DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells
The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (...
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| Published in | Molecules and cells Vol. 41; no. 12; pp. 1045 - 1051 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Korean Society for Molecular and Cellular Biology
31.12.2018
한국분자세포생물학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1016-8478 0219-1032 |
| DOI | 10.14348/molcells.2018.0129 |
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| Abstract | The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased 'slow and long-lived' subpopulations, but decreased 'fast and short-lived' subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells. |
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| AbstractList | The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased 'slow and long-lived' subpopulations, but decreased 'fast and short-lived' subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells. The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased ‘slow and long-lived’ subpopulations, but decreased ‘fast and short-lived’ subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells. KCI Citation Count: 5 |
| Author | Back, Sung Hoon Jang, Soo Hwa Park, Jeong Woo Han, In-Seob Dang, Thao |
| AuthorAffiliation | 1 School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea |
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| Keywords | DRG2 tau microtubule dynamics |
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| SubjectTerms | Cell Proliferation - physiology Gene Knockdown Techniques GTP-Binding Proteins - deficiency HeLa Cells Humans Microtubules - metabolism Phosphorylation tau Proteins - metabolism Transfection 생물학 |
| Title | DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells |
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