オートファジは胎仔マウス顎下腺の分枝形態形成に関与している

• Published in: 日本薬理学会年会要旨集 p. 1-B-P-070
• Main Authors: 柏俣, 正典, 足立, 圭亮
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2023
• Subjects: その他
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.97.0_1-B-P-070

Autophagy is defined as a mechanism that transports cellular components to lysosomes for degradation. This mechanism also plays a role in the removal of unwanted organelles, in addition to providing nutrients as a starvation response by recycling the degradation products. The mouse submandibular gland (SMG) rudiment is very small organ such as approximately 300 µm in diameter at embryonic day 13 (E13). The epithelium of SMG rudiment then undergoes active proliferation in contact with the mesenchyme and develop the duct systems in the gland (branching morphogenesis). In this study, we investigated whether autophagy is involved during salivary gland organogenesis. The organs of E13 SMG rudiments were cultured with Bafilomycin A1 or Torin 1 which are inhibitor or promoter of autophagy reactions, respectively. Cultured SMGs were photographed, counted the number of endpieces and analyzed the area of epithelial rudiments. Moreover, the autophagy related proteins such as Atg5, LC3-Ⅰ/Ⅱ and p62 were analyzed by Western blotting. Bafilomycin A1 suppressed branching morphogenesis of E13 SMG rudiments. The suppression of branching morphogenesis was also showed in administration of Torin 1 in the rudiments. The Atg5, LC3-Ⅰ/Ⅱ and p62 proteins in cultured SMG rudiments were induced by Bafilomycin A1. These results suggest that autophagy is involved in the branching morphogenesis of developing fetal mouse SMG.