QUANTITATIVE ANALYSIS OF URINARY ORGANIC ACIDS BY STABLE ISOTOPE DILUTION METHOD: APPLICATION AS ROUTINE SCREENING TEST FOR METABOLIC DISORDERS
We describe a quantitative analysis of major urinary organic acids by stable isotope dilution method as a chemical diagnosis of metabolic disorders. In this report, we discuss extraction procedure recoveries, accuracy and analytical recovery by this method, and the effects of urinary albumin and uri...
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| Published in | Japanese Journal of National Medical Services Vol. 46; no. 5; pp. 317 - 323 |
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| Main Authors | , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japanese Society of National Medical Services
1992
一般社団法人 国立医療学会 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0021-1699 1884-8729 |
| DOI | 10.11261/iryo1946.46.317 |
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| Abstract | We describe a quantitative analysis of major urinary organic acids by stable isotope dilution method as a chemical diagnosis of metabolic disorders. In this report, we discuss extraction procedure recoveries, accuracy and analytical recovery by this method, and the effects of urinary albumin and urinary pH values. The solvent extraction can be simply and rapidly prepared during routine screening test, although pyrimidine metabolites and citric acid and pyroglutamic acid levels yielded were extremely low. The accuracy and analytical recovery of this method were found to be good. There was no effect of urinary albumin below the levels of 1g/l, and optimal pH was 0.5 to 1.0. Change in excreted levels of some acids were observed when the subjects were on unrestricted diets. In comparison with the conventional method, this method has the advantage of being an analytical procedure which can supply a useful data in the differential diagnosis of metabolic disorders with similar profiles of urinary organic acids. |
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| AbstractList | We describe a quantitative analysis of major urinary organic acids by stable isotope dilution method as a chemical diagnosis of metabolic disorders. In this report, we discuss extraction procedure recoveries, accuracy and analytical recovery by this method, and the effects of urinary albumin and urinary pH values. The solvent extraction can be simply and rapidly prepared during routine screening test, although pyrimidine metabolites and citric acid and pyroglutamic acid levels yielded were extremely low. The accuracy and analytical recovery of this method were found to be good. There was no effect of urinary albumin below the levels of 1g/l, and optimal pH was 0.5 to 1.0. Change in excreted levels of some acids were observed when the subjects were on unrestricted diets. In comparison with the conventional method, this method has the advantage of being an analytical procedure which can supply a useful data in the differential diagnosis of metabolic disorders with similar profiles of urinary organic acids.
今回, 我々はガスクロマトグラフィー/質量分析(GC/MS)による有機酸代謝異常症の院内ルーチン検査への導入に際し, 問題となる点を明確にするとともに, その改良法として安定同位体希釈法による高精度な尿中有機酸定量分析を試みた. 検討項目としては, 有機溶媒による各種有機酸の抽出効率, 本法の再現性, 添加回収率, 尿中共存物質およびpHによる抽出効率の変化を取り上げた.有機溶媒抽出は日常検査の前処理法としては簡便であるが, クロマト法に比ベクエン酸やオロット酸などのように, 抽出率が極端に低下するものもあったが, 安定同位体希釈法による再現性, 添加回収率については満足すべき結果が得られた. 尿中共存物質の影響として, アルブミンを取り上げたが, 1g/Lの濃度まで定量値に影響はなく, 抽出時の尿pHは0.5付近が最適であると思われた. 食事による定量値への影響については, クエン酸, フマル酸の有意な上昇が認められた以外は, ほとんど変動はなかった.