Enzymology of the Anaphase‐Promoting Complex

The anaphase‐promoting complex (APC) is an ubiquitin‐protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both...

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Published inMethods in Enzymology Vol. 398; pp. 219 - 230
Main Authors Carroll, Christopher W., Morgan, David O.
Format Book Chapter Journal Article
LanguageEnglish
Published United States Elsevier Science & Technology 2005
Subjects
Online AccessGet full text
ISBN9780121828035
0121828034
ISSN0076-6879
1557-7988
DOI10.1016/S0076-6879(05)98018-X

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Abstract The anaphase‐promoting complex (APC) is an ubiquitin‐protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC.
AbstractList The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC.
Author Carroll, Christopher W.
Morgan, David O.
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Snippet The anaphase‐promoting complex (APC) is an ubiquitin‐protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins,...
The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins,...
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StartPage 219
SubjectTerms Anaphase-Promoting Complex-Cyclosome
Animals
Baculoviridae
Cadherins - biosynthesis
Cadherins - genetics
Cadherins - isolation & purification
Cell Line
Cloning, Molecular - methods
Cyclin B - isolation & purification
Cyclin B - metabolism
Genetic Vectors
Iodine Radioisotopes
Peptides - isolation & purification
Saccharomyces cerevisiae - enzymology
Sea Urchins
Sodium Iodide
Spodoptera
Ubiquitin - metabolism
Ubiquitin-Protein Ligase Complexes - isolation & purification
Ubiquitin-Protein Ligase Complexes - physiology
Title Enzymology of the Anaphase‐Promoting Complex
URI https://dx.doi.org/10.1016/S0076-6879(05)98018-X
https://www.ncbi.nlm.nih.gov/pubmed/16275331
Volume 398
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