Middle Molecule Substances in the Hemofiltrate of Dialysis Patients I. Separation of Peptidic Substances by “Hydrophobic” HPLC
By applying a“hydrophobic”HPLC system, five of peptidic substances in the middle molecule range were separated from the hemofiltrate fluid of a dialysis patient with chronic uremia. This system was based on the hydrophobicity value of peptide hormones having molecular weight up to 5,000 daltons by u...
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| Published in | Japanese Journal of Clinical Chemistry Vol. 14; no. 5; pp. 327 - 336 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan Society of Clinical Chemistry
25.12.1985
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| Online Access | Get full text |
| ISSN | 0370-5633 2187-4077 |
| DOI | 10.14921/jscc1971b.14.5_327 |
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| Abstract | By applying a“hydrophobic”HPLC system, five of peptidic substances in the middle molecule range were separated from the hemofiltrate fluid of a dialysis patient with chronic uremia. This system was based on the hydrophobicity value of peptide hormones having molecular weight up to 5,000 daltons by using gradient elution with increasing acetonitrile concentration in the eluent. Many ultraviolet-absorbing solutes of the hemofiltrate detected on this system were classified as hydrophilics, low-hydrophobics and hydrophobics. Numerous peaks of the hydrophobic solutes could be detected only in the hemofiltrate but not in healthy subject urines. Both middle molecule crude fractions of low-hydrophobicity and hydrophobicity were separated by step-wise gradient elution and further separation of the middle molecule subfractions was achieved by isocratic elution. Each of five subfractions corresponded to a single middle molecule and was found to be peptidic substances. |
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| AbstractList | By applying a“hydrophobic”HPLC system, five of peptidic substances in the middle molecule range were separated from the hemofiltrate fluid of a dialysis patient with chronic uremia. This system was based on the hydrophobicity value of peptide hormones having molecular weight up to 5,000 daltons by using gradient elution with increasing acetonitrile concentration in the eluent. Many ultraviolet-absorbing solutes of the hemofiltrate detected on this system were classified as hydrophilics, low-hydrophobics and hydrophobics. Numerous peaks of the hydrophobic solutes could be detected only in the hemofiltrate but not in healthy subject urines. Both middle molecule crude fractions of low-hydrophobicity and hydrophobicity were separated by step-wise gradient elution and further separation of the middle molecule subfractions was achieved by isocratic elution. Each of five subfractions corresponded to a single middle molecule and was found to be peptidic substances. |
| Author | TAKANO, KYOKO SAWANISHI, KENJI HORI, RYOHEI KONOBU, KANICHI TANAKA, KIMIKAZU INUI, KENICHI ISHIDA, MAYUMI |
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| References | 1) P. Contreras, R. Later, J. Navatto, J. L. Touraine, A. M. Freyria and J. Traeger: Nephron, 32, 193 (1982 3) T. Abiko, M. Kumikawa, H. Higuchi and H. Sekino: Biochem. Biophys. Res. Commun., 84, 184 (1978 8) J. L. Meek and Z. L. Rossetti: J. Chromatogr., 211, 15 (1981 6) I. Molnar and C. Horwath: J. Chromatogr., 142, 623 (1977 4) T. Abiko, M. Kumikawa, M. Ishizaki, H. Takahashi and H. Sekino: Biochem. Biopys. Res. Commun., 83, 357 (1978 9) F. C. Scenftleber, A. G. Hallina, H. Veening and D. A. Dayton: Clin. Chem., 51, 1522 (1976 2) N. K. Man, G. Cueille, J. Zingraff, J. Boudet, A. Sausse and J. L. Funk-Brentano: Artif. Organs, 4, 116 (1981 7) M. J. O'Hare and E. C. Nice: J. Chromatogr., 171, 209 (1979 10) D. W. Hill, F. H. Walters, T. D. Wilson and J. D. Stuart: Anal. Chem., 51, 1338 (1979 5) T. Abiko, I. Onodera and H. Sekino: Biochem. Biophys. Res. Commun., 89, 813 (1979 11) R. F. Rekker: The Hydrophobic Fragmental Constant. Elsevier, Amsterdam, New York p.301 (1977 |
| References_xml | – reference: 1) P. Contreras, R. Later, J. Navatto, J. L. Touraine, A. M. Freyria and J. Traeger: Nephron, 32, 193 (1982) – reference: 10) D. W. Hill, F. H. Walters, T. D. Wilson and J. D. Stuart: Anal. Chem., 51, 1338 (1979) – reference: 4) T. Abiko, M. Kumikawa, M. Ishizaki, H. Takahashi and H. Sekino: Biochem. Biopys. Res. Commun., 83, 357 (1978) – reference: 6) I. Molnar and C. Horwath: J. Chromatogr., 142, 623 (1977) – reference: 7) M. J. O'Hare and E. C. Nice: J. Chromatogr., 171, 209 (1979) – reference: 5) T. Abiko, I. Onodera and H. Sekino: Biochem. Biophys. Res. Commun., 89, 813 (1979) – reference: 2) N. K. Man, G. Cueille, J. Zingraff, J. Boudet, A. Sausse and J. L. Funk-Brentano: Artif. Organs, 4, 116 (1981) – reference: 11) R. F. Rekker: The Hydrophobic Fragmental Constant. Elsevier, Amsterdam, New York p.301 (1977) – reference: 8) J. L. Meek and Z. L. Rossetti: J. Chromatogr., 211, 15 (1981) – reference: 3) T. Abiko, M. Kumikawa, H. Higuchi and H. Sekino: Biochem. Biophys. Res. Commun., 84, 184 (1978) – reference: 9) F. C. Scenftleber, A. G. Hallina, H. Veening and D. A. Dayton: Clin. Chem., 51, 1522 (1976) |
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| Subtitle | I. Separation of Peptidic Substances by “Hydrophobic” HPLC |
| Title | Middle Molecule Substances in the Hemofiltrate of Dialysis Patients |
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