Staphylococcus Aureus Infecton in Various Skin Diseases (1) Modulation of Antibody Level to Staphylokinase
An adequate serum marker for Staphylococcus aureus-infection has not been commonly utilized yet in clinical dermatology, when compared to ASO and ASK for streptococcusinfection. We measured antibody level of staphylokinase (SAK), a protein product of Staphylococcus aureus, in various skin diseases,...
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| Published in | Skin research Vol. 38; no. 5; pp. 491 - 496 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
Meeting of Osaka Dermatological Association
1996
日本皮膚科学会大阪地方会 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0018-1390 1884-541X |
| DOI | 10.11340/skinresearch1959.38.491 |
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| Abstract | An adequate serum marker for Staphylococcus aureus-infection has not been commonly utilized yet in clinical dermatology, when compared to ASO and ASK for streptococcusinfection. We measured antibody level of staphylokinase (SAK), a protein product of Staphylococcus aureus, in various skin diseases, in order to assess practical and investigative significance of and SAK. Levels of and SAK-IgG, IgA and IgM were measured with ELISA technique. In comparison to normal healthy subjects, patients with cellulitis and other infectious skin disease showed significantly high antibody level, and this level was not correlated to ASO or ASK. Patients with atopic dermatitis, nummular eczema, urticaria, erythema exsudativum mutiforme and erythema nodosum showed elevated anti SAK, suggesting a role of staphylococcal infection in those diseases. Western blotting confirmed specific immunoreaction bands to SAK in the blood samples from the patients with high anti-SAK titer. We conclude that antibody to SAK may be a worthy indicator to investigate the effects of Staphylococcus aureus-infection in various skin diseases. |
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| AbstractList | An adequate serum marker for Staphylococcus aureus-infection has not been commonly utilized yet in clinical dermatology, when compared to ASO and ASK for streptococcusinfection. We measured antibody level of staphylokinase (SAK), a protein product of Staphylococcus aureus, in various skin diseases, in order to assess practical and investigative significance of and SAK. Levels of and SAK-IgG, IgA and IgM were measured with ELISA technique. In comparison to normal healthy subjects, patients with cellulitis and other infectious skin disease showed significantly high antibody level, and this level was not correlated to ASO or ASK. Patients with atopic dermatitis, nummular eczema, urticaria, erythema exsudativum mutiforme and erythema nodosum showed elevated anti SAK, suggesting a role of staphylococcal infection in those diseases. Western blotting confirmed specific immunoreaction bands to SAK in the blood samples from the patients with high anti-SAK titer. We conclude that antibody to SAK may be a worthy indicator to investigate the effects of Staphylococcus aureus-infection in various skin diseases.
