C5 Contents and Neutrohil Chemotactic Activities in Bronchiolar and Alveolar Regions
To clarify the localization and mechanism of neutrophil infiltration in the lower respiratory tract, we measured neutrophil number, neutrophil chemotactic factor (NCF) activity and content of C5 in bronchial lavage (BL) fluid and bronchoalveolar lavage (BAL) fluid. Numbers of neutrophils, NCF activi...
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| Published in | The Japanese journal of thoracic diseases Vol. 29; no. 6; pp. 677 - 684 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
The Japanese Respiratory Society
25.06.1991
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0301-1542 1883-471X |
| DOI | 10.11389/jjrs1963.29.677 |
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| Abstract | To clarify the localization and mechanism of neutrophil infiltration in the lower respiratory tract, we measured neutrophil number, neutrophil chemotactic factor (NCF) activity and content of C5 in bronchial lavage (BL) fluid and bronchoalveolar lavage (BAL) fluid. Numbers of neutrophils, NCF activity and C5 content were higher in the BL fluid from normal volunteers (NV) and control patients (CP) than those in the BAL fluid from the same subjects. The NCF activity in the BL fluid was inhibited approximately 40% by anti-C5 antiserum, and correlated with C5 content in the BL fluid. In the BAL fluids of patients with chronic airway diseases (CAD) and patients with idiopathic interstitial pneumonia (IIP), neurtophil number, NCF activity and C5 content were increased compared to those in BAL fluid from NV or CP. These results indicated that neutrophils are predominant in the bronchial region compared to the alveolar region, and that C5-derived NCF play important roles in the accumulation of neutrophils in the bronchial region. Also C5-derived NCF are thought to be related to, at least, a part of the neutrophil infiltration in the respiratory tract of patients with CAD and IIP. |
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| AbstractList | To clarify the localization and mechanism of neutrophil infiltration in the lower respiratory tract, we measured neutrophil number, neutrophil chemotactic factor (NCF) activity and content of C5 in bronchial lavage (BL) fluid and bronchoalveolar lavage (BAL) fluid. Numbers of neutrophils, NCF activity and C5 content were higher in the BL fluid from normal volunteers (NV) and control patients (CP) than those in the BAL fluid from the same subjects. The NCF activity in the BL fluid was inhibited approximately 40% by anti-C5 antiserum, and correlated with C5 content in the BL fluid. In the BAL fluids of patients with chronic airway diseases (CAD) and patients with idiopathic interstitial pneumonia (IIP), neurtophil number, NCF activity and C5 content were increased compared to those in BAL fluid from NV or CP. These results indicated that neutrophils are predominant in the bronchial region compared to the alveolar region, and that C5-derived NCF play important roles in the accumulation of neutrophils in the bronchial region. Also C5-derived NCF are thought to be related to, at least, a part of the neutrophil infiltration in the respiratory tract of patients with CAD and IIP. |
| Author | Yasuoka, Susumu Kamei, Toshihiko Hayashi, Hideki Tani, Kenji Ozaki, Toshio Noda, Yasuhiro Ogushi, Fumitaka Nakamura, Youichi Kawaji, Koji Ogura, Takeshi |
