Role of α-Actinin 2 in Cytoadherence and Cytotoxicity of Trichomonas vaginalis
is a pathogen that triggers severe immune responses in hosts. α-actinin 2 (Tvα-actinin 2) has been used to diagnose trichomoniasis. Tvα-actinin 2 was dissected into three parts; the N-terminal, central, and C-terminal portions of the protein (#1, #2, and #3, respectively). Western blot of these Tvα-...
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Published in | Journal of microbiology and biotechnology Vol. 27; no. 10; pp. 1844 - 1854 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
한국미생물·생명공학회
01.10.2017
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Subjects | |
Online Access | Get full text |
ISSN | 1017-7825 1738-8872 |
DOI | 10.4014/jmb.1706.06050 |
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Summary: | is a pathogen that triggers severe immune responses in hosts.
α-actinin 2 (Tvα-actinin 2) has been used to diagnose trichomoniasis. Tvα-actinin 2 was dissected into three parts; the N-terminal, central, and C-terminal portions of the protein (#1, #2, and #3, respectively). Western blot of these Tvα-actinin 2 proteins with pooled patients' sera indicated that #2 and #3, but not #1, reacted with those sera. Immunofluorescence assays of two different forms of
(trophozoites and amoeboid forms), using anti-Tvα-actinin 2 antibodies, showed localization of Tvα-actinin 2 close to the plasma membranes of the amoeboid form. Fractionation experiments indicated the presence of Tvα-actinin 2 in cytoplasmic, membrane, and secreted proteins of
. Binding of fluorescence-labeled
to vaginal epithelial cells and prostate cells was decreased in the antibody blocking experiment using anti-Tvα-actinin 2 antibodies. Pretreatment of
with anti-rTvα-actinin 2 antibodies also resulted in reduction in its cytotoxicity. Flow cytometry, ligand-binding immunoblotting assay, and observation by fluorescence microscopy were used to detect the binding of recombinant Tvα-actinin 2 to human epithelial cell lines. Specifically, the truncated N-terminal portion of Tvα-actinin 2, Tvα-actinin 2 #1, was shown to bind directly to vaginal epithelial cells. These data suggest that α-actinin 2 is one of the virulence factors responsible for the pathogenesis of
by serving as an adhesin to the host cells. |
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ISSN: | 1017-7825 1738-8872 |
DOI: | 10.4014/jmb.1706.06050 |