Detection of Fusarium Species by Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibody

Enzyme-linked immunosorbent assay was developed for the rapid detection of Fusarium species, known as harmful in food. One of the hybridoma cell lines which produced a monoclonal antibody specific to Fusarium extracellular polysaccharide was screened and the Mab was produced and purified. a detectio...

Full description

Saved in:
Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 13; no. 5; pp. 794 - 799
Main Authors Kwak, B.Y, Shon, D.H. (Korea Food Research Institute, Seongnam, Republic of Korea);Kwon, B.J., Kweon, C.H. (National Veterinary Research and Quarantine Service, Ahnyang, Republic of Korea)
Format Journal Article
LanguageEnglish
Published Seoul Korean Society for Applied Microbiology 01.10.2003
한국미생물·생명공학회
Subjects
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
fusarium
monoclonal antibodies
생물학
sandwich ELISA
Monoclonal antibody
Extracellular
Fungi
Analysis method
extracellular polysaccharide
enzyme-linked immunosorbent assay
Fusarium
Fungi Imperfecti
Detection
Polysaccharide
ELISA assay
Thallophyta
Online AccessGet full text
ISSN1017-7825

Cover

More Information
Summary:Enzyme-linked immunosorbent assay was developed for the rapid detection of Fusarium species, known as harmful in food. One of the hybridoma cell lines which produced a monoclonal antibody specific to Fusarium extracellular polysaccharide was screened and the Mab was produced and purified. a detection limit of the sandwich ELISA against F. moniliforme EPS was 0.001mg/ml in the standard curve.
Bibliography:L10
2004005391
G704-000169.2003.13.5.018
ISSN:1017-7825