柴梗半夏湯이 LPS로 유발된 급성 폐손상에 대한 영향

• Published in: 동의생리병리학회지 Vol. 23; no. 6; pp. 1349 - 1357
• Main Authors: 김기태(Ki Tae Kim), 고흥(Heung Ko)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.12.2009
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation , control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/㎏, 6.7 g/㎏) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histiology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-α, IL-1β, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT(100 ug/㎖, 500 ug/㎖, 1000 ug/㎖) on the release of RANTES, TARC induced by TNF-α and IL-4 in human aveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-α, IL-1β and IL-6 according to concentrations. In vitro, 500 ug/㎖, 1000 ug/㎖ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI. This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation , control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/㎏, 6.7 g/㎏) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histiology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-α, IL-1β, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT(100 ug/㎖, 500 ug/㎖, 1000 ug/㎖) on the release of RANTES, TARC induced by TNF-α and IL-4 in human aveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-α, IL-1β and IL-6 according to concentrations. In vitro, 500 ug/㎖, 1000 ug/㎖ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI. KCI Citation Count: 3