Rapid determination of a stable glycated hemoglobin, s-A1c, by HPLC

Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of the average blood glucose value for the preceding 2 months. Therefore, determination of s-A1c is useful for the assessment of long-term contro...

Full description

Saved in:
Bibliographic Details
Published inBUNSEKI KAGAKU Vol. 40; no. 8; pp. 395 - 400
Main Authors MIZUNO, Masako, MIURA, Junkichi, ITO, Masahito, TOCHIGI, Kenji
Format Journal Article
LanguageJapanese
Published The Japan Society for Analytical Chemistry 05.08.1991
Subjects
Online AccessGet full text
ISSN0525-1931
DOI10.2116/bunsekikagaku.40.8_395

Cover

Abstract Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of the average blood glucose value for the preceding 2 months. Therefore, determination of s-A1c is useful for the assessment of long-term control of diabetes mellitus. A rapid analysis method of s-A1c by HPLC was discussed. Packings with 5 μm and 3.5 μm diameter polymethacrylate gel modified with carboxymethyl groups, were prepared, and a correlation between particle diameter of the packings and chromatographic performances, such as number of theoretical plates (N), retention time (tR), pressure drop (ΔP) were studied. N increased as the particle diameter of packings was decreased. The N for s-A1c by linear gradient elution was 2× 103 plates for 3.5 μm diameter packing, which was five times larger than that of 5 μm diameter packings. Using 3.5 μm packings, s-A1c was well separated from 1-A1c by the optimization of analytical conditions, such as the concen-tration of the initial eluent. Using the column (4.6 mm i.d. × 35 mm) packed with 3.5 μm packings, six hemoglobin components, A1a, A1b, HbF, 1-A1c, s-A1c and A0, were separated in a 3.5 min cycle by three step gradient elution, thereby reducing analysis time.
AbstractList Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of the average blood glucose value for the preceding 2 months. Therefore, determination of s-A1c is useful for the assessment of long-term control of diabetes mellitus. A rapid analysis method of s-A1c by HPLC was discussed. Packings with 5 μm and 3.5 μm diameter polymethacrylate gel modified with carboxymethyl groups, were prepared, and a correlation between particle diameter of the packings and chromatographic performances, such as number of theoretical plates (N), retention time (tR), pressure drop (ΔP) were studied. N increased as the particle diameter of packings was decreased. The N for s-A1c by linear gradient elution was 2× 103 plates for 3.5 μm diameter packing, which was five times larger than that of 5 μm diameter packings. Using 3.5 μm packings, s-A1c was well separated from 1-A1c by the optimization of analytical conditions, such as the concen-tration of the initial eluent. Using the column (4.6 mm i.d. × 35 mm) packed with 3.5 μm packings, six hemoglobin components, A1a, A1b, HbF, 1-A1c, s-A1c and A0, were separated in a 3.5 min cycle by three step gradient elution, thereby reducing analysis time.
Author TOCHIGI, Kenji
MIURA, Junkichi
