頭頸部扁平上皮癌細胞のBasic Fibroblast Growth Factor産生と血管新生
頭頸部扁平上皮癌培養細胞を用いて血管新生促進因子であるbasic fibroblast growth factor (bFGF) の産生能をみるとともに, 抗bFGF中和抗体の腫瘍細胞増殖抑制を検討した。またbFGF産生値と組織検体のF VIII RAG染色を用いた血管新生との相関性について検討した。 培養腫瘍細胞12株全ての培養上清中にbFGF産生が認められた。培養腫瘍細胞4種中2種で抗bFGF中和抗体による著明な増殖抑制を認めたが, recombinant human bFGFは腫瘍細胞の増殖には影響しなかった。培養腫瘍細胞のbFGF産生能と血管数とには相関性が認められ, bFGFは生体内...
Saved in:
| Published in | 耳鼻咽喉科展望 Vol. 36; no. 6; pp. 685 - 695 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Japanese |
| Published |
耳鼻咽喉科展望会
15.12.1993
|
| Online Access | Get full text |
| ISSN | 0386-9687 1883-6429 |
| DOI | 10.11453/orltokyo1958.36.685 |
Cover
| Abstract | 頭頸部扁平上皮癌培養細胞を用いて血管新生促進因子であるbasic fibroblast growth factor (bFGF) の産生能をみるとともに, 抗bFGF中和抗体の腫瘍細胞増殖抑制を検討した。またbFGF産生値と組織検体のF VIII RAG染色を用いた血管新生との相関性について検討した。 培養腫瘍細胞12株全ての培養上清中にbFGF産生が認められた。培養腫瘍細胞4種中2種で抗bFGF中和抗体による著明な増殖抑制を認めたが, recombinant human bFGFは腫瘍細胞の増殖には影響しなかった。培養腫瘍細胞のbFGF産生能と血管数とには相関性が認められ, bFGFは生体内の血管新生に関与する因子の一つであると考えられた。 以上のことからbFGFは血管新生因子の重要な一つであり, 腫瘍が産生するbFGFは, 腫瘍の増殖を増強する可能性があることが示唆された。 |
|---|---|
| AbstractList | 頭頸部扁平上皮癌培養細胞を用いて血管新生促進因子であるbasic fibroblast growth factor (bFGF) の産生能をみるとともに, 抗bFGF中和抗体の腫瘍細胞増殖抑制を検討した。またbFGF産生値と組織検体のF VIII RAG染色を用いた血管新生との相関性について検討した。 培養腫瘍細胞12株全ての培養上清中にbFGF産生が認められた。培養腫瘍細胞4種中2種で抗bFGF中和抗体による著明な増殖抑制を認めたが, recombinant human bFGFは腫瘍細胞の増殖には影響しなかった。培養腫瘍細胞のbFGF産生能と血管数とには相関性が認められ, bFGFは生体内の血管新生に関与する因子の一つであると考えられた。 以上のことからbFGFは血管新生因子の重要な一つであり, 腫瘍が産生するbFGFは, 腫瘍の増殖を増強する可能性があることが示唆された。 |
| Author | 三上, 康和 |
| Author_xml | – sequence: 1 fullname: 三上, 康和 organization: 横浜市立大学医学部耳鼻咽喉科学教室 |
| BookMark | eNpNkMFKAlEYhS9hkJlv0MIXGLsz13vnn2VJaiC0cT_89zqTY5MTdwbCXeKqcClBLUpp4yYRamHPYzn6FglGtDmHAx9n8e2TTCfqeIQcmrRomiXOjiIdJtFlNzIdDkUmigL4DsmaAMwQJcvJkCxlIAxHgL1H8nEcSEo52I4QdpY01qO39Wi-7k-Wd73vz_ev-X36NE0fB-nHbNV_XvSmJxgHqlAJpI5kiHFSqOroJmkVKqiSSKfD13T4suhNVuPbdDpePsw284Ds-hjGXv63c6RROW2Ua0b9vHpWPq4bbXDQULRpWwBSNRl4lClKQTHpowMCrZINDqO2L5XXBEv5nHGGaFlCCu5z5IIhy5Ha9rYdJ3jhudc6uELddVEngQo9978YlwlXbGPj5w9RLdRuG9kPxI94ag |
| ContentType | Journal Article |
| Copyright | 耳鼻咽喉科展望会 |
| Copyright_xml | – notice: 耳鼻咽喉科展望会 |
| DOI | 10.11453/orltokyo1958.36.685 |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| EISSN | 1883-6429 |
| EndPage | 695 |
| ExternalDocumentID | article_orltokyo1958_36_6_36_6_685_article_char_ja |
| GroupedDBID | ALMA_UNASSIGNED_HOLDINGS JSF RJT |
| ID | FETCH-LOGICAL-j89a-c0d7288bcd38e03c008c3bfa986a24789307fbced82cf5353aa226b65f5a563a3 |
| ISSN | 0386-9687 |
| IngestDate | Wed Sep 03 06:22:12 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 6 |
| Language | Japanese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-j89a-c0d7288bcd38e03c008c3bfa986a24789307fbced82cf5353aa226b65f5a563a3 |
| OpenAccessLink | https://www.jstage.jst.go.jp/article/orltokyo1958/36/6/36_6_685/_article/-char/ja |
| PageCount | 11 |
| ParticipantIDs | jstage_primary_article_orltokyo1958_36_6_36_6_685_article_char_ja |
| PublicationCentury | 1900 |
| PublicationDate | 1993/12/15 |
| PublicationDateYYYYMMDD | 1993-12-15 |
| PublicationDate_xml | – month: 12 year: 1993 text: 1993/12/15 day: 15 |
| PublicationDecade | 1990 |
| PublicationTitle | 耳鼻咽喉科展望 |
| PublicationTitleAlternate | 耳展 |
| PublicationYear | 1993 |
| Publisher | 耳鼻咽喉科展望会 |
| Publisher_xml | – name: 耳鼻咽喉科展望会 |
