기계적 자극이 다층 구조의 나노파이버 지지체의 평활근 세포에 미치는 영향

• Published in: Journal of biomedical engineering research Vol. 29; no. 1; pp. 52 - 58
• Main Authors: 신지원, 김동화, 허수진, 김수향, 김영직, 신정욱, Shin, Ji-Won, Kim, Dong-Hwa, Heo, Su-Jin, Kim, Su-Hyang, Kim, Young-Jick, Shin, Jung-Woog
• Format: Journal Article
• Language: Korean
• Published: 대한의용생체공학회 01.02.2008
• Subjects: 의공학; vascular tissue engineering; smooth muscle cell; cell alignment; cyclic stretch; resting period
• ISSN: 1229-0807; 2288-9396

The object of this study is to investigate the effects of intermittent cyclic stretching on the smooth muscle cells (SMCs) seeded onto aligned multi-layered fibrous scaffold. To make multi-layered fibrous scaffold, polyurethane (PU) and poly(ethylene oxide) (PEO) were electrospun alternatively, then were immersed into distilled water to extract PEO. Various types of scaffolds were fabricated depending on fiber directions, i.e., aligned or randomly oriented. The direction of stretching was either parallel or vertical to the fiber direction for the aligned scaffolds. The stretching was also applied to the randomly aligned scaffolds. The duration of stretching was 2 min with 15 min resting period. During the stretching, the maximum and minimum strain was adjusted to be 10 and 7%, respectively with the frequency of 1 Hz. The bioactivities of cells on the scaffolds were assessed by quantifying DNA, collagen, and glycosaminoglycan (GAG) levels. And the cell morphology was observed by staining F-actin. SMCs under parallel stretching to the fiber direction responded more positively than those in other conditions. From the results, we could explain the morphological effect of a substrate on cellular activities. In addition the synergistic effects of substrate and mechanical stimuli effects were confirmed.