Exploring Boundary Effects on Oxygen and Cell Distribution in 3D-Printed Helical Hydrogel: Computational Insights
Developing a functional vascular network within thick hydrogel scaffolds is crucial for efficiently exchanging oxygen and nutrients, removing waste materials, and overall performance of engineered tissues. Recently, 3D bio-printing with coaxial nozzles and sacrificial ink has gained significant atte...
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Published in | 2023 30th National and 8th International Iranian Conference on Biomedical Engineering (ICBME) pp. 226 - 231 |
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Main Authors | , , , |
Format | Conference Proceeding |
Language | English |
Published |
IEEE
30.11.2023
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Subjects | |
Online Access | Get full text |
DOI | 10.1109/ICBME61513.2023.10488646 |
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Abstract | Developing a functional vascular network within thick hydrogel scaffolds is crucial for efficiently exchanging oxygen and nutrients, removing waste materials, and overall performance of engineered tissues. Recently, 3D bio-printing with coaxial nozzles and sacrificial ink has gained significant attention for creating complex structures with hollow perfusable channels. Current technology cannot evaluate mass transport during vascularization and perfusion of the culture medium into the channels of a porous hydrogel scaffold. Here, a simulation was employed to investigate the effects of boundary conditions on oxygen delivery, cellular growth, and viability within a 3D-printed helical hydrogel using the COMSOL Multiphysics software. Navier-Stokes and Brinkman equations were used to simulate fluid flow in the channel and porous medium, respectively. Concentration boundary condition (uniform oxygen concentration at channel walls) significantly increased the cell density from 2\times 10^{10}\mathrm{~m}^{-3} to over 9\times 10^{11}\mathrm{~m}^{-3} within one month. The oxygen concentration at the outlet of the concentration boundary condition remained consistently high at 180\mu\mathrm{M}. However, in the wall boundary condition (no exchange of oxygen at channel walls), the oxygen concentration at the end of the channel fell below the critical threshold for cell survival (14 \mu\mathrm{M}). Additionally, the average cell density observed was 1\times 10^{11}\mathrm{~m}^{-3}. These findings underline the substantial influence of boundary conditions on cell proliferation within the 3D scaffold. |
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AbstractList | Developing a functional vascular network within thick hydrogel scaffolds is crucial for efficiently exchanging oxygen and nutrients, removing waste materials, and overall performance of engineered tissues. Recently, 3D bio-printing with coaxial nozzles and sacrificial ink has gained significant attention for creating complex structures with hollow perfusable channels. Current technology cannot evaluate mass transport during vascularization and perfusion of the culture medium into the channels of a porous hydrogel scaffold. Here, a simulation was employed to investigate the effects of boundary conditions on oxygen delivery, cellular growth, and viability within a 3D-printed helical hydrogel using the COMSOL Multiphysics software. Navier-Stokes and Brinkman equations were used to simulate fluid flow in the channel and porous medium, respectively. Concentration boundary condition (uniform oxygen concentration at channel walls) significantly increased the cell density from 2\times 10^{10}\mathrm{~m}^{-3} to over 9\times 10^{11}\mathrm{~m}^{-3} within one month. The oxygen concentration at the outlet of the concentration boundary condition remained consistently high at 180\mu\mathrm{M}. However, in the wall boundary condition (no exchange of oxygen at channel walls), the oxygen concentration at the end of the channel fell below the critical threshold for cell survival (14 \mu\mathrm{M}). Additionally, the average cell density observed was 1\times 10^{11}\mathrm{~m}^{-3}. These findings underline the substantial influence of boundary conditions on cell proliferation within the 3D scaffold. |
Author | Zaman, MohammadSadegh Tarkhaneh, MohammadAmin Arshadi, Ahmad Saadatmand, Maryam |
Author_xml | – sequence: 1 givenname: MohammadSadegh surname: Zaman fullname: Zaman, MohammadSadegh email: msadegh.zaman@sharif.edu organization: Sharif University of Technology,Department of Chemical and Petroleum Engineering,Tehran,Iran – sequence: 2 givenname: Maryam surname: Saadatmand fullname: Saadatmand, Maryam email: m.saadatmand@sharif.edu organization: Sharif University of Technology,Department of Chemical and Petroleum Engineering,Tehran,Iran – sequence: 3 givenname: Ahmad surname: Arshadi fullname: Arshadi, Ahmad email: ahmadd.arshadii@gmail.com organization: Sharif University of Technology,Department of Chemical and Petroleum Engineering,Tehran,Iran – sequence: 4 givenname: MohammadAmin surname: Tarkhaneh fullname: Tarkhaneh, MohammadAmin email: moh.amin.t78@gmail.com organization: Sharif University of Technology,Department of Chemical and Petroleum Engineering,Tehran,Iran |
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SubjectTerms | 3D simulation Boundary conditions Cell density Coaxial nozzles Helical hydrogel Hydrogels Ink Oxygen Oxygen delivery Spirals Three-dimensional displays Transmission line matrix methods Waste materials |
Title | Exploring Boundary Effects on Oxygen and Cell Distribution in 3D-Printed Helical Hydrogel: Computational Insights |
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