MIZUNO, Masako
ITO, Masahito
Author_xml – sequence: 1
  fullname: MIZUNO, Masako
  organization: Hitachi Research Laboratory, Hitachi Ltd
– sequence: 1
  fullname: MIURA, Junkichi
  organization: Naka Works, Hitachi Ltd
– sequence: 1
  fullname: ITO, Masahito
  organization: Naka Works, Hitachi Ltd
– sequence: 1
  fullname: TOCHIGI, Kenji
  organization: Hitachi Research Laboratory, Hitachi Ltd
BookMark eNpVUFFLwzAYzMME59xfkPyAdSZfk659HEWdMFFEn8OXL2mXrUtH2z303zuYCL7cwXEcd3fHJrGNnrEHKZYgZfZoz7H3h3DAGg_npRLL3KSFnrCp0KATWaTyls37PlghIAcQoKas_MRTcNz5wXfHEHEIbeRtxZH3A9rG87oZCQfv-M4f27ppbYgL3idrSQtuR7752Jb37KbCpvfzX56x7-enr3KTbN9fXsv1NtmDhCEBh1VBMrUKQbqLlCmnaIUkM11Vwmc6BZAFKIVkEbJcO0srZZFIEDmXztjbNXd_6VZ7c-rCEbvRYDcEarz5t18WGowSJr_C5Yg_H-2wMz6mP43nYFY
ContentType Journal Article
Copyright The Japan Society for Analytical Chemistry
Copyright_xml – notice: The Japan Society for Analytical Chemistry
DOI 10.2116/bunsekikagaku.40.8_395
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EndPage 400
ExternalDocumentID article_bunsekikagaku1952_40_8_40_8_395_article_char_en
GroupedDBID 53G
5GY
6J9
ACIWK
AENEX
ALMA_UNASSIGNED_HOLDINGS
CS3
DU5
EBS
EJD
HH5
JSF
KQ8
OK1
P2P
RJT
~02
ID FETCH-LOGICAL-j212t-2daf9c13b4a21d21264d4c7ac165ff0e6532219244acba2685dbc74bacc0ccdd3
ISSN 0525-1931
IngestDate Wed Sep 03 06:22:36 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 8
Language Japanese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-j212t-2daf9c13b4a21d21264d4c7ac165ff0e6532219244acba2685dbc74bacc0ccdd3
OpenAccessLink https://www.jstage.jst.go.jp/article/bunsekikagaku1952/40/8/40_8_395/_article/-char/en
PageCount 6
ParticipantIDs jstage_primary_article_bunsekikagaku1952_40_8_40_8_395_article_char_en
PublicationCentury 1900
PublicationDate 1991/08/05
PublicationDateYYYYMMDD 1991-08-05
PublicationDate_xml – month: 08
  year: 1991
  text: 1991/08/05
  day: 05
PublicationDecade 1990
PublicationTitle BUNSEKI KAGAKU
PublicationTitleAlternate BUNSEKI KAGAKU
PublicationYear 1991
Publisher The Japan Society for Analytical Chemistry
Publisher_xml – name: The Japan Society for Analytical Chemistry
References 5) 日本化学会編 : "新実験講座18, 界面とコロイド", p.123(1977), (丸善).
4) 高橋裕明, 山口昌里, 田中勝之, 村本勝也, 海野益郎 : 日本臨床検査自動化学会会誌, 12,133(1987).
2) 松宮和人 : 検査と技術, 14, 1155(1986).
1) 中川昌一, 中山秀隆 : Glycated hemoglobin, 日本臨床1986夏季増刊, 糖尿病-適正な治療と管理, p.539(1986), (日本臨床社).
3) 加瀬沢信彦, 新里光子, 丸山信幸, 浦田武義, 小林幸子, 島宗良子, 関野千賀子, 酒井由美子, 片桐智美, 伊豆一雄, 荒井奥弘, 金子兼三, 鴨井久司 : 臨床病理, 35,203(1987).
6) 日本化学会編 : "改訂3版化学便覧応用編", p.689, (1980), (丸善).
7) 星野忠夫, 鈴木美貴子, 穴沢典子, 門屋裕子 : 第31回液体クロマトグラフ研究会講演要旨集, p.175(1989).
References_xml – reference: 1) 中川昌一, 中山秀隆 : Glycated hemoglobin, 日本臨床1986夏季増刊, 糖尿病-適正な治療と管理, p.539(1986), (日本臨床社).
– reference: 7) 星野忠夫, 鈴木美貴子, 穴沢典子, 門屋裕子 : 第31回液体クロマトグラフ研究会講演要旨集, p.175(1989).
– reference: 5) 日本化学会編 : "新実験講座18, 界面とコロイド", p.123(1977), (丸善).
– reference: 6) 日本化学会編 : "改訂3版化学便覧応用編", p.689, (1980), (丸善).
– reference: 3) 加瀬沢信彦, 新里光子, 丸山信幸, 浦田武義, 小林幸子, 島宗良子, 関野千賀子, 酒井由美子, 片桐智美, 伊豆一雄, 荒井奥弘, 金子兼三, 鴨井久司 : 臨床病理, 35,203(1987).
– reference: 2) 松宮和人 : 検査と技術, 14, 1155(1986).
