Retention of the p53 Codon 72 Arginine Allele Is Associated with a Reduction of Disease-Free and Overall Survival in Arginine/Proline Heterozygous Breast Cancer Patients

• Published in: Clinical cancer research Vol. 9; no. 13; pp. 4860 - 4864
• Main Authors: BONAFE, Massimiliano, CECCARELLI, Claudio, FRANCESCHI, Claudio, FARABEGOLI, Fulvia, SANTINI, Donatella, TAFFURELLI, Mario, BARBI, Cristiana, MARZI, Erika, TRAPASSI, Chiara, STORCI, Gianluca, OLIVIERI, Fabiola
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.10.2003
• Subjects: Adult; Aged; Aged, 80 and over; Alleles; Arginine - genetics; Biological and medical sciences; Breast Neoplasms - genetics; Breast Neoplasms - mortality; Cell Line, Tumor; Codon; Disease-Free Survival; DNA - genetics; DNA - metabolism; Female; Genes, p53; Genotype; Gynecology. Andrology. Obstetrics; Heterozygote; Humans; Immunohistochemistry; Lymphatic Metastasis; Mammary gland diseases; Medical sciences; Middle Aged; Nucleic Acid Hybridization; Polymorphism, Genetic; Prognosis; Proline; Time Factors; Tumors; Human; Prognosis; Genotype; Malignant tumor; Survival; Mammary gland diseases; Allele; Codon; TP53 Gene; Arginine; Molecular epidemiology; Genetics; Female; Mammary gland; Predictive factor; Tumor suppressor gene; Polymorphism
• ISSN: 1078-0432; 1557-3265

Purpose: The arginine to proline substitution at codon 72 represents a common aminoacidic polymorphism of the p53 protein. Recent data suggest that p53 codon 72 may modulate the response to cancer therapy. The aim of this study was to test the hypothesis that the p53 codon 72 genotype, evaluated in the tumor tissue and in the disease-free lymph node, is related to differences in disease-free and overall survival among breast cancer-affected patients. Experimental Design: We assessed the p53 codon 72 genotype in DNA from disease-free lymph nodes and neoplastic tissues obtained from 67 women affected by breast cancer who underwent surgical resection at the Bologna Breast Cancer Surgical Unit from 1993 to 1995. Results: We found that the retention of the p53 codon 72 arginine allele in the tumor tissue of proline/arginine heterozygous breast cancer patients is associated with statistically significant reduced disease-free and overall survivals. Conclusions: Our findings suggest that the genotyping for p53 codon 72 locus in both the tumor tissue and in the lymph node of breast cancer patients could contribute to identify a subset of arginine/proline heterozygous patients who have a reduced survival that is associated with the specific retention of the arginine allele in the tumor tissue.