発酵月桃熱水抽出物のB16マウスメラノーマ細胞におけるメラニン生成に対する影響

  月桃（Alpinia speciosa K. Schum.）は，古くから沖縄では虫よけ剤，抗菌剤などに使われてきた植物であり，その葉のもつ独特な香りを改善し，有効性を高めた発酵月桃が開発されている．本研究は発酵月桃葉熱水抽出物（HFS）のメラニン生成抑制作用とそのメカニズムを検討することを目的とした． HFSはB16細胞の細胞増殖には影響を与えず，濃度依存的にメラニン生成を抑制し，その抑制効果はアスコルビン酸（AsA）よりも大きいことが示唆された．次に，メラニン生成の律速酵素であるチロシナーゼに対す影響を調べた結果，チロシナーゼの反応には直接作用しないが，細胞内のチロシナーゼ活性を低下させ...

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Published in日本家政学会誌 Vol. 64; no. 5; pp. 215 - 224
Main Authors 藤原, 葉子, 与那, 覇恵, 岩渕, まり, 青木, 由美, 長幡, 友実
Format Journal Article
LanguageJapanese
Published 一般社団法人 日本家政学会 2013
Subjects
B16 メラノーマ細胞
チロシナーゼ
メラニン
月桃
Online AccessGet full text
ISSN0913-5227
1882-0352
DOI10.11428/jhej.64.215

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Abstract   月桃（Alpinia speciosa K. Schum.）は，古くから沖縄では虫よけ剤，抗菌剤などに使われてきた植物であり，その葉のもつ独特な香りを改善し，有効性を高めた発酵月桃が開発されている．本研究は発酵月桃葉熱水抽出物（HFS）のメラニン生成抑制作用とそのメカニズムを検討することを目的とした． HFSはB16細胞の細胞増殖には影響を与えず，濃度依存的にメラニン生成を抑制し，その抑制効果はアスコルビン酸（AsA）よりも大きいことが示唆された．次に，メラニン生成の律速酵素であるチロシナーゼに対す影響を調べた結果，チロシナーゼの反応には直接作用しないが，細胞内のチロシナーゼ活性を低下させた．さらにB16細胞のチロシナーゼmRNAとタンパク質発現量を低下させた． また，一連のメラニン産生タンパク質発現を制御する転写因子MITFの発現も減少したことから，発酵月桃葉熱水抽出物はMITFを介した転写レベルでチロシナーゼの発現を抑制し，メラニン生成を抑制することが示唆された．
AbstractList   月桃（Alpinia speciosa K. Schum.）は，古くから沖縄では虫よけ剤，抗菌剤などに使われてきた植物であり，その葉のもつ独特な香りを改善し，有効性を高めた発酵月桃が開発されている．本研究は発酵月桃葉熱水抽出物（HFS）のメラニン生成抑制作用とそのメカニズムを検討することを目的とした． HFSはB16細胞の細胞増殖には影響を与えず，濃度依存的にメラニン生成を抑制し，その抑制効果はアスコルビン酸（AsA）よりも大きいことが示唆された．次に，メラニン生成の律速酵素であるチロシナーゼに対す影響を調べた結果，チロシナーゼの反応には直接作用しないが，細胞内のチロシナーゼ活性を低下させた．さらにB16細胞のチロシナーゼmRNAとタンパク質発現量を低下させた． また，一連のメラニン産生タンパク質発現を制御する転写因子MITFの発現も減少したことから，発酵月桃葉熱水抽出物はMITFを介した転写レベルでチロシナーゼの発現を抑制し，メラニン生成を抑制することが示唆された．
Author 青木, 由美
藤原, 葉子
岩渕, まり
与那, 覇恵
長幡, 友実
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1) Urabe, K.; Nakayama, J.; Hori, Y.; “The Pigmentary System: Physiology and Pathophysiology.” eds. Nordlund, JJ.; Boissy, RE.; Hearing, VJ.;King, RA.; Ortonne, JP.; Oxford University Press, New York, 1998, p.909-911
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References_xml – reference: 24) Tachibana, M.; MITF: A stream flowing for pigment cells. Pigment Cell Res., 2000, vol.13, p.230-240
– reference: 36) Englaro, W.; Rezzonico, R.; Durand-Clement, M.; Lallemand, D.; Ortonner, J.P.; Ballotti, R.; Mitogenactivated protein kinase pathway and AP-1 are activated during cAMP-induced melanogenesis in B-16 melanoma cells. J. Biol. Chem., 1995, vol.270, no.41, p.24315-24320
– reference: 39) Chompoo, J.; Upadhyay, A.; Fukuta, M.; Tawata, S.; Effect of Alpinia zerumbet components on antioxidant and skin diseases-related enzymes. BMC Complementary ＆ Alternative Medicine., 2012, vol.12, p.106-114
– reference: 40) Murakami, A.; Ishida, H.; Kobo, K.; Furukawa, I.; Ikeda, Y.; Yonaha, M.; Aniya, Y.; Ohigashi, H.; Suppressive effects of Okinawa food items on free radical generation from stimulated leukocytes and identification of some active constituents: implication for the prevention of inflammation-associated carcinogenesis. Asian. Pc. J. Cancer. Prev., 2005, vol. 6, p.437-448