| Author_xml | – sequence: 1 fullname: Ozaki, Toshio organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Ogura, Takeshi organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Hayashi, Hideki organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Nakamura, Youichi organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Kawaji, Koji organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Ogushi, Fumitaka organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Noda, Yasuhiro organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Yasuoka, Susumu organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Kamei, Toshihiko organization: The Third Department of Internal Medicine, Tokushima University – sequence: 1 fullname: Tani, Kenji organization: The Third Department of Internal Medicine, Tokushima University |
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| References | 18) Matzner, Y. & Brzezinski, A.: A C5 inhibitor in peritoneal fluid. J. Clin. Med., 103: 227, 1984. 16) Scherzer, H. H., Kreuzer, D. L., Varani, J., Desai, U. & Senior, R. M.: Demonstration of the synthesis of the third and fifth components of complement by human alveolar macrophages. Am. Rev. Respir. Dis., 121: 92A, 1980. 1) Weinberger, S. E., Kelmen, J. A., Elson, N. A., Young, R. C., Reynolds, H. Y., Fulmer, J. D. & Crystal, R. G.: Bronchoalveolar lavage in interstitial lung disease. Ann. Intern. Med., 89: 459, 1978. 6) Janoff, A.: Elastase and emphysema: Current assessment of the protease-antiprotease hypothesis. Am. Rev. Respir. Dis., 132: 417, 1985. 12) Ward, P. A. & Newman, L. J.: A neutrophil chemotactic factor from human C5. J. Immunol., 102: 93, 1969. 14) Hunninghake, G. W., Gadek, J. E., Lawley, T. J. & Crystal, R. G.: Mechanisms of neutrohil accumulation in the lung of patients with idioathic pulmonary fibrosis. J. Clin. Invest., 68: 259, 1981. 3) Yasuoka, S., Nakayama, T., Kawano, T., Ogushi, F., Doi, H., Hayashi, H. & Tsubura, E.: Comparisone of cell profiles of bronchial and bronchoalveolar lavage fluids between normal subjects and patients with idiopathic pulmonary fibrosis. Tohoku J. Exp. Med., 146: 33, 1985. 8) 田村昌士: 特発性間質性肺炎の診断基準. 厚生省特定疾患, 間質性肺疾患調査研究班, 昭和55年研究報告書, 1980, p. 4. 4) 尾崎敏夫, 中山正, 林秀樹, 河野知弘, 大串文隆, 安岡劭, 螺良英郎: 気管支肺胞洗浄液と気管支洗浄液の細胞成分の比較. 気管支学, 6: 558, 1984. 10) Böyum, A.: Isolation of mononuclear cells and granulocytes from human blood: Isolation of mononuclear cells by one centrifugation and of granulocytes by combining centrifugation and sedimentation at 1g. Scand. J. Lab. Invest., 27: 77, 1968. 5) Pennington, J. E., Reynolds, H. Y. & Carbone, P. P.: Pseudomonas pneumonia. A retrospective study of 36 cases. Am. J. Med., 55: 153, 1973. 17) Robbins, R. A., Justice, J. M., Rasmussen, J. K., Russ, W. D., Thomas, K. R. & Rennard, S. I.: The role of chemotactic factor inactivator in modulating alveolar macrophage-derived neutrophil chemotactic activity. J. Lab. Clin. Med., 109: 164, 1987. 7) Weiland, J. E., Davis, W. B., Holter, J. F., Mohammed, J. R., Dovinsky, P. M. & Gadek, J. E.: Lung neutrophils in the adult respiratory distress syndrom. Clinical and pathophysiologic significance. Am. Rev. Respir. Dis., 133: 218, 1986. 2) 中山正, 安岡劭, 河野知弘, 島田久夫, 尾崎敏夫, 後東俊博, 久野梧郎, 螺良英郎: 気管支―肺胞洗浄液中に含まれる細胞成分と液性成分の分析. 日本胸部疾患学会雑誌, 18: 11, 1980. 11) Reynold, H. Y.: Lung infllammation: Role of endogenous chemotactic factors in attracting polymorphonuclear granulocytes. Am. Rev. Respir. Dis., 127: S16, 1983. 19) Robbins, R. A., Russ, W. D., Thomas, K. R., Rasmussen, J. K. & Kay, H. D.: Complement component C5 is required for release of alveolar macrophage-derived neutrophil chemotactic activity. Am. Rev. Respir. Dis., 135: 619, 1987. 13) Hopkins, H., Stull, T., Von Essen, S. G., Bobbins, R. A. & Rennard, S. I.: Neurophil chemotactic factors in bacterial pneumonia. Chest, 95: 1021, 1989. 9) Falk, W., Goodwin, R. H. & Leonard, E. J.: A48-well microchemotaxis assembly for rapid and accurate measurement of leukocyte migration. J. Immunol., 33: 239, 1980. 15) Robbins, R. A., Gadek, J. E., Rennard, S. I., Chen, Y. F. & Crystal, R. G.: Compartmentalization of complement system: The normal lung is C5 “deficient”. Am. Rev. Respir. Dis., 125: 55A, 1982. |