– reference: 4) 高橋裕明, 山口昌里, 田中勝之, 村本勝也, 海野益郎 : 日本臨床検査自動化学会会誌, 12,133(1987).
SSID ssib002822024
ssib000936166
ssib017172177
ssib023161072
ssj0025051
ssib002670215
ssib003171134
Score 1.3996918
Snippet Glycated hemoglobins (GHb) are composed of A1a, A1b, labile-A1c (1-A1c), stable-A1c (s-A1c) and others. The concentration of s-A1c can be used as an index of...
SourceID jstage
SourceType Publisher
StartPage 395
SubjectTerms correlation between particle diameter and chromatographic performance
determination of glycated hemoglobins by HPLC
measurement of stable A1c
Title Rapid determination of a stable glycated hemoglobin, s-A1c, by HPLC
URI https://www.jstage.jst.go.jp/article/bunsekikagaku1952/40/8/40_8_395/_article/-char/en
Volume 40
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
ispartofPNX BUNSEKI KAGAKU, 1991/08/05, Vol.40(8), pp.395-400
journalDatabaseRights – providerCode: PRVFSB
  databaseName: Free Full-Text Journals in Chemistry
  issn: 0525-1931
  databaseCode: HH5
  dateStart: 19520101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: http://abc-chemistry.org/
  omitProxy: true
  ssIdentifier: ssj0025051
  providerName: ABC ChemistRy
– providerCode: PRVAFT
  databaseName: Open Access Digital Library
  issn: 0525-1931
  databaseCode: KQ8
  dateStart: 19520101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html
  omitProxy: true
  ssIdentifier: ssj0025051
  providerName: Colorado Alliance of Research Libraries
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3JbtswECXc9NBegq5ouoGH3hK5okRR0lEwktg16rSpBQS9CENSipfGDhr70P5Kf7ZDUZvRHNqgF8IiRAH0PA0fR3wzhLzzYl3wnCknh1w7nGvtQMjAKWIptZ8zpgsjcP44EcOUf7gILnq9X51TS9uN7Kuft-pK7mJV7EO7GpXsP1i2eSh24G-0L7ZoYWz_ysbncD3Xh7o-0VKTPzABAqOIuvxmwjNIKWf51dqk_rAJA26chJWFzZF6Dj9VVYPrD7vp5MvxeHQ4Tk6TcdqYY_Q1nZxZbc8NLNdtf3qeWHHHajlXs3mDtGlz9wx9RhMfOBsMR6ejShC0mLcxB3s2KnLcoEFJqVrDtXy1c7S0zKJiA_CDulhdx5sFXuAgW2Rd12szNVUQizp-1LeVN6slmZfJTP_w9rh3NYEHabT7y_kSLmG57XO3H2XN8J1M2pWdsp0BLA68jLtZZBscmdX3Gd0bwuweue-FQpjyGOPPHe4a-4J1974iNPypu5f13E4qfCRqjLW5-PAKN9-tCBh5NjLZsMl9ZqhpWe6x_t-svN3M-f3tM0YOtUB41acRS4I0fUT2q50NTey0HpPeAp6QB42NnpJBCVe6A1e6LihQC1daw5W2cD2iJViPqPxBDVSfkfTkeDoYOlUND2eBpGjjeBqKWDFfcvCYxi7BNVchKCaConBzEeCKYoIAHJQET0SBlirkEpRyldLaf072VutV_oJQQLfiSx26UOQ85B4U4EMQxlIgK41BHJATO__s2iZqye5o8Jf_60GvyMP27XlN9jbft_kb5LAb-bbE0m_wepnN
linkProvider Colorado Alliance of Research Libraries
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Rapid+determination+of+a+stable+glycated+hemoglobin%2C+s-A1c%2C+by+HPLC&rft.jtitle=BUNSEKI+KAGAKU&rft.au=MIZUNO%2C+Masako&rft.au=MIURA%2C+Junkichi&rft.au=ITO%2C+Masahito&rft.au=TOCHIGI%2C+Kenji&rft.date=1991-08-05&rft.pub=The+Japan+Society+for+Analytical+Chemistry&rft.issn=0525-1931&rft.volume=40&rft.issue=8&rft.spage=395&rft.epage=400&rft_id=info:doi/10.2116%2Fbunsekikagaku.40.8_395&rft.externalDocID=article_bunsekikagaku1952_40_8_40_8_395_article_char_en
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0525-1931&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0525-1931&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0525-1931&client=summon