholinergic dysfunction. KCI Citation Count: 10