자소엽(Perilla frutescens Britton var. acuta Kudo) 조다당의 멜라닌 생성 저해 및 미백효과

• Published in: Han'guk Sikp'um Kwahakhoe Chi = Korean Journal of Food Science and Technology Vol. 51; no. 1; pp. 58 - 63
• Main Authors: 조은지(Eun-Ji Cho), 변의홍(Eui-Hong Byun)
• Format: Journal Article
• Language: Korean
• Published: Seoul 한국식품과학회 01.02.2019; Korean Society of Food Science & Technology
• Subjects: Cosmetics; Functional foods & nutraceuticals; Melanin; Melanoma; Microphthalmia; Perilla frutescens; Polysaccharides; Proteins; Synthesis; Tyrosinase; Western blotting; 식품과학; tyrosinase related protein-2; microphthalmia-associated transcription factor; tyrosinase; Perilla frutescens Britton var. acuta Kudo; tyrosinase related protein-1; acuta Kudo; Perilla frutescens Britton var
• ISSN: 0367-6293; 2383-9635
• DOI: 10.9721/KJFST.2019.51.1.58

In this study, the inhibitory effects of crude polysaccharide fractions separated from Perilla frutescens Britton var. acuta Kudo (PCP) on melanin synthesis and tyrosinase activity were observed. B16F10 melanoma cells were treated with 125 and 250μg/mL of PCP for 24 hours. Using these optimal concentrations, inhibition of melanin synthesis inhibition was measured, and PCP treatment significantly reduced melanin synthesis induced by 3-isobutyl-1-methylxanthine (IBMX). In addition, western blotting analysis on B16F10 melanoma cells showed that PCP inhibited tyrosinase, microphthalmia-associated transcriptipn factor, tyrosinase related protein-1, and tyrosinase related protein-2 expression. Therefore, these results indicate that PCP may have potential inhibitory activity against melanin synthesis and may be a natural ingredient useful for the development of whitening materials in cosmetics and functional foods.