Studies on cell-mediated immunity (CMI) in the glomeruli: immunization with GBM antigen from Masugi nephritis in Wistar-Kyoto rats
The role of cell-mediated immunity in glomerular injury has been extensively studied, but the precise mechanism remains obscure. This study was undertaken to determine whether the homologous glomerular basement membrane (GBM) combined heterogeneous anti-GBM antibody would induce cell-mediated immuni...
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| Published in | Nihon Jinzo Gakkai shi Vol. 36; no. 2; pp. 95 - 102 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan
Japanese Society of Nephrology
01.02.1994
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0385-2385 1884-0728 |
| DOI | 10.14842/jpnjnephrol1959.36.95 |
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| Abstract | The role of cell-mediated immunity in glomerular injury has been extensively studied, but the precise mechanism remains obscure. This study was undertaken to determine whether the homologous glomerular basement membrane (GBM) combined heterogeneous anti-GBM antibody would induce cell-mediated immunity . Wistar-Kyoto rats were divided into three groups; group I rats were immunized with homologous GBM antigen that was derived from Masugi nephritis and emulsified in complete Freund's adjuvant (CFA); group II rats were immunized with homologous GBM antigen that was derived from normal rats and emulsified in CFA; group Ill rats were immunized with CFA only . Group I rats demonstrated proteinuria on days 7 and 10 after immunization. Histologically, mesangial cell proliferation and cell infiltration were observed in the glomeruli . No changes in tubulointerstitial tissue. CD4-positive cells, CD8-positive cells and macrophages were found in the glomeruli, and mononucleor cells were found in the glomerular capillary lumen . Group II and Group IQ rats demonstrated no proteinuria and no histological changes. These findings indicated that the altered GBM obtained antigenicity and induced cell-mediated immunity in the glomeruli . |
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| AbstractList | The role of cell-mediated immunity in glomerular injury has been extensively studied, but the precise mechanism remains obscure. This study was undertaken to determine whether the homologous glomerular basement membrane (GBM) combined heterogeneous anti-GBM antibody would induce cell-mediated immunity . Wistar-Kyoto rats were divided into three groups; group I rats were immunized with homologous GBM antigen that was derived from Masugi nephritis and emulsified in complete Freund's adjuvant (CFA); group II rats were immunized with homologous GBM antigen that was derived from normal rats and emulsified in CFA; group Ill rats were immunized with CFA only . Group I rats demonstrated proteinuria on days 7 and 10 after immunization. Histologically, mesangial cell proliferation and cell infiltration were observed in the glomeruli . No changes in tubulointerstitial tissue. CD4-positive cells, CD8-positive cells and macrophages were found in the glomeruli, and mononucleor cells were found in the glomerular capillary lumen . Group II and Group IQ rats demonstrated no proteinuria and no histological changes. These findings indicated that the altered GBM obtained antigenicity and induced cell-mediated immunity in the glomeruli . The role of cell-mediated immunity in glomerular injury has been extensively studied, but the precise mechanism remains obscure. This study was undertaken to determine whether the homologous glomerular basement membrane (GBM) combined heterogeneous anti-GBM antibody would induce cell-mediated immunity. Wistar-Kyoto rats were divided into three groups; group I rats were immunized with homologous GBM antigen that was derived from Masugi nephritis and emulsified in complete Freund's adjuvant (CFA); group II rats were immunized with homologous GBM antigen that was derived from normal rats and emulsified in CFA; group III rats were immunized with CFA only. Group I rats demonstrated proteinuria on days 7 and 10 after immunization. Histologically, mesangial cell proliferation and cell infiltration were observed in the glomeruli. No changes in tubulointerstitial tissue. CD4-positive cells, CD8-positive cells and macrophages