本法による尿中有機酸定量分析は, 従来法に比べて種々の利点を有しており, 有機酸プロフィルの類似した代謝性疾患の鑑別診断法として臨床応用可能であると思われた. We describe a quantitative analysis of major urinary organic acids by stable isotope dilution method as a chemical diagnosis of metabolic disorders. In this report, we discuss extraction procedure recoveries, accuracy and analytical recovery by this method, and the effects of urinary albumin and urinary pH values. The solvent extraction can be simply and rapidly prepared during routine screening test, although pyrimidine metabolites and citric acid and pyroglutamic acid levels yielded were extremely low. The accuracy and analytical recovery of this method were found to be good. There was no effect of urinary albumin below the levels of 1g/l, and optimal pH was 0.5 to 1.0. Change in excreted levels of some acids were observed when the subjects were on unrestricted diets. In comparison with the conventional method, this method has the advantage of being an analytical procedure which can supply a useful data in the differential diagnosis of metabolic disorders with similar profiles of urinary organic acids. |
| Author | SHIMIZU, Akira NAKANISHI, Toyofumi |
| Author_FL | 清水 章 中西 豊文 |
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| References | 2) J Greter et al: Urinary organic acids: Isolation and quantification for routine metabolic screening, Clin Chem, 33: 473, 1987 4) G Hoffmann et al: Quantitative analysis for organic acids in biological samples Batch isolation followed by gas chromatographic-mass spectrometric analysis, Clin Chem, 35: 587, 1989 5) K Tanaka et al: Gas chromatographic method of analysis for urinary organic acids I. II., Clin Chem, 26: 1839, 1980 1) T Nakanishi et al: Urinary organic acids in elderly Japanese patients with ketosis and encephalopathy who have taken pantoyl-gamma-aminobutyrate, calcium salt (calcium hopantenate, HOPA), Clin Chim Acta, 188: 85, 1990 7) RA Chalmers et al: Urinary oranic acids in man: Effects of individual variation and diet on urinary excretion of acidic metabolites, Clin Chem, 22: 1288, 1976 6) T Nakanishi et al: Quantitative analysis of urinary pyroglutamic acid in hyperammonemia, Clin Chim Acta, 197: 249, 1991 3) BJ Verhaehge et al: Solid-phase extraction with strong anion-exchange columns for selective isolation and concentration of urinary organic acids, Clin Chem, 34: 1077, 1988 |
| References_xml | – reference: 7) RA Chalmers et al: Urinary oranic acids in man: Effects of individual variation and diet on urinary excretion of acidic metabolites, Clin Chem, 22: 1288, 1976 – reference: 5) K Tanaka et al: Gas chromatographic method of analysis for urinary organic acids I. II., Clin Chem, 26: 1839, 1980 – reference: 3) BJ Verhaehge et al: Solid-phase extraction with strong anion-exchange columns for selective isolation and concentration of urinary organic acids, Clin Chem, 34: 1077, 1988 – reference: 4) G Hoffmann et al: Quantitative analysis for organic acids in biological samples Batch isolation followed by gas chromatographic-mass spectrometric analysis, Clin Chem, 35: 587, 1989 – reference: 2) J Greter et al: Urinary organic acids: Isolation and quantification for routine metabolic screening, Clin Chem, 33: 473, 1987 – reference: 6) T Nakanishi et al: Quantitative analysis of urinary pyroglutamic acid in hyperammonemia, Clin Chim Acta, 197: 249, 1991 – reference: 1) T Nakanishi et al: Urinary organic acids in elderly Japanese patients with ketosis and encephalopathy who have taken pantoyl-gamma-aminobutyrate, calcium salt (calcium hopantenate, HOPA), Clin Chim Acta, 188: 85, 1990 |
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| Snippet | We describe a quantitative analysis of major urinary organic acids by stable isotope dilution method as a chemical diagnosis of metabolic disorders. In this... |
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| SubjectTerms | gas chromatography/mass spectrometry routine metabolic screening stable isotope dilution method ガスクロマトグラフィー/質量分析(GC/MS) 代謝異常症スクリーニング検査 安定同位体希釈法 |
| Title | QUANTITATIVE ANALYSIS OF URINARY ORGANIC ACIDS BY STABLE ISOTOPE DILUTION METHOD: APPLICATION AS ROUTINE SCREENING TEST FOR METABOLIC DISORDERS |
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