Polymerase Chain Reaction による鶏敷料中の Campylobacter jejuni の特異的検出(短報)
Polymerase Chain Reaction 法を用いて, Campylobacter jejuni の検出を試みた. C. jejuni の flagellin A遺伝子のDNAシークエンスから設計したプライマーを用いてPCRを行った. このプライマーはC. jejuni を特異的に検出したが, C. coil, C. fetus, サルモネラ, 大腸菌DNAは増幅しなかった. また, この方法によって鶏敷料中に接種したC.jejuni についても, 特異的に検出することができた....
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| Published in | Journal of Veterinary Medical Science Vol. 57; no. 1; pp. 125 - 127 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
公益社団法人 日本獣医学会
15.02.1995
|
| Online Access | Get full text |
| ISSN | 0916-7250 1347-7439 |
| DOI | 10.1292/jvms.57.125 |
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| Abstract | Polymerase Chain Reaction 法を用いて, Campylobacter jejuni の検出を試みた. C. jejuni の flagellin A遺伝子のDNAシークエンスから設計したプライマーを用いてPCRを行った. このプライマーはC. jejuni を特異的に検出したが, C. coil, C. fetus, サルモネラ, 大腸菌DNAは増幅しなかった. また, この方法によって鶏敷料中に接種したC.jejuni についても, 特異的に検出することができた. |
|---|---|
| AbstractList | Polymerase Chain Reaction 法を用いて, Campylobacter jejuni の検出を試みた. C. jejuni の flagellin A遺伝子のDNAシークエンスから設計したプライマーを用いてPCRを行った. このプライマーはC. jejuni を特異的に検出したが, C. coil, C. fetus, サルモネラ, 大腸菌DNAは増幅しなかった. また, この方法によって鶏敷料中に接種したC.jejuni についても, 特異的に検出することができた. |
| Author | 斎藤, 志保子 八柳, 淳 伊藤, 隆 |
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| References | Picken, R. N., Wang, Z., and Yang, H. L. 1987. Mol. Cell. Probes 1: 245-259. Akiyama, M., Hayashi, M., Shiozawa, K., Nishina, T., and Murakami, M. 1987. J. Vet. Med. (Tokyo) 790: 279-299 (in Japanese). Taylor, D. E. and Hiratsuka, K. 1990. Mol. Cell. Probes 4: 261-271. Fischer, S. H. and Nachamkin, I. 1991. Mol. Microbiol. 5: 1151-1158. Grant, I. H., Richardson, N. J., and Bokkenheuser, V. D. 1980. J. Clin. Microbiol. 11: 508-510. Kazwala, R. R., Collins, J. D., Hannan, J., Crinion, R. A. P., and O'Mahony, H. 1990. Vet. Res. 126: 305-306. Oyofo, B. A., Thorton, S. A., Burr, D. H., Trust, T. J., and Guerry, P. 1992. J. Clin. Microbiol. 30: 2613-2619. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Ellich, H. A. 1988. Science 239: 487-491. Olive, D. M., Johny, M., and Sethi, S. K. 1990. J. Clin. Microbiol. 28: 1565-1569. Frankel, G., Giron, J. A., Valmassoi, J., and Schoolnik, G. K. 1989. Mol. Microbiol. 3: 1729-1734. Wempe, J. M., Genigeorgis, C. A., Farver, T. B., and Yusufu, H. A. 1983. Appl. Environ. Microbiol. 45: 355-359. Chuma, T., Yamada, T., Okamoto, K., Yugi, H., and Ohya, T. 1993. J. Vet. Med. Sci. 55: 1027-1029. Korolik, V., Coloe, P., and Krishnapillai, V. 1988. J. Gen. Microbiol. 134: 521-529. Genigeornis, C., Hssuneh, M., and Collins, P. 1986. J. Food Prot. 49: 895-903. Karmali, M. A., Penner, J. L., Fleming, P. C., Williams, A., and Hennessy, J. N. 1983. J. Infect. Dis. 147: 243-246. Eyers, M., Chapelle, S., Van Camp, G., Goossens, H., and De Washter, R. 1993. J. Clin. Microbiol. 