were found in the glomeruli, and mononuclear cells were found in the glomerular capillary lumen. Group II and Group III rats demonstrated no proteinuria and no histological changes. These findings indicated that the altered GBM obtained antigenicity and induced cell-mediated immunity in the glomeruli.The role of cell-mediated immunity in glomerular injury has been extensively studied, but the precise mechanism remains obscure. This study was undertaken to determine whether the homologous glomerular basement membrane (GBM) combined heterogeneous anti-GBM antibody would induce cell-mediated immunity. Wistar-Kyoto rats were divided into three groups; group I rats were immunized with homologous GBM antigen that was derived from Masugi nephritis and emulsified in complete Freund's adjuvant (CFA); group II rats were immunized with homologous GBM antigen that was derived from normal rats and emulsified in CFA; group III rats were immunized with CFA only. Group I rats demonstrated proteinuria on days 7 and 10 after immunization. Histologically, mesangial cell proliferation and cell infiltration were observed in the glomeruli. No changes in tubulointerstitial tissue. CD4-positive cells, CD8-positive cells and macrophages were found in the glomeruli, and mononuclear cells were found in the glomerular capillary lumen. Group II and Group III rats demonstrated no proteinuria and no histological changes. These findings indicated that the altered GBM obtained antigenicity and induced cell-mediated immunity in the glomeruli. The role of cell-mediated immunity in glomerular injury has been extensively studied, but the precise mechanism remains obscure. This study was undertaken to determine whether the homologous glomerular basement membrane (GBM) combined heterogeneous anti-GBM antibody would induce cell-mediated immunity. Wistar-Kyoto rats were divided into three groups; group I rats were immunized with homologous GBM antigen that was derived from Masugi nephritis and emulsified in complete Freund's adjuvant (CFA); group II rats were immunized with homologous GBM antigen that was derived from normal rats and emulsified in CFA; group III rats were immunized with CFA only. Group I rats demonstrated proteinuria on days 7 and 10 after immunization. Histologically, mesangial cell proliferation and cell infiltration were observed in the glomeruli. No changes in tubulointerstitial tissue. CD4-positive cells, CD8-positive cells and macrophages were found in the glomeruli, and mononuclear cells were found in the glomerular capillary lumen. Group II and Group III rats demonstrated no proteinuria and no histological changes. These findings indicated that the altered GBM obtained antigenicity and induced cell-mediated immunity in the glomeruli. |
| Author | KITAZAWA, KOZO OKUYAMA, KAZUO SAITO, KENICHI TSUJI, YUICHIRO KOBAYASHI, KENJI SHIBATA, TAKANORI SUGISAKI, TETSUZO |
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| References | 5) Sado Y, Naito I, Akita M, Okigaki T: Strain specific responeses of inbred rats on the severity of experimental autoimmune glomerulonephritis, J Clin Lab Immunol 19: 193-199, 1988 11) Neale TJ, Tipping PG, Carson SD, Holdsworth SR: Participation of cell-mediated immunity in deposition of fibrin in glomerulonephritis, Lancet 20: 421-424, 1988 6) Bhan AK, Collins AB, Schneeberger EE, Mccluskey RT: A cell-mediated reaction against glomerular-bound immune complexes, J Exp Med 150: 1410-1420, 1979 10) Markovic-Lipkovski J, Muller CA, Risler T, Bohle A, Muller GA: Mononuclear Leukocytes, Expression of HLA Class II Antigens and Intercellular Adhesion Molecule 1 in Focal Segmental Glomeru losclerosis, Nephron 59: 286-293, 1991 4) Granados R, Mendrick DL, Rennke HG: Antibodyinduced crescent formation in WKY rats: Potential role of antibody-dependent cell cytotoxicity (ADCC) in vivo. (abst). Kidney Int 37: 414, 1990 9) Nolasco FEB, Cameron JS, Hartley B, Coelho A, Hildreth G, Reuben R: Intraglomerular T cells and monocytes in nephritis: Study with monoclonal antibodies, Kidney Int 31: 1160-1166, 1987 3) Sado Y, Kagawa M, Naito I, Okigaki T: Properties of bovine nephritogenic antigen that induces antiGBM nephritis in rats and its similarity to the Goodpasture antigen, Virchows Archiv B Cell Pathol60:345-351, 1991 7) Hooke DH, Gee DC, Atkins