다배란 처리에 따른 한우 체내 수정란 생산 효율과 수정란이식

• Published in: Journal of animal reproduction & biotechnology (Online) pp. 141 - 144
• Main Authors: 조상래(농촌진흥청 축산기술연구소, 최선호(농촌진흥청 축산기술연구소, 최창용(농촌진흥청 축산기술연구소, 손준규(농촌진흥청 축산기술연구소, 최수호, 김영주, 이풍연(농촌진흥청 축산기술연구소, 연성흠, 김현종(농촌진흥청 축산기술연구소, 손동수
• Format: Journal Article
• Language: Korean
• Published: 사단법인 한국동물생명공학회 30.09.2010
• Subjects: 축산학
• ISSN: 2671-4639; 2671-4663

In vivo embryo produced from Hanwoo donor cows were collected and transferred to Hanwoo recipients. Cows, at random stages of the estrous cycle, received Progesterone Releasing Intravaginal Device (CIDR-plus, InterAg, New Zealand) together with injection of 1 mg estradiol benzoate and 50 mg progesterone, and gonadotropin treatment began 4 day later. For superovulation, a total of 28 mg FSH was intramuscularly injected twice a day in the way of decresing doses 4 day (5, 5, 4, 4, 3, 3, 2 and 2 mg). Twenty one Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered 7 days after the second insemination by flushing the uterus with Embryo Collection Medium. The results obtained were as follows: The rates of transferable embryos were 50.3%, and 78 fresh embryos at morulae and blastocysts stage were transferred into Hanwoo recipients on day 7 of estrus cycle. The pregnancy rates were first embryo transfer 55.6%, 2nd 62.9% and 3rd 57.9%, respectively. In conclusion, These results suggest that CIDR-based superovulation protocol may be effectively used for production of superior Hanwoo embryos. Also, since it seems the condition of recipient cows greatly affect pregnancy rate, it is very important to evaluate recipient for effective cattle production. KCI Citation Count: 4