ヒト好酸球および好中球の活性酸素種産生の比較検討-とくにアレルギーにおける好酸球活性酸素の意義について
正常者における好酸球の活性酸素産生能に関して細胞外,細胞内化学発光, O2-産生, H2O2産生を測定して好中球と比較した.またアレルギー患者と非アレルギー者および気管支喘息患者の発作時と非発作時の好酸球の化学発光を比較し,アレルギー疾患における好酸球の活性酸素産生について考察した. 正常者における活性酸素産生はPMA刺激では好酸球のほうが好中球より高値であった.アレルギー患者と非アレルギー者の好酸球の比較では,両者の化学発光に明らかな差はなかった.気管支喘息患者の発作時好酸球は非発作時よりも高い化学発光を示し,発作時好中球も非発作時より高い化学発光を示した.好酸球は刺激物質の種類によっては好...
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| Published in | 日本臨床免疫学会会誌 Vol. 14; no. 2; pp. 166 - 173 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Japanese |
| Published |
日本臨床免疫学会
1991
|
| Online Access | Get full text |
| ISSN | 0911-4300 1349-7413 |
| DOI | 10.2177/jsci.14.166 |
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| Abstract | 正常者における好酸球の活性酸素産生能に関して細胞外,細胞内化学発光, O2-産生, H2O2産生を測定して好中球と比較した.またアレルギー患者と非アレルギー者および気管支喘息患者の発作時と非発作時の好酸球の化学発光を比較し,アレルギー疾患における好酸球の活性酸素産生について考察した. 正常者における活性酸素産生はPMA刺激では好酸球のほうが好中球より高値であった.アレルギー患者と非アレルギー者の好酸球の比較では,両者の化学発光に明らかな差はなかった.気管支喘息患者の発作時好酸球は非発作時よりも高い化学発光を示し,発作時好中球も非発作時より高い化学発光を示した.好酸球は刺激物質の種類によっては好中球より多量の活性酸素を産生する能力を有しており,喘息発作時には好酸球,好中球の産生する活性酸素が組織傷害の一因となっている可能性が示唆された. |
|---|---|
| AbstractList | 正常者における好酸球の活性酸素産生能に関して細胞外,細胞内化学発光, O2-産生, H2O2産生を測定して好中球と比較した.またアレルギー患者と非アレルギー者および気管支喘息患者の発作時と非発作時の好酸球の化学発光を比較し,アレルギー疾患における好酸球の活性酸素産生について考察した. 正常者における活性酸素産生はPMA刺激では好酸球のほうが好中球より高値であった.アレルギー患者と非アレルギー者の好酸球の比較では,両者の化学発光に明らかな差はなかった.気管支喘息患者の発作時好酸球は非発作時よりも高い化学発光を示し,発作時好中球も非発作時より高い化学発光を示した.好酸球は刺激物質の種類によっては好中球より多量の活性酸素を産生する能力を有しており,喘息発作時には好酸球,好中球の産生する活性酸素が組織傷害の一因となっている可能性が示唆された. |
| Author | 吉国, 裕文 |
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| References | 13) Shult, P. A., Graziano, F. M., Wallow, I. M. et al.: Comparison of superoxide generation and luminol-dependent chemiluminescence with eosinophils and neutrophils from normal individuals. J. Lab. Clin. Med., 106: 638-645, 1985. 17) Shult, P. A., Graziano, F. M., Busse, W. W.: Enhanced eosinophil luminol-dependent chemiluminescence in allergic rhinitis. J. Allergy Clin. Immunol., 77: 702-708, 1986. 1) Barker, R. L., Loegering, D. A., Ten, R. M. et al.: Eosinophil cationic protein cDNA. J. Immunol., 143: 952-955, 1989. 4) 吉国裕文・林 克敏・辻 芳郎:好酸球の単離,臨床免疫, 22: 22～27, 1990. 6) Fukuda, T., Dunette, S. L., Charles, E. R. et al.: Increased numbers of hypodense eosinophils in the blood of patients with bronchial asthma. Am. Rev. Respir. Dis., 132: 981-985, 1985. 8) Allen, R. C., Loose, L. D.: Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochem. Biophys. Res. Commun., 69: 245-252, 1976. 5) Gärtner, I.: Separation of human eosinophils in density gradients of polyvinylpyrrolidone coated silica gel (Percoll). Immunology, 40: 133-136, 1980. 9) Johnston, R. B. Jr., Keele, B. B. Jr., Misra, H. P. et al.: The role of superoxide anion generation in phagocytic bactericidal activity: Studies with normal and chronic granulomatous disease leukocytes. J. Clin. Invest., 55: 1357-1372, 1975. 