原发性膜性肾病患者外周血lncRNA GAS5-AS1、白细胞介素-27表达及与Treg/Th17平衡的关系
目的 检测原发性膜性肾病(primary membranous nephropathy,PMN)患者外周血长链非编码RNA-生长停滞特异性转录本 5反义RNA1(long non-coding RNA growth arrest-specific transcript 5 antisense RNA1,lncRNA GAS5-AS1)、白细胞介素(interleukin,IL)-27表达,并分析其与患者调节性T细胞/辅助性T细胞 17(regulatory T cell/T help 17cells,Treg/Th17)平衡的关系.方法 选择 2019年 9月至 2020年 9月间河北以岭医院...
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| Published in | Linchuang Shenzangbing Zazhi Vol. 23; no. 12; pp. 995 - 1001 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
河北以岭医院肾病科,石家庄 050000
2023
Editorial Department of Journal of Clinical Nephrology |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-2390 |
| DOI | 10.3969/j.issn.1671-2390.2023.12.004 |
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| Abstract | 目的 检测原发性膜性肾病(primary membranous nephropathy,PMN)患者外周血长链非编码RNA-生长停滞特异性转录本 5反义RNA1(long non-coding RNA growth arrest-specific transcript 5 antisense RNA1,lncRNA GAS5-AS1)、白细胞介素(interleukin,IL)-27表达,并分析其与患者调节性T细胞/辅助性T细胞 17(regulatory T cell/T help 17cells,Treg/Th17)平衡的关系.方法 选择 2019年 9月至 2020年 9月间河北以岭医院收治的PMN患者 94例作为PMN组.另选取同期体检健康者 96例作为对照组.收集所有研究对象外周血标本,实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)法测定血清lncRNA GAS5-AS1水平、酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)法测定IL-27、IL-17、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-10、转化生长因子β1(transforming growth factor betal,TGF-β1)水平,流式细胞仪测定外周血Treg细胞、Th17细胞比例,并计算Treg/Th17比值.Pearson法分析PMN患者lncRNA GAS5-AS1、IL-27水平与IL-17、TNF-α、IL-10、TGF-β1、Treg/Th17的相关性.结果 对照组血尿素氮(blood urea nitrogen,BUN)为(4.03±0.66)mmol/L,PMN组患者BUN为(6.52±1.05)mmol/L;对照组血尿酸(uric acid,UA)为(266.13±44.35)μmol/L,PMN组患者UA为(338.92±56.45)μmol/L;对照组胱抑素C(cystatin C,Cys C)为(0.72±0.12)mg/L,PMN组患者Cys C为(1.38±0.23)mg/L;对照组抗磷脂酶 A2受体抗体(phospholipase A2 receptor antibody,PLA2R-Ab)为(9.37±1.92)RU/mL,PMN组患者PLA2R-Ab为(32.92± |
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| AbstractList | 目的 检测原发性膜性肾病(primary membranous nephropathy,PMN)患者外周血长链非编码RNA-生长停滞特异性转录本 5反义RNA1(long non-coding RNA growth arrest-specific transcript 5 antisense RNA1,lncRNA GAS5-AS1)、白细胞介素(interleukin,IL)-27表达,并分析其与患者调节性T细胞/辅助性T细胞 17(regulatory T cell/T help 17cells,Treg/Th17)平衡的关系.方法 选择 2019年 9月至 2020年 9月间河北以岭医院收治的PMN患者 94例作为PMN组.另选取同期体检健康者 96例作为对照组.收集所有研究对象外周血标本,实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)法测定血清lncRNA GAS5-AS1水平、酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)法测定IL-27、IL-17、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-10、转化生长因子β1(transforming growth factor betal,TGF-β1)水平,流式细胞仪测定外周血Treg细胞、Th17细胞比例,并计算Treg/Th17比值.Pearson法分析PMN患者lncRNA GAS5-AS1、IL-27水平与IL-17、TNF-α、IL-10、TGF-β1、Treg/Th17的相关性.结果 对照组血尿素氮(blood urea nitrogen,BUN)为(4.03±0.66)mmol/L,PMN组患者BUN为(6.52±1.05)mmol/L;对照组血尿酸(uric acid,UA)为(266.13±44.35)μmol/L,PMN组患者UA为(338.92±56.45)μmol/L;对照组胱抑素C(cystatin C,Cys C)为(0.72±0.12)mg/L,PMN组患者Cys C为(1.38±0.23)mg/L;对照组抗磷脂酶 A2受体抗体(phospholipase A2 receptor antibody,PLA2R-Ab)为(9.37±1.92)RU/mL,PMN组患者PLA2R-Ab为(32.92± |