| References | 21) Okamura K, Sato Y, Matsuda T, et al: Endogenous basic fibroblast growth factordependent induction of collagenase and interleukin-6 in tumor necrosis factortreated human microvascular endothelial cells. J Biol Chem 266: 19162-49165, 1991. 7) 西川克三, 吉竹佳乃, 峯村正実, 他: 腫瘍由来ヘパリン結合性増殖因子の機能. 蛋白質核酸酵素36: 1364-1370, 1991. 16) Hayek A, Culler FL, Beattie GM, et al: An in vivo model for study of the angiogenic effects of basic fibroblast growth factor. Biochem Biophys Res Commun 147: 876-880, 1987. 17) Abraham JA, Mergia A, Whang JL, et al: Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor. Science 233: 545-548, 1986. 23) Chin JE, Hatfield CA, Krzesicki RF, et al: Interactions between interleukin-1 and basic fibroblast growth factor on articular chondrocytes. Arthritis Rheum 34: 314-324, 1991. 32) Takahashi JA, Fukumoto M, Igarashi K, et al: Correlation of basic fibroblast growth factor expression levels with the degree of malignancy and vascularity in human gliomas. J Neurosurg 76: 792-798, 1992. 24) BenEzra D, Hemo I, Maftzir G: In vivo angiogenic activity of interleukins. Arch Ophthalmol 108: 573-576, 1990. 6) Weidner N, Semple JP, Welch WR, et al: Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma. N Engl J Med 324: 1-8, 1991. 9) 笹野公伸, 宮崎修吉, 伊達文子, 他: AgNORs-病理組織診断への応用. 病理と臨床9: 890-893, 1991. 25) Gay CG, Winkles JA: Interleukin 1 regulates heparin-binding growth factor 2 gene expression in vascular smooth muscle cells. Proc Natl Acad Sci USA 88: 296-300, 1991. 12) Gospodarowicz D: Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3 T 3 cell growth. Nature 249: 123-127, 1974. 15) Montesano R, Vassalli JD, Baird A, et al: Basic fibroblast growth factor induces angiogenesis in vitro. Proc Natl Acad Sci USA 83: 7297-7301, 1986. 4) Gimbrone MA, Cotran RS, Leapman SB, et al: Tumor growth and neovascularization: An experimental model using the rabbit cornea. J Natl Cancer Inst 52: 413-427, 1974. 11) Howell WM, Black DA: Controlled silverstaining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015, 1980. 10) Hirsch SM, DuCanto J, Caldarelli DD, et al: Nucleolar organizer regions in squamous cell carcinoma of the head and neck. Laryngoscope 102: 39-44, 1992. 3) Knighton D, Ausprunk D, Tapper D, et al: Avascular and vascular phases of tumor growth in the chick embryo. Br J Cancer 35: 347-356, 1977. 26) Hori A, Sasada R, Matsutani E, et al: Suppression of solid tumor growth by immunoneutralizing monoclonal antibody against human basic fibroblast growth factor. Cancer Res 51: 6180-6184, 1991 13) Sato Y, Rifkin DB: Autocrine activities of basic fibroblast growth factor: regulation of endothelial cell movement, plasminogen activator synthesis, and DNA synthesis. J Cell Biol 107: 1199-1205, 1988. 20) Frater-Schroder M, Risau W, Hallmann R, et al: Tumor necrosis factor type α, a potent inhibitor of endothelial cell growth in vitro, is angiogenic in vivo. Proc Natl Acad Sci USA 84: 5277-5281, 1987. 2) Folkman J: Tumor angiogenesis. N Engl J Med 285: 1182-1186, 1971. 19) Friesel R, Komoriya A, Maciag T: Inhibition of endothelial cell proliferation by gamma-interferon. J Cell Biol 104: 689-696, 1987. 