Diffusion Chamber型人工膵島における免疫隔離膜としての高分子膜の孔径の評価

Bioartificial endocrine pancreas (diffusion chamber型人工膵島)を構成する選択的透過膜の孔径の検討を行った. Diffusion chamberは, 厚さ0.5cm, 直径2.5cmのシリコシリングに選択的透過膜を上下より接着させた太鼓状のもので, その内腔に移植細胞(膵島細胞:MIN6)が封入されている. 選択的透過膜はアクリル製で, それぞれ直径0.2, 0.1および0.05μmの透過孔径を持った膜を用いた. さらに, chamber内に注入する細胞の機能維持を図るために培養基床(剤)としてagaroseや糖鎖を含有しpolymerおよびn...

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Published in人工臓器 Vol. 25; no. 4; pp. 794 - 797
Main Authors 佐藤, 純彦, 宮崎, 純一, 苅部, 幸代, 中川, 芳彦, 寺岡, 慧, 太田, 和夫, 赤池, 敏宏, 大河原, 久子, 広谷, 紗千子
Format Journal Article
LanguageJapanese
Published 一般社団法人 日本人工臓器学会 15.08.1996
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ISSN0300-0818
1883-6097
DOI10.11392/jsao1972.25.794

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Abstract Bioartificial endocrine pancreas (diffusion chamber型人工膵島)を構成する選択的透過膜の孔径の検討を行った. Diffusion chamberは, 厚さ0.5cm, 直径2.5cmのシリコシリングに選択的透過膜を上下より接着させた太鼓状のもので, その内腔に移植細胞(膵島細胞:MIN6)が封入されている. 選択的透過膜はアクリル製で, それぞれ直径0.2, 0.1および0.05μmの透過孔径を持った膜を用いた. さらに, chamber内に注入する細胞の機能維持を図るために培養基床(剤)としてagaroseや糖鎖を含有しpolymerおよびnicotinamideを用いた. 次に, それぞれの膜孔径を有するdiffusion chamber内MIN6-cellsを封入した後, 糖尿病ラット(streptozotocine-induced diabetic rats)の腹腔内に異種移植し, 血糖調節の可能性を検討した. その結果, diffusion chamber型人工膵島を構成する選択的透過膜の孔径(0.1μm)と移植細胞の機能維持を図る目的で使用した培養基剤との相互作用によって, 免疫抑制剤を使用することなく異種移植が可能であることが示唆された.
AbstractList Bioartificial endocrine pancreas (diffusion chamber型人工膵島)を構成する選択的透過膜の孔径の検討を行った. Diffusion chamberは, 厚さ0.5cm, 直径2.5cmのシリコシリングに選択的透過膜を上下より接着させた太鼓状のもので, その内腔に移植細胞(膵島細胞:MIN6)が封入されている. 選択的透過膜はアクリル製で, それぞれ直径0.2, 0.1および0.05μmの透過孔径を持った膜を用いた. さらに, chamber内に注入する細胞の機能維持を図るために培養基床(剤)としてagaroseや糖鎖を含有しpolymerおよびnicotinamideを用いた. 次に, それぞれの膜孔径を有するdiffusion chamber内MIN6-cellsを封入した後, 糖尿病ラット(streptozotocine-induced diabetic rats)の腹腔内に異種移植し, 血糖調節の可能性を検討した. その結果, diffusion chamber型人工膵島を構成する選択的透過膜の孔径(0.1μm)と移植細胞の機能維持を図る目的で使用した培養基剤との相互作用によって, 免疫抑制剤を使用することなく異種移植が可能であることが示唆された.
