커피 유전자원의 항산화 활성과 하이드록시신남산 함량 비교

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi pp. 659 - 667
• Main Authors: 고승미, 김기창, 강해주
• Format: Journal Article
• Language: Korean
• Published: 한국식품영양과학회 31.08.2025
• Subjects: 식품과학
• ISSN: 1226-3311; 2288-5978
• DOI: 10.3746/jkfn.2025.54.8.659

본 연구는 코스타리카에서 재배된 Coffea arabica L. 유전자원 22종의 항산화능과 hydroxycinnamic acid 함량을 비교하고 커피 신품종 육성을 위한 유망 소재 및 기초 정보를 제공하고자 수행하였다. 미디움 로스팅한 아라비카종의 총 폴리페놀 함량은 5.14~12.37 mg GAE/g 범위에 있었으며 평균 함량은 7.50±2.05 mg GAE/g으로 나타났고 DPPH 및 ABTS 라디칼 소거능은 각각 IC50 19.86~35.57, 26.79 ~40.35 μg/mL였다. Hydroxycinnamic acid 함량을 분석한 결과, 평균 23.33±3.76 mg/g이었으며, 5-O-caffeoylquinic acid는 평균 8.28±1.43 mg/g으로 전체 hydroxycinnamic acid 중 약 35.5%를 차지하는 것으로 나타났다. PCA 결과 총 폴리페놀과 caffeoylquinic acids 화합물 함량이 높을수록 항산화능이 우수한 경향을 보였으며, 특히 T.04517, T.04584, T.21315가 우수 유전자원으로 확인되었다. 본 연구 결과는 향후 항산화능이 뛰어난 유전자원 선별 및 육종 연구의 기초자료로 활용될 수 있을 것으로 기대된다. As a major dietary source of polyphenol and antioxidant compounds, coffee plays a key role in the human diet. In this study, 22 germplasms of Coffea arabica L. cultivated in Costa Rica were comprehensively characterized for hydroxycinnamic acid composition and antioxidant activity, with the objective of providing foundational data for the development of novel cultivars. The mean total phenolic content was 7.50±2.07 mg gallic acid equivalent/g. Antioxidant activity, as determined by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays, yielded half maximal inhibitory concentration (IC50) values ranging from 26.79 to 40.35 µg/mL and 17.69 to 35.57 µg/mL, respectively. The total hydroxycinnamic acid concentration ranged from 16.13 to 33.24 mg/g, with 5-O-caffeoylquinic acid identified as the predominant component at 8.32±1.45 mg/g, representing 35.5% of the total hydroxycinnamic acid content. Principal component analysis demonstrated a strong positive association between elevated total phenolic contents, caffeoylquinic acids levels, and antioxidant activity. In particular, T.04517, T.04584, and T.21315 were distinguished as high-activity germplasms. Collectively, these findings furnish critical baseline information to guide the selection and breeding of coffee germplasms with enhanced antioxidant potential. KCI Citation Count: 0