各種皮膚疾患患者血清中の抗スタフィロキナーゼ (SAK) 抗体価をEL, ISA法を用いて測定した。抗SAK-IgG抗体価を標準血清との比較による相対値で表すと, 皮膚感染症群では106.0±11.3であり正常群 (54.8±9.5) より有意に高値であった。この他, アトピー性皮膚炎, 貨幣状湿疹, 蕁麻疹, 多形滲出性紅斑, 結節性紅斑に於いても正常群に比べて有意な上昇を認めた。また同時に測定したASK, ASOの抗体価とは相関せず, 溶連菌感染からは独立した指標であることを示した。抗SAK抗体価の測定はブドウ球菌と皮膚疾患との関連を究明する上で有用な手段となりうることが示唆された。 An adequate serum marker for Staphylococcus aureus-infection has not been commonly utilized yet in clinical dermatology, when compared to ASO and ASK for streptococcusinfection. We measured antibody level of staphylokinase (SAK), a protein product of Staphylococcus aureus, in various skin diseases, in order to assess practical and investigative significance of and SAK. Levels of and SAK-IgG, IgA and IgM were measured with ELISA technique. In comparison to normal healthy subjects, patients with cellulitis and other infectious skin disease showed significantly high antibody level, and this level was not correlated to ASO or ASK. Patients with atopic dermatitis, nummular eczema, urticaria, erythema exsudativum mutiforme and erythema nodosum showed elevated anti SAK, suggesting a role of staphylococcal infection in those diseases. Western blotting confirmed specific immunoreaction bands to SAK in the blood samples from the patients with high anti-SAK titer. We conclude that antibody to SAK may be a worthy indicator to investigate the effects of Staphylococcus aureus-infection in various skin diseases. |
| Author | KABURAGI, Yutaka KITAMURA, Keijiro GOTO, Yoshiko IZAKI, Seiichi |
| Author_FL | 後藤 佳子 伊崎 誠一 北村 啓次郎 鏑 木豊 |
| Author_FL_xml | – sequence: 1 fullname: 鏑 木豊 – sequence: 2 fullname: 後藤 佳子 – sequence: 3 fullname: 伊崎 誠一 – sequence: 4 fullname: 北村 啓次郎 |
| Author_xml | – sequence: 1 fullname: KABURAGI, Yutaka organization: Department of Dermatology, Saitama Medical Center, Saitama Medical School – sequence: 1 fullname: KITAMURA, Keijiro organization: Department of Dermatology, Saitama Medical Center, Saitama Medical School – sequence: 1 fullname: GOTO, Yoshiko organization: Department of Dermatology, Saitama Medical Center, Saitama Medical School – sequence: 1 fullname: IZAKI, Seiichi organization: Department of Dermatology, Saitama Medical Center, Saitama Medical School |
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| DocumentTitleAlternate | 黄色ブドウ球菌感染と皮膚疾患との関連（第１報） 種々の皮膚疾患における黄色ブドウ球菌感染の新たなマーカー 抗スタフィロキナーゼ抗体の研究 (1) Modulation of Antibody Level to Staphylokinase 種々の皮膚疾患における黄色ブドウ球菌感染の新たなマーカー;抗スタフィロキナーゼ抗体の研究 |
| DocumentTitle_FL | 黄色ブドウ球菌感染と皮膚疾患との関連（第１報） 種々の皮膚疾患における黄色ブドウ球菌感染の新たなマーカー 抗スタフィロキナーゼ抗体の研究 |
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| References | 8. 宮野径彰: アトピー性皮膚炎と黄色ブドウ球菌, 皮膚科Mook17, 細菌性皮膚疾患, 荒田次郎編, 金原出版, 東京, 1990, 201-213 7. Izaki S, Goto Y, Kitamura K, Nomaguchi H: Antibody to 65-kD Stress Protein (HSP65) of Mycobacterium leprae in various inflammatory skin disease, A preliminary report, Jpn J Leprosy, 63: 3-11, 1994 4. Collen D, De Cock F, Stassen JM: Comparative immunogenicity and thrombolytic properties toward arterial and venous thrombi of streptokinase and recombinant staphylokinase in baboons, Circulation, 87: 996-1006, 1993 9. Leyden JJ, Morples RR, Kligman AM: Staphylococcus aureus in the lesion of atopic dermatitis, Br J Dermatol, 90: 525-530, 1974 5. 荒田次郎: 皮膚一般細菌感染症の動向, 皮膚科Mook17, 細菌性皮膚疾患, 荒田次郎編, 金原出版, 東京, 1990, 1-9 10. 