15) Kloprogge, E., de Leeuw, A. J., de Monchy, J. G. R. et al.: Hypodense eosinophilic granulocytes in normal individuals and patients with asthma: Generation of hypodense cell popurations in vitro. J. Allergy Clin. Immunol., 83: 393-400, 1989. 18) Hakansson, L., Rak, S., Dahl, R. et al.: The formation of eosinophil and neutrophil chemotactic activity during a pollen season and after allergen challenge. J. Allergy Clin. Immunol., 83: 933-939, 1989. 2) Learn, D. B., Brestel, E. P.: A comparison of superoxide production by human eosinophils and neutrophils. Agents and Actions, 12: 485-488, 1982. 10) Pick, E., Keisari, Y.: A simple colorimetric method for the measurment of hydrogen peroxide production by cells in culture. J. Immunol. Methods., 38: 161-170, 1980. 11) Shult, P. A., Lega, M., Jadidi, S. et al.: The presence of hypodense eosinophils and diminished chemiluminescence response in asthma. J. Allergy Clin. Immunol., 81: 429-437, 1988. 14) Yazdanbakhsh, M., Eckmann, C. M., de Boer, M. et al.: Purification of eosinophils from normal human blood, preparation of eosinoplasts and characterization of their functional response to various stimuli. Immunology, 60: 123-129, 1987. 19) Sedgwick, J. B., Geiger, K. M., Busse, W. W.: Superoxide generation by hypodense eosinophils from patients with asthma. Am. Rev. Respir. Dis., 142: 120-125, 1990. 20) Pincus, S. H., Schooley, W. R., DiNapoli, A. M. et al.: Metabolic heterogeneity of eosinophils from normal and hypereosinophilic patients. Blood, 58: 1175-1181, 1981. 3) Petreccia, D. C., Nauseef, W. M., Clark, R. A.: Respiratory burst of normal human eosinophils. J. Leukocyte Biol., 41: 283-288, 1987. 7) 辻 芳郎:白血球機能検査.臨床検査マニュアル,北村元士・三輪史朗・三輪谷俊夫・他編,第1版,文光堂,東京, 1988, pp. 452～462. 12) Prin, L., Charon, J., Capron, M. et al.: Heterogeneity of human eosinophils. II. Variability of respiratory burst activity related to cell density. Clin. Exp. Immunol., 57: 735-742, 1984. 16) Rowen, J. L., Hyde, D. M., Mcdonald, R. J.: Eosinophils cause acute edematous injury in isorated perfused rat lungs. Am. Rev. Respir. Dis., 142: 215-220, 1990. |
| References_xml | – reference: 20) Pincus, S. H., Schooley, W. R., DiNapoli, A. M. et al.: Metabolic heterogeneity of eosinophils from normal and hypereosinophilic patients. Blood, 58: 1175-1181, 1981. – reference: 6) Fukuda, T., Dunette, S. L., Charles, E. R. et al.: Increased numbers of hypodense eosinophils in the blood of patients with bronchial asthma. Am. Rev. Respir. Dis., 132: 981-985, 1985. – reference: 3) Petreccia, D. C., Nauseef, W. M., Clark, R. A.: Respiratory burst of normal human eosinophils. J. Leukocyte Biol., 41: 283-288, 1987. – reference: 7) 辻 芳郎:白血球機能検査.