Current Advances and Future Prospects for Apical-anaerobic Cultivation Devices

Intestinal epithelial cells face an aerobic environment on their basal side, while their apical side is exposed to an anaerobic environment, where a diverse range of obligate anaerobic microbes dwell and establish a symbiotic relationship with the host. To reconstruct the complex intestinal ecosyste...

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Published inChōnai saikingaku zasshi Vol. 38; no. 4; pp. 219 - 228
Main Author KATAYAMA, Takane
Format Journal Article
LanguageEnglish
Japanese
Published Tokyo The Intestinal Microbiology Society 2024
公益財団法人 腸内細菌学会
Japan Science and Technology Agency
Subjects
Anaerobic processes
apical-anaerobic cultivation device
co-cultivation
Cultivation
Epithelial cells
Epithelium
gut microbe
intestinal epithelial cell
Intestine
Microorganisms
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ISSN1343-0882
1349-8363
DOI10.11209/jim.38.219

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Summary:Intestinal epithelial cells face an aerobic environment on their basal side, while their apical side is exposed to an anaerobic environment, where a diverse range of obligate anaerobic microbes dwell and establish a symbiotic relationship with the host. To reconstruct the complex intestinal ecosystem and understand the molecular basis underlying the crosstalk between gut microbes and the host, it is necessary to develop a device that can reproduce these two different oxygen concentration environments experienced by intestinal epithelial cells. I have collaborated with two companies to jointly develop such a device. In this review article, I briefly introduce the development process of this apical anaerobic cultivation device, and highlight some of the results obtained using the prototype and manufactured devices to discuss their effectiveness as research tools.
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ISSN:1343-0882
1349-8363
DOI:10.11209/jim.38.219