Author 大河原, 久子
広谷, 紗千子
中川, 芳彦
太田, 和夫
寺岡, 慧
赤池, 敏宏
佐藤, 純彦
苅部, 幸代
宮崎, 純一
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References 2) Ohgawara H, Kobayashi A, Kawamura M, Karibe S, Omori Y, Akaike T: Development of a method for embedded-culture of pig pancreatic islet-like cell clusters in agarose containing maltose-carrying polystyrene (HEVM) and nicotinamide. Cell Transplant 3: 83, 1994
6) Ohgawara H, Karibe S, Satoh A, Mochizuki N, Hirata Y: New type of bioartificial pancreas employing a diffusion chamber, Artificial Organs, eds by Ota K, Agishi T ISAO Press Cleveland 1990 14 (Suppl. 3): 11
13) Kobayashi A, Koyama A, Goto M, Kobayashi K, Chang CW, Tomita K, Akaike T: Glucose transporter-mediated dynamic attachment of erythrocytes onto a reducing glucose-carrying polystyrene. Proc Jpn Acad Ser B69: 89, 1993
18) LeDoux P, Hall R, Forbes M, Patton J, Wilson L: Mechanisms of nicotinamide and thymidine protection from alloxan and streptozotocin toxicity. Diabetes 37: 1015, 1988
19) Yamada K, Miyajima E, Nonaka K: Inhibition of cytokine-induced MHC class II but not class I molecula expression on mouse islet cells by nicotinamide and 3-aminobenzamide. Diabetes 39: 1125, 1990
16) Yomemura Y, Takashima T, Miwa K, Miyazaki I, Yamamoto H, Okamoto H: Amelioration of diabetes mellitus in partially depancreatized rat by poly (ADP-ribose) synthetase inhibitors. Diabetes 33: 401, 1984
7) Andersson A: Survival of pancreatic islet allografts. Lancet 2: 585, 1979
1) Ohgawara H, Miyazaki J, Karibe S, Tashiro F, Akaike T, Hashimoto Y: Embedded-culture of pancreatic B-cells derived from transgenic mouse insulinoma as a potential source for xenotransplantation using a diffusion chamber. Cell Transplant 4: 307, 1995
15) Okamoto H: Molecular basis of experimental diabetes: degeneration, oncogenesis and regeneration of pancreatic B-cells of islets of Langerhans. BioEssays 2: 15, 1985
5) Buschard K: Cultivation of islets of Langerhans in Millipore chamber in vivo. Horm Metab Res 7: 441, 1975
9) 小林明, 小林一清, 赤池敏宏: 細胞接着システムの新展開. 化学と生物 32: 260, 1994
8) 小林明, 大河原久子, 大森安恵, 後藤光昭, 小林一清, 赤池敏宏: 糖鎖を有した合成高分子含有ハイドロゲルのハイブリット型人工膵臓への応用. 人工臓器 22: 171, 1993
11) Kobayashi K, Kobayashi A, Akaike T: Culturing hepatocytes on lactose-carrying polystyrene layer asialo-glycoprotein receptor mediated interactions. Methods in Enzymology, ed by Lee YC, Lee RT Academic Press San Diego 1994, 409
3) Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, Oka Y, Yamamura K: Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion-special reference to expression of glucose transporter isoforms. Endocrinology 127: 126, 1990
10) Kobayashi K, Kobayashi A, Tobe S, Akaike T: Carbohydrate-containing polystyrene. Neoglycoconjugates: Preparation and Application, ed by Lee YC, Lee RT Academic Press San Diego 1994, 262
14) Yamamoto H, Uchigata Y, Okamoto H: Streptozotocin and alloxan induce DNA strand breaks and poly (ADP-ribose) synthesis in pancreatic islets. Nature 294: 284, 1981
17) Sugiyama K, Yomemura Y, Okamoto H: Effect of poly (ADP-ribose) synthetase inhibitor on B-cells of a canine pancreas after massive pancreatomy. Int J Pancreatol 8: 85, 1991
4) Bisceglie VV: Uber die antineoplastishe immunitat. E Krebsfursche 40: 141, 1933
12) Matsushita N, Oda H, Kobayashi K, Akaike T, Yoshida A: Induction of cytochrome P-450s and expression of liver-specific gene in rat primary hepatocytes cultured on different extracellular matricres. Biosci Biotechnol Biochem 58: 1514, 1994
References_xml – reference: 14) Yamamoto H, Uchigata Y, Okamoto H: Streptozotocin and alloxan induce DNA strand breaks and poly (ADP-ribose) synthesis in pancreatic islets. Nature 294: 284, 1981