18) Watanabe H, Hori A, Seno M, et al: A sensitive enzyme immunoassay for human basic fibroblast growth factor. Biochem Biophys Res Commun 175: 229-235, 1991. 1) Folkman J: What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst 82: 4-6, 1990. 27) Takahashi JA, Fukumoto M, Kozai Y, et al: Inhibition of cell growth and tumorigenesis of human glioblastoma cells by a neutralizing antibody against human basic fibroblast growth factor. FEBS Lett 288: 65-71, 1991. 31) 三上康和, 佃守, 持松いつみ, 他: 頭頸部腫瘍における血管新生についての研究. 日耳鼻96: 645-650, 1993. 5) Srivastava A, Laidler P, Davies RP, et al: The prognostic significance of tumor vascularity in intermediate-thickness (0.76-4.0 mm thick) skin melanoma. Am J Pathol 133: 419-423, 1988. 14) Mignatti P, Tsuboi R, Robbins E, et al: In vitro angiogenesis on the human amniotic membrane: Requirement for basic fibroblast growth factor-induced proteinases. J Cell Biol 108: 671-682, 1989. 28) Gross JL, Herblin WF, Dusak BA, et al: Effects of modulation of basic fibroblast growth factor on tumor growth in vivo. J Natl Cancer Inst 85: 121-131, 1993. 22) Cozzolino F, Torcia M, Aldinucci D, et al: Interleukin 1 is an autocrine regulator of human endothelial cell growth. Proc Natl Acad Sci USA 87: 6487-6491, 1990. 8) Sato Y, Murphy PR, Sato R, et al: Fibroblast growth factor release by bovine endothelial cells and human astrocytoma cells in culture is density dependent. Mol Endocrinol 3: 744-748, 1989. 29) 吉竹佳乃, 西川克三: 腫瘍中のFGFとFGF遺伝子導入による発癌. 実験医学10: 351-355, 1992. 30) Weidner N, Folkman J, Pozza F, et al: Tumor angiogenesis: a new significant and independent prognostic indicator in earlystage breast carcinoma. J Natl Cancer Inst 84: 1875-1887, 1992. |
| References_xml | – reference: 23) Chin JE, Hatfield CA, Krzesicki RF, et al: Interactions between interleukin-1 and basic fibroblast growth factor on articular chondrocytes. Arthritis Rheum 34: 314-324, 1991. – reference: 5) Srivastava A, Laidler P, Davies RP, et al: The prognostic significance of tumor vascularity in intermediate-thickness (0.76-4.0 mm thick) skin melanoma. Am J Pathol 133: 419-423, 1988. – reference: 21) Okamura K, Sato Y, Matsuda T, et al: Endogenous basic fibroblast growth factordependent induction of collagenase and interleukin-6 in tumor necrosis factortreated human microvascular endothelial cells. J Biol Chem 266: 19162-49165, 1991. – reference: 10) Hirsch SM, DuCanto J, Caldarelli DD, et al: Nucleolar organizer regions in squamous cell carcinoma of the head and neck. Laryngoscope 102: 39-44, 1992. – reference: 6) Weidner N, Semple JP, Welch WR, et al: Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma. N Engl J Med 324: 1-8, 1991. – reference: 12) Gospodarowicz D: Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3 T 3 cell growth. Nature 249: 123-127, 1974. – reference: 9) 笹野公伸, 宮崎修吉, 伊達文子, 他: AgNORs-病理組織診断への応用. 病理と臨床9: 890-893, 1991. – reference: 4) Gimbrone MA, Cotran RS, Leapman SB, et al: Tumor growth and neovascularization: An experimental model using the rabbit cornea. J Natl Cancer Inst 52: 413-427, 1974. – reference: 16) Hayek A, Culler FL, Beattie GM, et al: An in vivo model for study of the angiogenic effects of basic fibroblast growth factor. Biochem Biophys Res Commun 147: 876-880, 1987. – reference: 15) Montesano R, Vassalli JD, Baird A, et al: Basic fibroblast growth factor induces angiogenesis in vitro. Proc Natl Acad Sci USA 83: 7297-7301, 1986. – reference: 29) 吉竹佳乃, 西川克三: 腫瘍中のFGFとFGF遺伝子導入による発癌. 