| References_xml | – reference: 19) Robbins, R. A., Russ, W. D., Thomas, K. R., Rasmussen, J. K. & Kay, H. D.: Complement component C5 is required for release of alveolar macrophage-derived neutrophil chemotactic activity. Am. Rev. Respir. Dis., 135: 619, 1987. – reference: 14) Hunninghake, G. W., Gadek, J. E., Lawley, T. J. & Crystal, R. G.: Mechanisms of neutrohil accumulation in the lung of patients with idioathic pulmonary fibrosis. J. Clin. Invest., 68: 259, 1981. – reference: 15) Robbins, R. A., Gadek, J. E., Rennard, S. I., Chen, Y. F. & Crystal, R. G.: Compartmentalization of complement system: The normal lung is C5 “deficient”. Am. Rev. Respir. Dis., 125: 55A, 1982. – reference: 17) Robbins, R. A., Justice, J. M., Rasmussen, J. K., Russ, W. D., Thomas, K. R. & Rennard, S. I.: The role of chemotactic factor inactivator in modulating alveolar macrophage-derived neutrophil chemotactic activity. J. Lab. Clin. Med., 109: 164, 1987. – reference: 5) Pennington, J. E., Reynolds, H. Y. & Carbone, P. P.: Pseudomonas pneumonia. A retrospective study of 36 cases. Am. J. Med., 55: 153, 1973. – reference: 4) 尾崎敏夫, 中山正, 林秀樹, 河野知弘, 大串文隆, 安岡劭, 螺良英郎: 気管支肺胞洗浄液と気管支洗浄液の細胞成分の比較. 気管支学, 6: 558, 1984. – reference: 1) Weinberger, S. E., Kelmen, J. A., Elson, N. A., Young, R. C., Reynolds, H. Y., Fulmer, J. D. & Crystal, R. G.: Bronchoalveolar lavage in interstitial lung disease. Ann. Intern. Med., 89: 459, 1978. – reference: 3) Yasuoka, S., Nakayama, T., Kawano, T., Ogushi, F., Doi, H., Hayashi, H. & Tsubura, E.: Comparisone of cell profiles of bronchial and bronchoalveolar lavage fluids between normal subjects and patients with idiopathic pulmonary fibrosis. Tohoku J. Exp. Med., 146: 33, 1985. – reference: 13) Hopkins, H., Stull, T., Von Essen, S. G., Bobbins, R. A. & Rennard, S. I.: Neurophil chemotactic factors in bacterial pneumonia. Chest, 95: 1021, 1989. – reference: 6) Janoff, A.: Elastase and emphysema: Current assessment of the protease-antiprotease hypothesis. Am. Rev. Respir. Dis., 132: 417, 1985. – reference: 18) Matzner, Y. & Brzezinski, A.: A C5 inhibitor in peritoneal fluid. J. Clin. Med., 103: 227, 1984. – reference: 16) Scherzer, H. H., Kreuzer, D. L., Varani, J., Desai, U. & Senior, R. M.: Demonstration of the synthesis of the third and fifth components of complement by human alveolar macrophages. Am. Rev. Respir. Dis., 121: 92A, 1980. – reference: 9) Falk, W., Goodwin, R. H. & Leonard, E. J.: A48-well microchemotaxis assembly for rapid and accurate measurement of leukocyte migration. J. Immunol., 33: 239, 1980. – reference: 11) Reynold, H. Y.: Lung infllammation: Role of endogenous chemotactic factors in attracting polymorphonuclear granulocytes. Am. Rev. Respir. Dis., 127: S16, 1983. – reference: 10) Böyum, A.: Isolation of mononuclear cells and granulocytes from human blood: Isolation of mononuclear cells by one centrifugation and of granulocytes by combining centrifugation and sedimentation at 1g. Scand. J. Lab. Invest., 27: 77, 1968. – reference: 8) 田村昌士: 特発性間質性肺炎の診断基準. 厚生省特定疾患, 間質性肺疾患調査研究班, 昭和55年研究報告書, 1980, p. 4. – reference: 12) Ward, P. A. & Newman, L. J.: A neutrophil chemotactic factor from human C5. J. Immunol., 102: 93, 1969. – reference: 7) Weiland, J. E., Davis, W. B., Holter, J. F., Mohammed, J. R., Dovinsky, P. M. & Gadek, J. E.: Lung neutrophils in the adult respiratory distress syndrom. Clinical and pathophysiologic significance. Am. Rev. Respir. Dis., 133: 218, 1986. – reference: 2) 中山正, 安岡劭, 河野知弘, 島田久夫, 尾崎敏夫, 後東俊博, 久野梧郎, 螺良英郎: 気管支―肺胞洗浄液中に含まれる細胞成分と液性成分の分析. 日本胸部疾患学会雑誌, 18: 11, 1980. |
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| SubjectTerms | Chronic airway diseases Complement 5 Idiopathic interstitial pneumonia Neutrophil chemotactic factor |
| Title | C5 Contents and Neutrohil Chemotactic Activities in Bronchiolar and Alveolar Regions |
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