– reference: 4) Bisceglie VV: Uber die antineoplastishe immunitat. E Krebsfursche 40: 141, 1933
– reference: 19) Yamada K, Miyajima E, Nonaka K: Inhibition of cytokine-induced MHC class II but not class I molecula expression on mouse islet cells by nicotinamide and 3-aminobenzamide. Diabetes 39: 1125, 1990
– reference: 11) Kobayashi K, Kobayashi A, Akaike T: Culturing hepatocytes on lactose-carrying polystyrene layer asialo-glycoprotein receptor mediated interactions. Methods in Enzymology, ed by Lee YC, Lee RT Academic Press San Diego 1994, 409
– reference: 5) Buschard K: Cultivation of islets of Langerhans in Millipore chamber in vivo. Horm Metab Res 7: 441, 1975
– reference: 18) LeDoux P, Hall R, Forbes M, Patton J, Wilson L: Mechanisms of nicotinamide and thymidine protection from alloxan and streptozotocin toxicity. Diabetes 37: 1015, 1988
– reference: 13) Kobayashi A, Koyama A, Goto M, Kobayashi K, Chang CW, Tomita K, Akaike T: Glucose transporter-mediated dynamic attachment of erythrocytes onto a reducing glucose-carrying polystyrene. Proc Jpn Acad Ser B69: 89, 1993
– reference: 6) Ohgawara H, Karibe S, Satoh A, Mochizuki N, Hirata Y: New type of bioartificial pancreas employing a diffusion chamber, Artificial Organs, eds by Ota K, Agishi T ISAO Press Cleveland 1990 14 (Suppl. 3): 11
– reference: 8) 小林明, 大河原久子, 大森安恵, 後藤光昭, 小林一清, 赤池敏宏: 糖鎖を有した合成高分子含有ハイドロゲルのハイブリット型人工膵臓への応用. 人工臓器 22: 171, 1993
– reference: 3) Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, Oka Y, Yamamura K: Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion-special reference to expression of glucose transporter isoforms. Endocrinology 127: 126, 1990
– reference: 10) Kobayashi K, Kobayashi A, Tobe S, Akaike T: Carbohydrate-containing polystyrene. Neoglycoconjugates: Preparation and Application, ed by Lee YC, Lee RT Academic Press San Diego 1994, 262
– reference: 7) Andersson A: Survival of pancreatic islet allografts. Lancet 2: 585, 1979
– reference: 12) Matsushita N, Oda H, Kobayashi K, Akaike T, Yoshida A: Induction of cytochrome P-450s and expression of liver-specific gene in rat primary hepatocytes cultured on different extracellular matricres. Biosci Biotechnol Biochem 58: 1514, 1994
– reference: 9) 小林明, 小林一清, 赤池敏宏: 細胞接着システムの新展開. 化学と生物 32: 260, 1994
– reference: 16) Yomemura Y, Takashima T, Miwa K, Miyazaki I, Yamamoto H, Okamoto H: Amelioration of diabetes mellitus in partially depancreatized rat by poly (ADP-ribose) synthetase inhibitors. Diabetes 33: 401, 1984
– reference: 17) Sugiyama K, Yomemura Y, Okamoto H: Effect of poly (ADP-ribose) synthetase inhibitor on B-cells of a canine pancreas after massive pancreatomy. Int J Pancreatol 8: 85, 1991
– reference: 1) Ohgawara H, Miyazaki J, Karibe S, Tashiro F, Akaike T, Hashimoto Y: Embedded-culture of pancreatic B-cells derived from transgenic mouse insulinoma as a potential source for xenotransplantation using a diffusion chamber. Cell Transplant 4: 307, 1995
– reference: 2) Ohgawara H, Kobayashi A, Kawamura M, Karibe S, Omori Y, Akaike T: Development of a method for embedded-culture of pig pancreatic islet-like cell clusters in agarose containing maltose-carrying polystyrene (HEVM) and nicotinamide. Cell Transplant 3: 83, 1994
– reference: 15) Okamoto H: Molecular basis of experimental diabetes: degeneration, oncogenesis and regeneration of pancreatic B-cells of islets of Langerhans. BioEssays 2: 15, 1985
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Snippet Bioartificial endocrine pancreas (diffusion chamber型人工膵島)を構成する選択的透過膜の孔径の検討を行った. Diffusion chamberは, 厚さ0.5cm, 直...
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Title Diffusion Chamber型人工膵島における免疫隔離膜としての高分子膜の孔径の評価
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