実験医学10: 351-355, 1992. – reference: 25) Gay CG, Winkles JA: Interleukin 1 regulates heparin-binding growth factor 2 gene expression in vascular smooth muscle cells. Proc Natl Acad Sci USA 88: 296-300, 1991. – reference: 20) Frater-Schroder M, Risau W, Hallmann R, et al: Tumor necrosis factor type α, a potent inhibitor of endothelial cell growth in vitro, is angiogenic in vivo. Proc Natl Acad Sci USA 84: 5277-5281, 1987. – reference: 32) Takahashi JA, Fukumoto M, Igarashi K, et al: Correlation of basic fibroblast growth factor expression levels with the degree of malignancy and vascularity in human gliomas. J Neurosurg 76: 792-798, 1992. – reference: 2) Folkman J: Tumor angiogenesis. N Engl J Med 285: 1182-1186, 1971. – reference: 28) Gross JL, Herblin WF, Dusak BA, et al: Effects of modulation of basic fibroblast growth factor on tumor growth in vivo. J Natl Cancer Inst 85: 121-131, 1993. – reference: 19) Friesel R, Komoriya A, Maciag T: Inhibition of endothelial cell proliferation by gamma-interferon. J Cell Biol 104: 689-696, 1987. – reference: 3) Knighton D, Ausprunk D, Tapper D, et al: Avascular and vascular phases of tumor growth in the chick embryo. Br J Cancer 35: 347-356, 1977. – reference: 30) Weidner N, Folkman J, Pozza F, et al: Tumor angiogenesis: a new significant and independent prognostic indicator in earlystage breast carcinoma. J Natl Cancer Inst 84: 1875-1887, 1992. – reference: 13) Sato Y, Rifkin DB: Autocrine activities of basic fibroblast growth factor: regulation of endothelial cell movement, plasminogen activator synthesis, and DNA synthesis. J Cell Biol 107: 1199-1205, 1988. – reference: 27) Takahashi JA, Fukumoto M, Kozai Y, et al: Inhibition of cell growth and tumorigenesis of human glioblastoma cells by a neutralizing antibody against human basic fibroblast growth factor. FEBS Lett 288: 65-71, 1991. – reference: 17) Abraham JA, Mergia A, Whang JL, et al: Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor. Science 233: 545-548, 1986. – reference: 31) 三上康和, 佃守, 持松いつみ, 他: 頭頸部腫瘍における血管新生についての研究. 日耳鼻96: 645-650, 1993. – reference: 22) Cozzolino F, Torcia M, Aldinucci D, et al: Interleukin 1 is an autocrine regulator of human endothelial cell growth. Proc Natl Acad Sci USA 87: 6487-6491, 1990. – reference: 14) Mignatti P, Tsuboi R, Robbins E, et al: In vitro angiogenesis on the human amniotic membrane: Requirement for basic fibroblast growth factor-induced proteinases. J Cell Biol 108: 671-682, 1989. – reference: 8) Sato Y, Murphy PR, Sato R, et al: Fibroblast growth factor release by bovine endothelial cells and human astrocytoma cells in culture is density dependent. Mol Endocrinol 3: 744-748, 1989. – reference: 24) BenEzra D, Hemo I, Maftzir G: In vivo angiogenic activity of interleukins. Arch Ophthalmol 108: 573-576, 1990. – reference: 1) Folkman J: What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst 82: 4-6, 1990. – reference: 18) Watanabe H, Hori A, Seno M, et al: A sensitive enzyme immunoassay for human basic fibroblast growth factor. Biochem Biophys Res Commun 175: 229-235, 1991. – reference: 7) 西川克三, 吉竹佳乃, 峯村正実, 他: 腫瘍由来ヘパリン結合性増殖因子の機能. 蛋白質核酸酵素36: 1364-1370, 1991. – reference: 26) Hori A, Sasada R, Matsutani E, et al: Suppression of solid tumor growth by immunoneutralizing monoclonal antibody against human basic fibroblast growth factor. Cancer Res 51: 6180-6184, 1991 – reference: 11) Howell WM, Black DA: Controlled silverstaining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015, 1980. |
