リーダーRNA相補的ホスホロチオエートオリゴヌクレオチドによるマウス肝炎ウイルスの増殖抑制

マウス肝炎ウイルス(MHV)のリーダーRNAに相補的なホスホロチオエートオリゴヌクレオチド (PS-oligo) は低濃度(0.001～0.1μM)で配列特異的に通常のオリゴヌクレオチドより効率的にMHVの増殖を抑制した. ランダム配列やホモ配列(dC)のPS-oligoは高濃度(0.5μM以上)で有意なMHVの増殖抑制効果を示し, PS-oligoは配列特異的ならびに配列非特異的な2種類の異なった機序でMHVの増殖を阻害することが示された....

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Published inJournal of Veterinary Medical Science Vol. 57; no. 6; pp. 1081 - 1083
Main Authors 林, 正信, 荒井, 惣一郎, 野崎, 周英, 花木, 賢一, 前田, 秋彦, 渡邊, 智正
Format Journal Article
LanguageEnglish
Published 公益社団法人 日本獣医学会 1995
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ISSN0916-7250
1347-7439
DOI10.1292/jvms.57.1081

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Abstract マウス肝炎ウイルス(MHV)のリーダーRNAに相補的なホスホロチオエートオリゴヌクレオチド (PS-oligo) は低濃度(0.001～0.1μM)で配列特異的に通常のオリゴヌクレオチドより効率的にMHVの増殖を抑制した. ランダム配列やホモ配列(dC)のPS-oligoは高濃度(0.5μM以上)で有意なMHVの増殖抑制効果を示し, PS-oligoは配列特異的ならびに配列非特異的な2種類の異なった機序でMHVの増殖を阻害することが示された.
AbstractList マウス肝炎ウイルス(MHV)のリーダーRNAに相補的なホスホロチオエートオリゴヌクレオチド (PS-oligo) は低濃度(0.001～0.1μM)で配列特異的に通常のオリゴヌクレオチドより効率的にMHVの増殖を抑制した. ランダム配列やホモ配列(dC)のPS-oligoは高濃度(0.5μM以上)で有意なMHVの増殖抑制効果を示し, PS-oligoは配列特異的ならびに配列非特異的な2種類の異なった機序でMHVの増殖を阻害することが示された.
Author 渡邊, 智正
花木, 賢一
野崎, 周英
前田, 秋彦
荒井, 惣一郎
林, 正信
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– reference: Cohen, J. S. 1991. Antiviral Res. 16: 121-133.
– reference: Wickstrom, E. 1986. J. Biochem. Biophys. Meth. 13: 97-102.
– reference: Lai, M. M. C., Patton, C. D., Baric, R. S., and Stohlman, S. A. 1983. J. Virol. 48: 1027-1033.
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– reference: Agrawal, S. and Sarin, P. S. 1991. Adv. Drug Delivery Rev. 6: 251-270.
– reference: Pachuk, C. K., Bredenbeak, P. J., Zoltick, P. W., Spaan, W. J. M., and Weiss, S. R. 1989. Virology 171: 141-148.
– reference: Zerial, A., Thuong, N. T., and Helene, C. 1987. Nucleic Acid Res. 15: 9909-9919.
– reference: Makino, S., Taguchi, F., Hayami, M., and Fujiwara, K. 1983. Microbiol. lmmunol. 27: 445-454.
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Snippet マウス肝炎ウイルス(MHV)のリーダーRNAに相補的なホスホロチオエートオリゴヌクレオチド (PS-oligo) は低濃度(0.001～0.1μM)で配列特異的に通常のオリゴヌクレオチドより...
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Title リーダーRNA相補的ホスホロチオエートオリゴヌクレオチドによるマウス肝炎ウイルスの増殖抑制
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