Coxsackievirus B3의 VP2 부분에 대한 Peptide 항체 개발

• Published in: Journal of bacteriology and virology Vol. 36; no. 2; pp. 109 - 117
• Main Authors: 정수영, Soo Young Chung, 조영주, Young Joo Cho, 김연정, Yeun Jung Kim, 김대선, Dae Sun Kim, 이희란, Heui Ran Lee, 남재환, Jae Hwan Nam
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 2006; 대한미생물학회
• Subjects: Coxsackievirus B3; Peptide antibody; VP2; 생물학; Peptide antibody; VP2; Coxsackievirus B3
• ISSN: 1598-2467; 2093-0429

Coxsackievirus B3 (CVB3) is the nonenveloped virus containing a single-stranded positive-sense RNA as a genome. CVB3 infection can induce acute myocarditis and dilated cardiomypathy. CVB3 of icosahedral symmetry has four capsid proteins called VP1, VP2, VP3, and VP4. Although VP1 is a major antigenic determinant, VP2 is also an important protein for viral physiology, such as maturation cleavage and attenuation. However, VP2 study has been hampered, partly because VP2 antibody is not available. In this study, we developed peptide-based polyclonal VP2 antibody and analyzed its potency by Western blotting analysis and immunofluorescent assay. Purified B3-1 antibody (VP2 peptide antibody developed in here) showed the sensitivity and specificity, similar to VP1 monoclonal antibody which is commercially available. Moreover, this peptide antibody may be useful for double-staining with other antibodies derived from mouse. Therefore, the VP2 antibody may allow us to study CVB assembly and understand VP2 function in depth.