| Abstract_FL | Objective To detect the expression of long non-coding RNA growth arrest-specific transcript 5 antisense RNA1(lncRNA GAS5-AS1)and interleukin(IL)-27 in peripheral blood of patients with primary membranous nephropathy(PMN),and to analyze their relationship with regulatory T cell/T help 17 cells(Treg/Th17)balance in patients.Methods Ninety-four PMN patients admitted to our hospi-tal from September 2019 to September 2020 were regarded as the PMN group.Another 96 cases with nor-mal physical examination during the same course were regarded as the control group.Peripheral blood samples were collected from all study subjects,real-time quantitative PCR(qRT-PCR)method was applied to determine the serum lncRNA GAS5-AS1 level,enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of IL-27,IL-17,tumor necrosis factor-α(TNF-α),IL-10,transforming growth factor betal(TGF-β1),flow cytometry was used to determine the proportions of Treg cells and Th17 cells in pe-ripheral blood,and to calculate the Treg/Th17 ratio.Pearson method was applied to analyze the correlation of lncRNA GAS5-AS1 and IL-27 levels with IL-17,TNF-α,IL-10,TGF-β1,and Treg/Th17 in PMN pa-tients.Results Compared with the control group,blood urea nitrogen(BUN)[(6.52±1.05)mmol/L vs(4.03±0.66)mmol/L],uric acid(UA)[(338.92±56.45)μmol/L vs(266.13±44.35)μmol/L],cystatin C(Cys-C)[(1.38±0.23)mg/L vs(0.72±0.12)mg/L],phospholipase A2 receptor antibody(PLA2R-Ab)[(32.92±6.19)RU/mL vs(9.37±1.92)RU/mL],24h urine protein quantification[(7.96±0.94)g/d vs(0.11±0.02)g/d],lncR-NA GAS5-AS1[(3.46±0.56)vs(1.02±0.17)],IL-27[(124.76±20.77)ng/L vs(62.71±10.44)ng/L],Th17[(4.86±0.81)%vs(0.75±0.12)%],Th17/Treg[(1.43±0.23)vs(0.11±0.02)]in PMN group increased(P<0.05),while glomerular filtration rate(GFR)[(57.47±10.24)mL·min-1·(1.73 m2)-1vs(109.22±14.85)mL·min-1·(1.73 m2)-1]and Treg levels[(3.21±0.53)%vs(6.42±1.07)%]decreased(P<0.05);Comparison of Th17/Treg,IL-17,TNF-α,IL-10,TGF-β1,lncRNA GAS5-AS1,and IL-27 levels in patients with PMN at different levels of risk,and the differences were statistically significant(P<0.05);the peripheral blood lncR-NA GAS5-AS1 of PMN patients was positively correlated with Th17/Treg,L-17,and TNF-α(r=0.632,0.554,0.572,P<0.05),and negatively correlated with IL-10(r =-0.468,P<0.05);IL-27 was positively cor-related with Th17/Treg and IL-17(r=0.581,0.602,P<0.05),and negatively correlated with IL-10 and TGF-β1(r=-0.533,-0.436,P<0.05).Conclusion Serum lncRNA GAS5-AS1 and IL-27 are highly expressed in PMN patients,both of them regulate Th17/Treg immune balance,play an important role in PMN,and are expected to become the research targets of PMN. |
| Author | 钟娇影 刘慧 刘杰 |
| AuthorAffiliation | 河北以岭医院肾病科,石家庄 050000 |
| AuthorAffiliation_xml | – name: 河北以岭医院肾病科,石家庄 050000 |
| Author_FL | Liu Jie Liu Hui Zhong Jiao-ying |
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| Keywords | 白细胞介素-27 Primary membranous nephropathy LncRNA GAS5-AS1 调节性T细胞 Treg 原发性膜性肾病 IL-27 非编码RNA-生长停滞特异性转录本5反义RNA1 Th17 辅助性T细胞17 |
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| PublicationTitle_FL | Journal of Clinical Nephrology |
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| Title | 原发性膜性肾病患者外周血lncRNA GAS5-AS1、白细胞介素-27表达及与Treg/Th17平衡的关系 |
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