31: 3340-3343. Nuijten, P. J. M., van Asten, F. J. A. M., Gaastra, W., and van der Zeijst, B. A. M. 1990. J. Biol. Chem. 265: 17798-17804. Jones, F. T., Axtell, R. C., Rives, D. V., Scheideler, S. E., Tarver, F. R. Jr., Walker, R. L., and Wineland, M. J. 1991. J. Food Prot. 54: 259-262. Itoh, T. and Yanagawa, Y. 1987. J. Vet. Med. (Tokyo) 790: 279-286 (in Japanese). |
| References_xml | – reference: Eyers, M., Chapelle, S., Van Camp, G., Goossens, H., and De Washter, R. 1993. J. Clin. Microbiol. 31: 3340-3343. – reference: Kazwala, R. R., Collins, J. D., Hannan, J., Crinion, R. A. P., and O'Mahony, H. 1990. Vet. Res. 126: 305-306. – reference: Grant, I. H., Richardson, N. J., and Bokkenheuser, V. D. 1980. J. Clin. Microbiol. 11: 508-510. – reference: Wempe, J. M., Genigeorgis, C. A., Farver, T. B., and Yusufu, H. A. 1983. Appl. Environ. Microbiol. 45: 355-359. – reference: Picken, R. N., Wang, Z., and Yang, H. L. 1987. Mol. Cell. Probes 1: 245-259. – reference: Akiyama, M., Hayashi, M., Shiozawa, K., Nishina, T., and Murakami, M. 1987. J. Vet. Med. (Tokyo) 790: 279-299 (in Japanese). – reference: Taylor, D. E. and Hiratsuka, K. 1990. Mol. Cell. Probes 4: 261-271. – reference: Karmali, M. A., Penner, J. L., Fleming, P. C., Williams, A., and Hennessy, J. N. 1983. J. Infect. Dis. 147: 243-246. – reference: Olive, D. M., Johny, M., and Sethi, S. K. 1990. J. Clin. Microbiol. 28: 1565-1569. – reference: Chuma, T., Yamada, T., Okamoto, K., Yugi, H., and Ohya, T. 1993. J. Vet. Med. Sci. 55: 1027-1029. – reference: Fischer, S. H. and Nachamkin, I. 1991. Mol. Microbiol. 5: 1151-1158. – reference: Nuijten, P. J. M., van Asten, F. J. A. M., Gaastra, W., and van der Zeijst, B. A. M. 1990. J. Biol. Chem. 265: 17798-17804. – reference: Frankel, G., Giron, J. A., Valmassoi, J., and Schoolnik, G. K. 1989. Mol. Microbiol. 3: 1729-1734. – reference: Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Ellich, H. A. 1988. Science 239: 487-491. – reference: Itoh, T. and Yanagawa, Y. 1987. J. Vet. Med. (Tokyo) 790: 279-286 (in Japanese). – reference: Jones, F. T., Axtell, R. C., Rives, D. V., Scheideler, S. E., Tarver, F. R. Jr., Walker, R. L., and Wineland, M. J. 1991. J. Food Prot. 54: 259-262. – reference: Korolik, V., Coloe, P., and Krishnapillai, V. 1988. J. Gen. Microbiol. 134: 521-529. – reference: Oyofo, B. A., Thorton, S. A., Burr, D. H., Trust, T. J., and Guerry, P. 1992. J. Clin. Microbiol. 30: 2613-2619. – reference: Genigeornis, C., Hssuneh, M., and Collins, P. 1986. J. Food Prot. 49: 895-903. |
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| Title | Polymerase Chain Reaction による鶏敷料中の Campylobacter jejuni の特異的検出(短報) |
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