Rapid determination of a stable glycated hemoglobin, s-A1c, by HPLC
Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of the average blood glucose value for the preceding 2 months. Therefore, determination of s-A1c is useful for the assessment of long-term contro...
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Published in | BUNSEKI KAGAKU Vol. 40; no. 8; pp. 395 - 400 |
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Main Authors | , , , |
Format | Journal Article |
Language | Japanese |
Published |
The Japan Society for Analytical Chemistry
05.08.1991
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Subjects | |
Online Access | Get full text |
ISSN | 0525-1931 |
DOI | 10.2116/bunsekikagaku.40.8_395 |
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Abstract | Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of the average blood glucose value for the preceding 2 months. Therefore, determination of s-A1c is useful for the assessment of long-term control of diabetes mellitus. A rapid analysis method of s-A1c by HPLC was discussed. Packings with 5 μm and 3.5 μm diameter polymethacrylate gel modified with carboxymethyl groups, were prepared, and a correlation between particle diameter of the packings and chromatographic performances, such as number of theoretical plates (N), retention time (tR), pressure drop (ΔP) were studied. N increased as the particle diameter of packings was decreased. The N for s-A1c by linear gradient elution was 2× 103 plates for 3.5 μm diameter packing, which was five times larger than that of 5 μm diameter packings. Using 3.5 μm packings, s-A1c was well separated from 1-A1c by the optimization of analytical conditions, such as the concen-tration of the initial eluent. Using the column (4.6 mm i.d. × 35 mm) packed with 3.5 μm packings, six hemoglobin components, A1a, A1b, HbF, 1-A1c, s-A1c and A0, were separated in a 3.5 min cycle by three step gradient elution, thereby reducing analysis time. |
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AbstractList | Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of the average blood glucose value for the preceding 2 months. Therefore, determination of s-A1c is useful for the assessment of long-term control of diabetes mellitus. A rapid analysis method of s-A1c by HPLC was discussed. Packings with 5 μm and 3.5 μm diameter polymethacrylate gel modified with carboxymethyl groups, were prepared, and a correlation between particle diameter of the packings and chromatographic performances, such as number of theoretical plates (N), retention time (tR), pressure drop (ΔP) were studied. N increased as the particle diameter of packings was decreased. The N for s-A1c by linear gradient elution was 2× 103 plates for 3.5 μm diameter packing, which was five times larger than that of 5 μm diameter packings. Using 3.5 μm packings, s-A1c was well separated from 1-A1c by the optimization of analytical conditions, such as the concen-tration of the initial eluent. Using the column (4.6 mm i.d. × 35 mm) packed with 3.5 μm packings, six hemoglobin components, A1a, A1b, HbF, 1-A1c, s-A1c and A0, were separated in a 3.5 min cycle by three step gradient elution, thereby reducing analysis time. |
Author | TOCHIGI, Kenji MIURA, Junkichi MIZUNO, Masako ITO, Masahito |
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References | 5) 日本化学会編 : "新実験講座18, 界面とコロイド", p.123(1977), (丸善). 4) 高橋裕明, 山口昌里, 田中勝之, 村本勝也, 海野益郎 : 日本臨床検査自動化学会会誌, 12,133(1987). 2) 松宮和人 : 検査と技術, 14, 1155(1986). 1) 中川昌一, 中山秀隆 : Glycated hemoglobin, 日本臨床1986夏季増刊, 糖尿病-適正な治療と管理, p.539(1986), (日本臨床社). 3) 加瀬沢信彦, 新里光子, 丸山信幸, 浦田武義, 小林幸子, 島宗良子, 関野千賀子, 酒井由美子, 片桐智美, 伊豆一雄, 荒井奥弘, 金子兼三, 鴨井久司 : 臨床病理, 35,203(1987). 6) 日本化学会編 : "改訂3版化学便覧応用編", p.689, (1980), (丸善). 7) 星野忠夫, 鈴木美貴子, 穴沢典子, 門屋裕子 : 第31回液体クロマトグラフ研究会講演要旨集, p.175(1989). |
References_xml | – reference: 1) 中川昌一, 中山秀隆 : Glycated hemoglobin, 日本臨床1986夏季増刊, 糖尿病-適正な治療と管理, p.539(1986), (日本臨床社). – reference: 7) 星野忠夫, 鈴木美貴子, 穴沢典子, 門屋裕子 : 第31回液体クロマトグラフ研究会講演要旨集, p.175(1989). – reference: 5) 日本化学会編 : "新実験講座18, 界面とコロイド", p.123(1977), (丸善). – reference: 6) 日本化学会編 : "改訂3版化学便覧応用編", p.689, (1980), (丸善). – reference: 3) 加瀬沢信彦, 新里光子, 丸山信幸, 浦田武義, 小林幸子, 島宗良子, 関野千賀子, 酒井由美子, 片桐智美, 伊豆一雄, 荒井奥弘, 金子兼三, 鴨井久司 : 臨床病理, 35,203(1987). – reference: 2) 松宮和人 : 検査と技術, 14, 1155(1986). – reference: 4) 高橋裕明, 山口昌里, 田中勝之, 村本勝也, 海野益郎 : 日本臨床検査自動化学会会誌, 12,133(1987). |
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Snippet | Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of... |
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StartPage | 395 |
SubjectTerms | correlation between particle diameter and chromatographic performance determination of glycated hemoglobins by HPLC measurement of stable A1c |
Title | Rapid determination of a stable glycated hemoglobin, s-A1c, by HPLC |
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