質量分析法（VITEK MS）と生化学的性状による臨床分離株同定の比較検討
マトリックス支援レーザー脱離・イオン化―飛行時間型質量分析（MALDI-TOF MS）法による細菌名同定に関する報告は多数あるが，最新のMALDI-TOF MS装置であるVITEK MSと従来の細菌名同定法を比較した報告は少ない．我々は臨床分離株314株を用いて，VITEK MSと細菌の生化学的性状に基づく菌名同定機器VITEK2の菌名同定結果を比較した．VITEK MSの同定率は属レベルで93.6%，種レベルで88.9%，VITEK2の同定率は属レベルで95.9%，種レベルで85.0%であった．属レベルではVITEK2の方がVITEK MSより高い同定率を示したが，種レベルではVITEK M...
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| Published in | 医学検査 Vol. 63; no. 5; pp. 573 - 578 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
一般社団法人 日本臨床衛生検査技師会
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0915-8669 2188-5346 |
| DOI | 10.14932/jamt.13-91 |
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| Abstract | マトリックス支援レーザー脱離・イオン化―飛行時間型質量分析（MALDI-TOF MS）法による細菌名同定に関する報告は多数あるが，最新のMALDI-TOF MS装置であるVITEK MSと従来の細菌名同定法を比較した報告は少ない．我々は臨床分離株314株を用いて，VITEK MSと細菌の生化学的性状に基づく菌名同定機器VITEK2の菌名同定結果を比較した．VITEK MSの同定率は属レベルで93.6%，種レベルで88.9%，VITEK2の同定率は属レベルで95.9%，種レベルで85.0%であった．属レベルではVITEK2の方がVITEK MSより高い同定率を示したが，種レベルではVITEK MSがVITEK2より有意に高い同定率を示した．腸内細菌科の株では検査手技に起因してVITEK MSで菌種が同定できないことがあった．手技の向上及びデータベースの整備によりVITEK MSの同定率は上がると考えられる． |
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| AbstractList | マトリックス支援レーザー脱離・イオン化―飛行時間型質量分析（MALDI-TOF MS）法による細菌名同定に関する報告は多数あるが，最新のMALDI-TOF MS装置であるVITEK MSと従来の細菌名同定法を比較した報告は少ない．我々は臨床分離株314株を用いて，VITEK MSと細菌の生化学的性状に基づく菌名同定機器VITEK2の菌名同定結果を比較した．VITEK MSの同定率は属レベルで93.6%，種レベルで88.9%，VITEK2の同定率は属レベルで95.9%，種レベルで85.0%であった．属レベルではVITEK2の方がVITEK MSより高い同定率を示したが，種レベルではVITEK MSがVITEK2より有意に高い同定率を示した．腸内細菌科の株では検査手技に起因してVITEK MSで菌種が同定できないことがあった．手技の向上及びデータベースの整備によりVITEK MSの同定率は上がると考えられる． |
| Author | 服部, 拓哉 池上, 志乃富 山岸, 宏江 鈴木, 真由子 湯浅, 典博 村上, いつか 西山, 秀樹 美濃島, 慎 |
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| References | 5) Sauer S, Kliem M : Mass spectrometry tools for the classification and identification of bacteria, Nat Rev Microbiol 2010 ; 8 : 74–82. 7) Neville SA et al. : Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification, J Clin Microbiol 2011 ; 49 : 2980–2984. 3) Karas M, Hillenkamp F : Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons, Anal Chem 1988 ; 60 : 2299–2301. 6) Cherkaoui A et al. : Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level, J Clin Microbiol 2010 ; 48 : 1169–1175. 4) Seng P et al. : Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Clin Infect Dis 2009 ; 49 : 543–551. 9) Bizzini A et al. : Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory, J Clin Microbiol 2010 ; 48 : 1549–1554. 10) Dhiman N et al. : Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast, J Clin Microbiol 2011 ; 49 : 1614–1616. 1) Saffert RT et al. : Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli, J Clin Microbiol 2011 ; 49 : 887–892. 8) Dubois D et al. : Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology, J Clin Microbiol 2012 ; 50 : 2568–2576. 2) Risch M et al. : Comparison of MALDI TOF with conventional identification of clinically relevant bacteria, Swiss Med Wkly 2010 ; 140 : w13095. |
| References_xml | – reference: 5) Sauer S, Kliem M : Mass spectrometry tools for the classification and identification of bacteria, Nat Rev Microbiol 2010 ; 8 : 74–82. – reference: 3) Karas M, Hillenkamp F : Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons, Anal Chem 1988 ; 60 : 2299–2301. – reference: 6) Cherkaoui A et al. : Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level, J Clin Microbiol 2010 ; 48 : 1169–1175. – reference: 8) Dubois D et al. : Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology, J Clin Microbiol 2012 ; 50 : 2568–2576. – reference: 10) Dhiman N et al. : Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast, J Clin Microbiol 2011 ; 49 : 1614–1616. – reference: 1) Saffert RT et al. : Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli, J Clin Microbiol 2011 ; 49 : 887–892. – reference: 9) Bizzini A et al. : Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory, J Clin Microbiol 2010 ; 48 : 1549–1554. – reference: 2) Risch M et al. : Comparison of MALDI TOF with conventional identification of clinically relevant bacteria, Swiss Med Wkly 2010 ; 140 : w13095. – reference: 4) Seng P et al. : Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Clin Infect Dis 2009 ; 49 : 543–551. – reference: 7) Neville SA et al. : Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification, J Clin Microbiol 2011 ; 49 : 2980–2984. |
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| Title | 質量分析法（VITEK MS）と生化学的性状による臨床分離株同定の比較検討 |
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