Veronica incana 추출물의 생물학적 활성 평가

• Published in: 大韓本草學會誌 Vol. 39; no. 3; pp. 57 - 67
• Main Authors: 신미래, Mi-rae Shin, 윤미영, Mi Yeong Yoon, 김민주, Min Ju Kim, 정일하, Il-ha Jeong, 안희연, Hui Yeon An, 정지원, Ji-won Jung, 노성수, Seong-soo Roh
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.05.2024
• Subjects: anti-inflammatory; anti-obesity; antioxidant; Veronica incana; 한의학; anti-inflammatory; Veronica incana; antioxidant; anti-obesity
• ISSN: 1229-1765; 2288-7199
• DOI: 10.6116/kjh.2024.39.3.57

Objectives : The aim of this study is to evaluate the potential biological activity of Veronica incana extracts (VIE) through in vitro, ex vivo, and in vivo experiments. Methods : In vitro, we conducted analyses on the total polyphenol (TP) and total flavonoid (TF) levels, alongside DPPH and ABTS radical scavenging activities. Ex vivo evaluations on adipose tissue measured glycerol release as a marker of lipolysis. In LPS-induced RAW 264.7 cells, we quantified nitric oxide (NO) production. Following H2O2 induction in U2OS cells, we performed mitochondrial assays such as MitoSox and MitoTracker. Moreover, Bodipy assays were conducted in 3T3-L1 cells. In vivo, we performed anti-osteoarthritis effect of VIE against monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Results : The results presented encompass a myriad of models, from cell culture to animal experiments as well as ex vivo studies. VIE demonstrated high TP and TF contents, potent DPPH and ABTS scavenging activities, and regulated glycerol release. Moreover, the inhibition of NO production in LPS-induced inflammation was notably confirmed and the reduction of lipid droplets was distinctly shown. Furthermore, in H 2 O 2 -induced U2OS cells, MitoSox was effectively reduced while MitoTracker noticeably increased. In vivo assays confirmed a significant increase in hindpaw weight distribution (HWD) decreased by MIA after VIE treatment. Additionally, VIE inhibited serum inflammatory cytokines (TNF-α, IL-6, and IL-1β) and MDA levels in joint tissue. Conclusion : In conclusion, Veronica incana exhibited various pharmacological effects including antioxidant, anti-obesity, and anti-inflammatory properties.