생쥐의 췌장도세포추출을 위한 콜라겐분해효소 용액 주입 시 쓸개와 총담관 경로의 비교

• Published in: Annals of surgical treatment and research Vol. 73; no. 1; pp. 8 - 12
• Main Author: 박종권(Jong Kwon Park)
• Format: Journal Article
• Language: Korean
• Published: 대한외과학회 01.07.2007
• Subjects: 일반외과학; Common bile duct; 총담관; 쓸개; Pancreatic islet; Gall bladder; 췌장도세포
• ISSN: 2288-6575; 2288-6796

Purpose: A high yield of viable pancreatic islets is an essential prerequisite for the study of pancreatic islet transplantation. The purpose of this study is to compare the yield between intra-gall bladder (intra-GB) and intra-common bile duct (intra-CBD) injection of collagenase solution for isolation of mouse pancreatic islets. Methods: The mice were divided into two groups, the intra-GB and intra-CBD groups, and each group included twelve mice, respectively. Collagenase solution was injected via the gallbladder in the intra-GB group mice, while this was done via the common bile duct in the intra-CBD group. After removal and digestion of the mouse pancreases, the pancreatic islets were isolated by Ficoll density gradient centrifugation and hand picking. Results: The intra-GB group yielded 121.67±39.86 IEQs, and the intra-CBD group reveled 168.17±29.23 IEQs. There was a statistically significant difference in islet yield between the two groups (P=0.005, Mann-Whitney Test). The purities of the isolated islets were 86.42±3.99% for the intra-GB group and 87.17±4.47% for the intra-CBD group, and there was no difference between the two groups (P=0.755, Mann- Whitney Test). Conclusion: Both the intra-GB and intra-CBD groups yielded an average of ＞120 IEQs. However, the intra-CBD group revealed a higher yield than the intra-GB group for isolating mouse pancreatic islets. (J Korean Surg Soc 2007;73:8-12) KCI Citation Count: 0