– reference: 7) Cabanes, J.; Chazarra, S.; Garcia-Carmona, F.; Kojic acid, a cosmetic skin whitening agent, is a slowbinding inhibitor of catecholase activity of tyrosinase. J. Pharm. Pharmacol., 1994, vol.46, no.12, p.982-985
– reference: 8) Ando, H.; Watabe, H.; Valencia, CJ.; Yasumoto, K.; Furumura, M.; Funasaka, Y.; Oka, M.; Ichihashi, M.; Hearing, JV.; Fatty acids regulate pigmentation via proteasomal degradation of tyrosinase. J. Biol. Chem. 2004, vol.279, no.15, p.15427-15433
– reference: 10) Kim, KS.; Kim, JA.; Eom, SY.; Lee, AH.; Min, KR.; Kim, Y.; Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by down regulation of tyrosinase expression., Pigment Cell Res., 2006, vol.19, p.90-98
– reference: 38) Liao, MC.; Arakaki, H.; Li, Y.; Takamiyagi, A.; Tawata, S.; Aniya, Y.; Sakurai, H.; Nonaka, S.; Inhibitory effects of Alpinia speciosa K. SCHUM on the porphyrin photooxidative reaction. J. Dematol., 2000, vol.27, p.312-317
– reference: 6) Shimogaki, H.; Tanaka, Y.; Tamai, H.; Masuda, M.; In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition., Int. J. Cosmet. Sci., 2000, vol.22, p.291-303
– reference: 4) Hearing, VJ.; Jimenez, M.; Analysis of mammalian pigmentation at the molecular level. Pigment. Cell. Res., 1989, vol.2, p.75-85
– reference: 15) Masuda, T.; Mizuguchi, S.; Tanaka, T.; Iritani, K.; Takeda, Y.; Isolation and structure determination of new antioxidative ferulic acid glucoside esters from the rhizome of Alpinia speciosa, a zingiberaceae plant used in Okinawan food culture. J. Agric. Food Chem., 2000, vol.48, no.5, p.1479-1484.
– reference: 18) Aida, K.; Shinmoto, H.; Kobori, M.; Tsushida, T.; Purification and identification of melanogenesis inhibitory factors in miso. Nippon Shokuhin Kagaku Kogaku Kaishi (in Japanese), 1998, vol.45, no.3, p.205-209
– reference: 30) Aksan, I.; Goding, CR.; Targeting the microphthalmia basic helix-loop-helix-leucine zipper transcription factor to a subset of e-box elements. Mol. Cell. Biol., 1998, vol.18, no.12, p.6930-6938
– reference: 14) Ishida, Y.; Kishida, N.; Japan Patent, 4658290 (Jan.7, 2011).
– reference: 1) Urabe, K.; Nakayama, J.; Hori, Y.; “The Pigmentary System: Physiology and Pathophysiology.” eds. Nordlund, JJ.; Boissy, RE.; Hearing, VJ.;King, RA.; Ortonne, JP.; Oxford University Press, New York, 1998, p.909-911
– reference: 37) Tawata, S.; Taira, S.; Kobamoto, N.; Ishihara, M.; Toyama, S.; Syntheses and biological activities of dihydro-5,6-dehydrokawain derivatives. Biosci. Biotechnol. Biochem., 1996, vol.60, no.10, p.1643-1645
– reference: 17) Chen, YC.; Sugiyama, Y.; Abe, N.; Kuruto-Niwa, R.; Nozawa, R.; Hirota, A.; DPPH radical-scavenging compounds from dou-chi, a soybean fermented food. Biosci. Biotechnol. Biochem., 2005, vol.69, no.5, p.999-1006.
