INHIBITORY EFFECT OF A STREPTOCOCCAL PREPARATION (OK-432) ON THE NUCLEIC ACID SYNTHESIS IN TUMOR CELLS IN VITRO

• Published in: GANN Japanese Journal of Cancer Research Vol. 64; no. 1; pp. 59 - 69
• Main Authors: KURATA, Sumiko, ONO, Tetsuo, SAITO, Motoo, OGAWA, Haruki, SUGAWARA, Yutaka, WAKABAYASHI, Kiyoshige
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Cancer Association 01.02.1973; 日本癌学会
• Subjects: Animals; Carcinoma - metabolism; Carcinoma, Ehrlich Tumor - metabolism; Carcinoma, Hepatocellular - metabolism; DNA, Neoplasm - biosynthesis; HeLa Cells - metabolism; Hot Temperature; Humans; In Vitro Techniques; Liver Neoplasms - metabolism; Lymphocytes - metabolism; Mice; Nasopharyngeal Neoplasms - metabolism; Neoplasms, Experimental - metabolism; Rats; RNA, Neoplasm - biosynthesis; Sarcoma, Yoshida - metabolism; Streptococcus - immunology; Streptococcus pyogenes - immunology; Thymidine - metabolism; Tritium; Uridine - metabolism
• ISSN: 0016-450X
• DOI: 10.20772/cancersci1959.64.1_59

The in vitro effect of OK-432 (NSC-B116209), the lyophilized preparation of penicillin-treated cell suspension of a low virulent strain, Su, of Streptococcus haemolyticus, on the metabolism of rat Yoshida sarcoma, ascites hepatoma AH-130, and mouse Ehrlich carcinoma cells was investigated. It was confirmed that living cells of Su strain released RNA from the tumor cells by contact in vitro, but OK-432 did not exert such an action at all. However, OK-432 was found to inhibit RNA synthesis and also DNA synthesis to a lesser extent in tumor cells in vitro in proportion to the dose of OK-432 applied. The effect of OK-432 on the RNA synthesis was compared using human malignant cells, HeLa, and nasopharyngeal carcinoma (NPC-204) cells, and phytohemagglutinin-stimulated normal human lymphocytes in culture. Among them, nasopharyngeal carcinoma cells were the most susceptible to the inhibition by OK-432. Protein synthesis of the carcinoma cells was also found to be highly sensitive to OK-432. These data seem to indicate that the extent of inhibition of RNA synthesis by OK-432 varies according to the cells tested and is roughly correlated to the susceptibility of tumor cells in vivo.