野口俊彦, 向井秀樹, 西岡清, 西山茂夫: 成人型アトピー性皮膚炎の顔面酒様皮膚炎における細菌の関与, 日皮会誌, 100: 929-934, 1990 1. Sako T: Overproduction of staphylokinase in escherichia coli and its characterization, Eur J Biochem, 149: 557-563, 1985 14. 小野友道: 掌蹠膿疱症-特に扁摘の効果について, 皮膚臨床, 24: 1205-1213, 1982 6. Cohen D, Silence K, Demarsin E, DeMol M, Lijnen HR: Isolation and characterisation of natural and recombinant staphylokinase, Fibrinolysis, 6: 203-213, 1992 12. Gorden H: Erythema nodosum. Review of 115 cases, Br J Dermatol, 71: 393-409, 1961 2. Lack CH: Staphylokinase: an activator of plasma protease, Nature, 161: 559-560, 1948 3. Declerck PJ, Vanderschueren S, Billiet J, Moreau H, Collen D: Prevalence and induction of circulating antibodies against recombinant staphylokinase, Thromb Haemost, 71: 129-133, 1994 11. 朝田康夫: 貨幣状湿疹, 現代皮膚科学大系, 14, 山村雄一, 久木田淳, 佐野栄春, 清寺真編, 中山書店, 東京, 1980, 135-142 13. Allen D M, Diamond L K, Howell D A: Anaphylactoid purpura in children (Schonlein-Henoch Syndrome), Am J Dis Child, 99: 833-854, 1960 |
| References_xml | – reference: 4. Collen D, De Cock F, Stassen JM: Comparative immunogenicity and thrombolytic properties toward arterial and venous thrombi of streptokinase and recombinant staphylokinase in baboons, Circulation, 87: 996-1006, 1993 – reference: 1. Sako T: Overproduction of staphylokinase in escherichia coli and its characterization, Eur J Biochem, 149: 557-563, 1985 – reference: 5. 荒田次郎: 皮膚一般細菌感染症の動向, 皮膚科Mook17, 細菌性皮膚疾患, 荒田次郎編, 金原出版, 東京, 1990, 1-9 – reference: 14. 小野友道: 掌蹠膿疱症-特に扁摘の効果について, 皮膚臨床, 24: 1205-1213, 1982 – reference: 9. Leyden JJ, Morples RR, Kligman AM: Staphylococcus aureus in the lesion of atopic dermatitis, Br J Dermatol, 90: 525-530, 1974 – reference: 2. Lack CH: Staphylokinase: an activator of plasma protease, Nature, 161: 559-560, 1948 – reference: 10. 野口俊彦, 向井秀樹, 西岡清, 西山茂夫: 成人型アトピー性皮膚炎の顔面酒様皮膚炎における細菌の関与, 日皮会誌, 100: 929-934, 1990 – reference: 8. 宮野径彰: アトピー性皮膚炎と黄色ブドウ球菌, 皮膚科Mook17, 細菌性皮膚疾患, 荒田次郎編, 金原出版, 東京, 1990, 201-213 – reference: 6. Cohen D, Silence K, Demarsin E, DeMol M, Lijnen HR: Isolation and characterisation of natural and recombinant staphylokinase, Fibrinolysis, 6: 203-213, 1992 – reference: 13. Allen D M, Diamond L K, Howell D A: Anaphylactoid purpura in children (Schonlein-Henoch Syndrome), Am J Dis Child, 99: 833-854, 1960 – reference: 12. Gorden H: Erythema nodosum. Review of 115 cases, Br J Dermatol, 71: 393-409, 1961 – reference: 11. 朝田康夫: 貨幣状湿疹, 現代皮膚科学大系, 14, 山村雄一, 久木田淳, 佐野栄春, 清寺真編, 中山書店, 東京, 1980, 135-142 – reference: 3. Declerck PJ, Vanderschueren S, Billiet J, Moreau H, Collen D: Prevalence and induction of circulating antibodies against recombinant staphylokinase, Thromb Haemost, 71: 129-133, 1994 – reference: 7. Izaki S, Goto Y, Kitamura K, Nomaguchi H: Antibody to 65-kD Stress Protein (HSP65) of Mycobacterium leprae in various inflammatory skin disease, A preliminary report, Jpn J Leprosy, 63: 3-11, 1994 |
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| SubjectTerms | ELISA skin diseases Staphylococcus aureus Staphylokinase Western blot ウェスターンプロット法 スタフィロキナーゼ 皮膚疾患 黄色ブドウ球菌感染 |
| Subtitle | (1) Modulation of Antibody Level to Staphylokinase |
| Title | Staphylococcus Aureus Infecton in Various Skin Diseases |
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