– reference: 23) Yasumoto, K.; Yokoyama, K.; Takahashi, K.; Tomita, Y.; Shibahara, S.; Functional analysis of microphthalmiaassociated transcription factor in pigment cellspecific transcription of the human tyrosinase family genes. J. Biol. Chem., 1997, vol.272, no.1, p.503-509
– reference: 26) Pawelek, JM.; Factors regulating growth and pigmentation of melanoma cells. J. Invest. Dermatol., 1976, vol.66, no.4, p.201-209
– reference: 28) Hemesath, TJ.; Steingrimsson, E.; McGill, G.; Hansen, MJ.; Vaught, J.; Hodgkinson, CA.; Arnheiter, H.; Copeland NG.; Jenkins, NA.; Fisher, DE.; Microphthalmia, a critical factor in melanocyte development, defines a discrete transcription factor family. Gene. Dev., 1994, vol.8, p.2770-2780
– reference: 34) Halaban, R.; Rubin, JS.; Funasaka, Y.; Cobb, M.; Boulton, T.; Faletto, D.; Rosen, E.; Chan, A.; Yoko, K.; White, W.; Cook, C.; Moellmann, G.; Met and hepatocyte growth factor/scatter factor signal transduction in normal melanocytes and melanoma cells. Oncogene, 1992, vol.7, no.11, p.2195-2206
– reference: 3) Hearing. VJ.; Biochemical control of melanogenesis and melanosomal organization. J. Investig. Dermatol. Symp. Proc., 1999, vol.4, no.1, p.24-28
– reference: 9) Ando, H.; Wen, ZM.; Kim, HY.; Valencia, CJ.; Costin, GE.; Watabe, H.; Yasumoto, K.; Niki, Y.; Kondoh. H.; Ichihashi, M.; Hearing, VJ.; Intracellular composition of fatty acid affects the processing and function of tyrosinase through the ubiquitin-proteasome pathway. Biochem. J. 2006, vol.394, p.43-50
– reference: 29) Nobukuni, Y.; Watanabe, A.; Takeda, K.; Skarka, H.; Tachibana, M.; Analyses of loss-of-function mutations of the MITF gene suggest that haploinsufficiency is a cause of Waardenburg syndrome type 2A. Am. J. Hum. Genet., 1996, vol.59, no.1, p.76-83
– reference: 13) Laranja, SMR.; Bergamaschi, CM.; Schor, N.; Evaluation of acute administration of natural products with potential diuretic effects, in humans. Mem.Inst. Oswaldo Cruz., 1991, vol.86, p.237-240.
– reference: 25) Park, HY.; Gilchrest, B.; Signaling pathways mediating melanogenesis. Cell. Mol. Biol., 1999, vol.45, p.230-240
– reference: 16) Esaki, H.; Kawakishi, S.; Morimitsu, Y.; Osawa, T.; New potent antioxidative o-dihydroxyisoflavones in fermented Japanese soybean products. Biosci. Biotechnol. Biochem., 1999, vol.63, no.9 p.1637-1639.
– reference: 20) Brunk, CF.; Jones, KC.; James, TW.; Assay for nanogram quantities of DNA in cellular homogenates. Anal. Biochem., 1979, vol.92, p.497-500
– reference: 5) Maeda,K.; Fukuda, M.; Arbutin: mechanism of its depigmenting action in human melanocyte culture. J.Pharmacol. Exp. Ther., 1996, vol.276, no.2, p.765-769
– reference: 19) Inafuku, S.; Fujino, T.; Arime, O.; Yonaha, M.; Japan Patent, 4031637 (Oct.26, 2007).
– reference: 22) Yasumoto, K.; Yokoyama, K.; Shibata, K.; Tomita, Y.; Shibahara, S.; Microphthalmia-associated transcription factor as a regulator for melanocytespecific transcription of the human tyrosinase gene. Mol. Cell. Biol., 1994, vol.14, no.12, p.8058-8070
– reference: 27) Fujiwara, Y.; Inafuku, S.; Inafuku, N.; Fujino, T.; Yonaha, M.; Higa, M.; Seyama, Y.; Aoki, Y.; Japan Patent, 4942322 (Mar.9, 2012)
– reference: 31) Hemesath, TJ.; Price, ER.; Takemoto, C.; Badalian, T.; Fisher, DE.; MAP kinase links the transcription factor microphthalmia to c-kit signaling in melanocytes. Nature, 1998, vol.391, p.298-301
– reference: 21) Lowry, OH.; Rosebrough, NJ.; Farr, AL.; Randall, RJ.; Protein measurement with the folin phenol reagent. J.Biol. Chem., 1951, vol.193, p.265-275
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Snippet   月桃（Alpinia speciosa K. Schum.）は，古くから沖縄では虫よけ剤，抗菌剤などに使われてきた植物であり，その葉のもつ独特な香りを改善し，有効性を高めた発酵月桃が...
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StartPage 215
SubjectTerms B16 メラノーマ細胞
チロシナーゼ
メラニン
月桃
Title 発酵月桃熱水抽出物のB16マウスメラノーマ細胞におけるメラニン生成に対する影響
URI https://www.jstage.jst.go.jp/article/jhej/64/5/64_215/_article/-char/ja
Volume 64
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ispartofPNX 日本家政学会誌, 2013, Vol.64(5), pp.215-224
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