Purity Test of Iodinated (125l) Insulin Solution, Iodinated (125l) IgE Solution and Iodinated (131l) PVP Injection Studies on the Quality and Test Method of Radiopharmaceuticals (4)

• Published in: RADIOISOTOPES Vol. 23; no. 6; pp. 342 - 346
• Main Authors: IKEBUCHI, Hideharu, URAKUBO, Goro, NAGAMATSU, Kunisuke
• Format: Journal Article
• Language: Japanese
• Published: Japan Radioisotope Association 15.06.1974
• ISSN: 0033-8303; 1884-4111
• DOI: 10.3769/radioisotopes.23.6_342

Gel filtration with thin layer made of Sephadex was applied to the purity test of 3 kinds of radiopharmaceuticals containing iodinated (125Ior131I) high molecular substances. Sephadex G-50, Superfine was used to make thin layer for iodinated (125I) insulin solution and iodinated (131I) polyvinylpyrrolidone injection, and Sephadex G-200, Superfine was used for iodinated (125I) IgE solution, according to the molecular weights of these radiopharmaceuticals. Drops of these radiopharmaceuticals were spotted on wet thin layer plates and developed by declining technique with water for 1.5-5 hours. The distribution of radioactivities on these chromatograms was measured, and a sharp peak corresponding to the pure iodinated (125Ior131I) substance was observed in each case. So, this gel filtration procedure was regarded as a suitable method for purity test of these radiopharmaceuticals, detecting radioactivities due to possible degradation product and free iodine.