간경변증 환자의 말초혈액단핵구에서 중합효소연쇄반응법을 이용한 Cytomegalovirus DNA 의 검출

• Published in: The Korean journal of medicine Vol. 49; no. 6; pp. 742 - 752
• Main Authors: 문종호, Jong Ho Moon, 유창범, Chang Beom Ryu, 박철호, Cheol Ho Park, 류권호, Kwon Ho Ryu, 박찬욱, Chan Wook Park, 이준성, Joon Sung Lee, 이문성, Moon Sung Lee, 조성원, Sung Won Cho, 황승덕, Seung Deok Hwang, 심찬섭, Chan Sup Shim, 이희발, Hi Bahl Lee
• Format: Journal Article
• Language: Korean
• Published: 대한내과학회 01.12.1995
• Subjects: Cytomegalovirus; Liver cirrhosis; Polymerase chain reaction; Shell vial culture
• ISSN: 1738-9364

Objectives: Human cytomegalovirus(CMV) infections are common and usually asymptomatic, but fatal infections occur frequently in immunocompromised patients. In Korea, CMV infection is common and patients with liver cirrhosis are frequently in a condition of immunosuppression, may predispose to the frequent occurrence of CMV infections. The purpose of this study was to investigate the prevalence of CMV infection in patients with liver cirrhosis and to evaluate the relationship between the status of C3:IV infection and liver function. Methods, The subjects of this study were 36patients with liver cirrhosis, 4patients with non A, non B, non C(NANRNC) hepatitis, and 13normal controls. IgG and IgKI antibodies to CMV were measured using a microparticle enzyme immunoassay(MEIA). Specimens of urine were cultured for CMV using shell vial culture method. Peripheral blood mononuclear cells(PBMC) were investigated for the presence of CMV-DNA using PCR. Rusults: In CMU antibody test, IgM and IgG antibodies were detected in 7(19.4%) of 36patients with liver cirrhosis, IgG antibody was detected in the other 29patients and all control subjects. In shell vial culture, CMV was cultured in 1(2.8%) of 36patients with liver cirrhosis(IgM and IgG positive). CMV DNA was detected by PCR in 19(52.8%) of 36patients with liver cirrhosis, but 1(7.7%) of 13 control subjects (p<0.05). CMV DNA was detected in 6(85.7%) of 7cirrhotic patients with IgM and IgG antibodies positive, 13(44.8%) of 29cirrhotic patients with IgG antibody positive. CMV DNA positive cirrhotic patients show 5(26.3%) in Child A, 6(31.6%) in Child H, 8(42.1%) in Child C. CMV DNA negative cirrhotic patients show 4(23.5%) in Child A, 12(70.6%) in Child B, 1(5.9%) in Child C(p<0.05). CMV DY:A positive cirrhotic patients showed the tendency of more frequent detection of HBsAg and HBeAg than CMU DNA negative cirrhotic patients(p<0.05). Conclusion: These results suggest that CMV infection is common in cirrhotic patients and the CMV DNA positive patients with liver cirrhosis have more impairment of liver function than the CMV DNA negative patients with liver cirrhosis.