Enzymology of the Anaphase‐Promoting Complex
The anaphase‐promoting complex (APC) is an ubiquitin‐protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both...
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Published in | Methods in Enzymology Vol. 398; pp. 219 - 230 |
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Main Authors | , |
Format | Book Chapter Journal Article |
Language | English |
Published |
United States
Elsevier Science & Technology
2005
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Subjects | |
Online Access | Get full text |
ISBN | 9780121828035 0121828034 |
ISSN | 0076-6879 1557-7988 |
DOI | 10.1016/S0076-6879(05)98018-X |
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Abstract | The anaphase‐promoting complex (APC) is an ubiquitin‐protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC. |
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AbstractList | The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC. |
Author | Carroll, Christopher W. Morgan, David O. |
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Snippet | The anaphase‐promoting complex (APC) is an ubiquitin‐protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins,... The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins,... |
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SubjectTerms | Anaphase-Promoting Complex-Cyclosome Animals Baculoviridae Cadherins - biosynthesis Cadherins - genetics Cadherins - isolation & purification Cell Line Cloning, Molecular - methods Cyclin B - isolation & purification Cyclin B - metabolism Genetic Vectors Iodine Radioisotopes Peptides - isolation & purification Saccharomyces cerevisiae - enzymology Sea Urchins Sodium Iodide Spodoptera Ubiquitin - metabolism Ubiquitin-Protein Ligase Complexes - isolation & purification Ubiquitin-Protein Ligase Complexes - physiology |
Title | Enzymology of the Anaphase‐Promoting Complex |
URI | https://dx.doi.org/10.1016/S0076-6879(05)98018-X https://www.ncbi.nlm.nih.gov/pubmed/16275331 |
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