臨床検査マニュアル,北村元士・三輪史朗・三輪谷俊夫・他編,第1版,文光堂,東京, 1988, pp. 452～462. – reference: 19) Sedgwick, J. B., Geiger, K. M., Busse, W. W.: Superoxide generation by hypodense eosinophils from patients with asthma. Am. Rev. Respir. Dis., 142: 120-125, 1990. – reference: 13) Shult, P. A., Graziano, F. M., Wallow, I. M. et al.: Comparison of superoxide generation and luminol-dependent chemiluminescence with eosinophils and neutrophils from normal individuals. J. Lab. Clin. Med., 106: 638-645, 1985. – reference: 10) Pick, E., Keisari, Y.: A simple colorimetric method for the measurment of hydrogen peroxide production by cells in culture. J. Immunol. Methods., 38: 161-170, 1980. – reference: 2) Learn, D. B., Brestel, E. P.: A comparison of superoxide production by human eosinophils and neutrophils. Agents and Actions, 12: 485-488, 1982. – reference: 16) Rowen, J. L., Hyde, D. M., Mcdonald, R. J.: Eosinophils cause acute edematous injury in isorated perfused rat lungs. Am. Rev. Respir. Dis., 142: 215-220, 1990. – reference: 1) Barker, R. L., Loegering, D. A., Ten, R. M. et al.: Eosinophil cationic protein cDNA. J. Immunol., 143: 952-955, 1989. – reference: 11) Shult, P. A., Lega, M., Jadidi, S. et al.: The presence of hypodense eosinophils and diminished chemiluminescence response in asthma. J. Allergy Clin. Immunol., 81: 429-437, 1988. – reference: 15) Kloprogge, E., de Leeuw, A. J., de Monchy, J. G. R. et al.: Hypodense eosinophilic granulocytes in normal individuals and patients with asthma: Generation of hypodense cell popurations in vitro. J. Allergy Clin. Immunol., 83: 393-400, 1989. – reference: 8) Allen, R. C., Loose, L. D.: Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochem. Biophys. Res. Commun., 69: 245-252, 1976. – reference: 12) Prin, L., Charon, J., Capron, M. et al.: Heterogeneity of human eosinophils. II. Variability of respiratory burst activity related to cell density. Clin. Exp. Immunol., 57: 735-742, 1984. – reference: 9) Johnston, R. B. Jr., Keele, B. B. Jr., Misra, H. P. et al.: The role of superoxide anion generation in phagocytic bactericidal activity: Studies with normal and chronic granulomatous disease leukocytes. J. Clin. Invest., 55: 1357-1372, 1975. – reference: 18) Hakansson, L., Rak, S., Dahl, R. et al.: The formation of eosinophil and neutrophil chemotactic activity during a pollen season and after allergen challenge. J. Allergy Clin. Immunol., 83: 933-939, 1989. – reference: 5) Gärtner, I.: Separation of human eosinophils in density gradients of polyvinylpyrrolidone coated silica gel (Percoll). Immunology, 40: 133-136, 1980. – reference: 4) 吉国裕文・林 克敏・辻 芳郎:好酸球の単離,臨床免疫, 22: 22～27, 1990. – reference: 14) Yazdanbakhsh, M., Eckmann, C. M., de Boer, M. et al.: Purification of eosinophils from normal human blood, preparation of eosinoplasts and characterization of their functional response to various stimuli. Immunology, 60: 123-129, 1987. – reference: 17) Shult, P. A., Graziano, F. M., Busse, W. W.: Enhanced eosinophil luminol-dependent chemiluminescence in allergic rhinitis. J. Allergy Clin. Immunol., 77: 702-708, 1986. |
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| Title | ヒト好酸球および好中球の活性酸素種産生の比較検討-とくにアレルギーにおける好酸球活性酸素の意義について |
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