RC: Leukocyte analysis using monoclonal antibodies in human glomerulonephritis, Kidney Int 31: 964-972, 1987 12) Kawasaki K, Yaoita E, Yamamoto T, Kihara I : Depletion of CD 8 positive cells in nephrotoxic serum nephritis of WKY rats, Kidney Int 41: 1517-1526, 1992 13) Holdsworth SR: Fc dependence of macrophage accumulation and subsequent injury in experimental glomerulonephritis, J Immunol 130: 735-739, 1983 14) Lavelle KJ, Durland BD, Yum MN: The effect of antimacrophage antiserum on immune complex glomerulonephritis, J Lab Clin Med 98: 195-205, 1981 8) Bolton WK, Jr. DJ Innes, Sturgill BC, Kaiser DL: T-cells and macrophages in rapidly progressive glomerulonephritis: Clinlcopathologic correlations, Kidney Int 32: 869-876, 1987 1) Spiro RG: Studies on the Renal Glomerular Basement Membrane. Preparation and Chemical Composition, J Biol Chem 242: 1915-1922, 1967 2) Holdsworth SR, Tipping PG: Cell-mediated Immunity in Glomerulonephritis, Immunology of Renal Diseases. In: Immunology Medicine Vol. 16; ed by Pusey CD, Kluwer Academic Publishers, Dordrecht, pp97-122, 1991 |
| References_xml | – reference: 6) Bhan AK, Collins AB, Schneeberger EE, Mccluskey RT: A cell-mediated reaction against glomerular-bound immune complexes, J Exp Med 150: 1410-1420, 1979 – reference: 12) Kawasaki K, Yaoita E, Yamamoto T, Kihara I : Depletion of CD 8 positive cells in nephrotoxic serum nephritis of WKY rats, Kidney Int 41: 1517-1526, 1992 – reference: 1) Spiro RG: Studies on the Renal Glomerular Basement Membrane. Preparation and Chemical Composition, J Biol Chem 242: 1915-1922, 1967 – reference: 4) Granados R, Mendrick DL, Rennke HG: Antibodyinduced crescent formation in WKY rats: Potential role of antibody-dependent cell cytotoxicity (ADCC) in vivo. (abst). Kidney Int 37: 414, 1990 – reference: 2) Holdsworth SR, Tipping PG: Cell-mediated Immunity in Glomerulonephritis, Immunology of Renal Diseases. In: Immunology Medicine Vol. 16; ed by Pusey CD, Kluwer Academic Publishers, Dordrecht, pp97-122, 1991 – reference: 3) Sado Y, Kagawa M, Naito I, Okigaki T: Properties of bovine nephritogenic antigen that induces antiGBM nephritis in rats and its similarity to the Goodpasture antigen, Virchows Archiv B Cell Pathol60:345-351, 1991 – reference: 5) Sado Y, Naito I, Akita M, Okigaki T: Strain specific responeses of inbred rats on the severity of experimental autoimmune glomerulonephritis, J Clin Lab Immunol 19: 193-199, 1988 – reference: 8) Bolton WK, Jr. DJ Innes, Sturgill BC, Kaiser DL: T-cells and macrophages in rapidly progressive glomerulonephritis: Clinlcopathologic correlations, Kidney Int 32: 869-876, 1987 – reference: 13) Holdsworth SR: Fc dependence of macrophage accumulation and subsequent injury in experimental glomerulonephritis, J Immunol 130: 735-739, 1983 – reference: 7) Hooke DH, Gee DC, Atkins RC: Leukocyte analysis using monoclonal antibodies in human glomerulonephritis, Kidney Int 31: 964-972, 1987 – reference: 11) Neale TJ, Tipping PG, Carson SD, Holdsworth SR: Participation of cell-mediated immunity in deposition of fibrin in glomerulonephritis, Lancet 20: 421-424, 1988 – reference: 14) Lavelle KJ, Durland BD, Yum MN: The effect of antimacrophage antiserum on immune complex glomerulonephritis, J Lab Clin Med 98: 195-205, 1981 – reference: 10) Markovic-Lipkovski J, Muller CA, Risler T, Bohle A, Muller GA: Mononuclear Leukocytes, Expression of HLA Class II Antigens and Intercellular Adhesion Molecule 1 in Focal Segmental Glomeru losclerosis, Nephron 59: 286-293, 1991 – reference: 9) Nolasco FEB, Cameron JS, Hartley B, Coelho A, Hildreth G, Reuben R: Intraglomerular T cells and monocytes in nephritis: Study with monoclonal antibodies, Kidney Int 31: 1160-1166, 1987 |
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| SubjectTerms | Animals Basement Membrane - immunology Female Glomerulonephritis - immunology Heymann Nephritis Antigenic Complex Immunity, Cellular Immunization Kidney Glomerulus - immunology mediated immunity, Masugi nephritis, cell infiltration, CD4, CD8 positive cells Membrane Glycoproteins - immunology Rats Rats, Inbred WKY |
| Title | Studies on cell-mediated immunity (CMI) in the glomeruli: immunization with GBM antigen from Masugi nephritis in Wistar-Kyoto rats |
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