術中迅速フローサイトメトリーシステムの開発

DNA ploidy analysis is one of the two principal applications of flow cytometry, along with cell surface antigen analysis. DNA ploidy analysis is positive in numerous tumors, but no clear conclusion can be obtained in some tumors. This may be attributed in part to measuring inconsistency and interpre...

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Published inサイトメトリーリサーチ Vol. 27; no. 2; pp. 9 - 15
Main Authors 塩山 高広, 鈴木 あかね, 野村 健一, Kubo Hirotsugu, Kobayashi Naoki, 新田 雅之, 丸山 隆志, 村上 知之, Komori Takashi, 村垣 善浩
Format Journal Article
LanguageJapanese
Published 日本サイトメトリー学会 2017
Japan Cytometry Society
Subjects
DNA aneuploidy
DNA ploidy analysis automatic measurement system
Flow cytometry
glioma
Online AccessGet full text
ISSN0916-6920
2424-0664
DOI10.18947/cytometryresearch.27.2_9

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Abstract DNA ploidy analysis is one of the two principal applications of flow cytometry, along with cell surface antigen analysis. DNA ploidy analysis is positive in numerous tumors, but no clear conclusion can be obtained in some tumors. This may be attributed in part to measuring inconsistency and interpretation bias in the analysis. As the measurement method is complex for DNA ploidy analysis of solid tumors, it is difficult to conduct the analysis as a routine clinical procedure. Hence, we have developed the system named “High DNA Content Measuring Flow Cytometer” with a tissue pretreatment function, and a freeze-dried reagent kit for cell staining, in order to perform DNA ploidy analysis easily and reproducibly in non-specialized laboratories. Biopsy specimens from brain tumors of 87 glioma patients who underwent open surgery were analyzed to evaluate the utility of our system. We were able to automatically analyze the biopsy specimens with the system within ten minutes. We compared the results of the same pretreated samples between our system and the conventional method using a common flow cytometer, confirming that the malignancy index was well correlated between them (R=0.975). These results demonstrated that our fully automated DNA ploidy analyzer is useful for rapid determination of glioma presence in surgical biopsy specimens.
AbstractList DNA ploidy analysis is one of the two principal applications of flow cytometry, along with cell surface antigen analysis. DNA ploidy analysis is positive in numerous tumors, but no clear conclusion can be obtained in some tumors. This may be attributed in part to measuring inconsistency and interpretation bias in the analysis. As the measurement method is complex for DNA ploidy analysis of solid tumors, it is difficult to conduct the analysis as a routine clinical procedure. Hence, we have developed the system named “High DNA Content Measuring Flow Cytometer” with a tissue pretreatment function, and a freeze-dried reagent kit for cell staining, in order to perform DNA ploidy analysis easily and reproducibly in non-specialized laboratories. Biopsy specimens from brain tumors of 87 glioma patients who underwent open surgery were analyzed to evaluate the utility of our system. We were able to automatically analyze the biopsy specimens with the system within ten minutes. We compared the results of the same pretreated samples between our system and the conventional method using a common flow cytometer, confirming that the malignancy index was well correlated between them (R=0.975). These results demonstrated that our fully automated DNA ploidy analyzer is useful for rapid determination of glioma presence in surgical biopsy specimens.
Author 鈴木 あかね
野村 健一
Kobayashi Naoki
Kubo Hirotsugu
塩山 高広
新田 雅之
村垣 善浩
Komori Takashi
村上 知之
丸山 隆志
Author_FL Nitta Masayuki
Shioyama Takahiro
Murakami Tomoyuki
小林 直樹
Nomura Kennichi
Maruyama Takashi
小森 隆司
Muragaki Yoshihiro
Suzuki Akane
久保 寛嗣
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  fullname: Shioyama Takahiro
– sequence: 2
  fullname: Suzuki Akane
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  fullname: Nomura Kennichi
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  fullname: 久保 寛嗣
– sequence: 5
  fullname: 小林 直樹
– sequence: 6
  fullname: Nitta Masayuki
– sequence: 7
  fullname: Maruyama Takashi
– sequence: 8
  fullname: Murakami Tomoyuki
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  fullname: 小森 隆司
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  fullname: Muragaki Yoshihiro
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  fullname: 塩山 高広
  organization: Ogino Memorial Laboratory, Nihon Kohden Corporation, Tokyo, Japan | 日本光電工業株式会社　荻野記念研究所 | Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所
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  fullname: 鈴木 あかね
  organization: Ogino Memorial Laboratory, Nihon Kohden Corporation, Tokyo, Japan | 日本光電工業株式会社　荻野記念研究所 | Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所
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  fullname: 野村 健一
  organization: Ogino Memorial Laboratory, Nihon Kohden Corporation, Tokyo, Japan | 日本光電工業株式会社　荻野記念研究所 | Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所
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  fullname: Kubo Hirotsugu
  organization: 日本光電工業株式会社　荻野記念研究所 | Ogino Memorial Laboratory, Nihon Kohden Corporation, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所 | Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan
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  fullname: Kobayashi Naoki
  organization: Ogino Memorial Laboratory, Nihon Kohden Corporation, Tokyo, Japan | 日本光電工業株式会社　荻野記念研究所 | Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所
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  fullname: 新田 雅之
  organization: Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所 | Department of Neurosurgery, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　脳神経外科
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  fullname: 丸山 隆志
  organization: Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　先端生命医科学研究所 | Department of Neurosurgery, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　脳神経外科
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  fullname: 村上 知之
  organization: Department of Pathology, Kanmon Medical Center Shimonoseki, Japan | 独立行政法人　国立病院機構　関門医療センター　病理診断科
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  fullname: Komori Takashi
  organization: Department of Laboratory Medicine and Pathology (Neuropathology), Tokyo Metropolitan Neurological Hospital, Tokyo, Japan | 東京都立神経病院　検査科
– sequence: 10
  fullname: 村垣 善浩
  organization: 東京女子医科大学　先端生命医科学研究所 | Institute of Advanced Biomedical Engineering and Sience, Tokyo Women's Medical University, Tokyo, Japan | Department of Neurosurgery, Tokyo Women's Medical University, Tokyo, Japan | 東京女子医科大学　脳神経外科
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Snippet DNA ploidy analysis is one of the two principal applications of flow cytometry, along with cell surface antigen analysis. DNA ploidy analysis is positive in...
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StartPage 9
SubjectTerms DNA aneuploidy
DNA ploidy analysis automatic measurement system
Flow cytometry
glioma
Title 術中迅速フローサイトメトリーシステムの開発
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Volume 27
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