| SSID | ssib005879667 ssib005902162 ssib005879668 ssib058493089 ssib029852170 ssj0066717 ssib007484760 ssib002004161 |
| Score | 1.4140927 |
| Snippet | 頭頸部扁平上皮癌培養細胞を用いて血管新生促進因子であるbasic fibroblast growth factor (bFGF) の産生能をみるとともに, 抗bFGF中和抗体の腫瘍細胞増殖抑制を検... |
| SourceID | jstage |
| SourceType | Publisher |
| StartPage | 685 |
| Title | 頭頸部扁平上皮癌細胞のBasic Fibroblast Growth Factor産生と血管新生 |
| URI | https://www.jstage.jst.go.jp/article/orltokyo1958/36/6/36_6_685/_article/-char/ja |
| Volume | 36 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | 耳鼻咽喉科展望, 1993/12/15, Vol.36(6), pp.685-695 |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library customDbUrl: eissn: 1883-6429 dateEnd: 99991231 omitProxy: true ssIdentifier: ssib007484760 issn: 0386-9687 databaseCode: KQ8 dateStart: 19580101 isFulltext: true titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html providerName: Colorado Alliance of Research Libraries |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3Na9RAFA-1XryIouI3e3BOJTWb-cjMzck2S1EUhBV6C5NsgqzSLSUienLpSfFYBD1oi5deXAp6qH9Pten-F76ZyW6i9mDVS3hJZt7My5uP30vy3nOcGySFyx71XMKTwCUK1kGhlHABzGVero0xoh2F795jyw_I7RW6MnfiVtO7pEgW0-dH-pX8jVbhGuhVe8keQ7MzpnABaNAvHEHDcPwjHaNIIOkhuVQRIdcEx0gCwRAHuo0iikKBQowiogtwiaIACYlkZAgo09FECHc9FHFdXcAtrOvKKFSgxoUuGNXDBIB2oV9WPS0eLnRNnh7DgSCJK0J0pxW5ZiXb-kcKuAVtAQ1dEsy0UhVuImPTtGf6Cb3toDDUPRc-CpcMYcTRrKCuFaqNBDU8O0jMPhxNpRS1uDCITHmJwqDiyTv12w7zb2Hbd62_pxmf_9gX0zQQsrHKYs5cwapdP7O7AOfYBcNMNLcJG6elmg7NNZ_ZnEMVfGA2Z-jvOxMx-UWG64-L4aNnQx3jZxGzxVnln2J-VyMqbpaOMYuZPUCleFpEO-fFA7AQTvqwr-nkJXfuNwxIHU-t4blMeQD2bfDLeSOgmwDM1wggqSPNBrUB7QsOcK9e4QG9CuzpfAUW-wBrk-N69lArZ1Ut_M0jRAfYNwAjaPoDpcF0vTPO6coYa0kr5FlnbqDOOb3J1qfJ1t5kY-fg5ej718_f9l6V78bl29fll93Djff7o7GZE616TrTsnGjZOVFufiw3P-yPdg63X5Tj7YM3u3B63ul1o15n2a1yj7gDLpSbev3A5zxJ-5hnHk4BKac4yZXgTPkkAJDvBXmSZn3upznFFCsFdkzCaE4VZVjhC8786nA1u-i02jjPsaIKs1QRIlRCU9HXIbZyPyMZ9y850j6DeM3Gl4mPr_3L_4HHFedUPeGuOvPF-pPsGgDuIrluxtQP_F219A |
| linkProvider | Colorado Alliance of Research Libraries |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E9%A0%AD%E9%A0%B8%E9%83%A8%E6%89%81%E5%B9%B3%E4%B8%8A%E7%9A%AE%E7%99%8C%E7%B4%B0%E8%83%9E%E3%81%AEBasic+Fibroblast+Growth+Factor%E7%94%A3%E7%94%9F%E3%81%A8%E8%A1%80%E7%AE%A1%E6%96%B0%E7%94%9F&rft.jtitle=%E8%80%B3%E9%BC%BB%E5%92%BD%E5%96%89%E7%A7%91%E5%B1%95%E6%9C%9B&rft.au=%E4%B8%89%E4%B8%8A%2C+%E5%BA%B7%E5%92%8C&rft.date=1993-12-15&rft.pub=%E8%80%B3%E9%BC%BB%E5%92%BD%E5%96%89%E7%A7%91%E5%B1%95%E6%9C%9B%E4%BC%9A&rft.issn=0386-9687&rft.eissn=1883-6429&rft.volume=36&rft.issue=6&rft.spage=685&rft.epage=695&rft_id=info:doi/10.11453%2Forltokyo1958.36.685&rft.externalDocID=article_orltokyo1958_36_6_36_6_685_article_char_ja |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0386-9687&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0386-9687&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0386-9687&client=summon |