Imbalance of Peripheral Blood T Helper Type 17 Responses in Patients with Vitiligo

• Published in: Iranian journal of allergy, asthma, and immunology Vol. 17; no. 2; p. 171
• Main Authors: Behfarjam, Farinaz, Mansouri, Parvine, Jadali, Zohreh
• Format: Journal Article
• Language: English
• Published: Iran Tehran University of Medical Sciences 01.04.2018
• Subjects: Adaptive Immunity; Adult; Autoimmunity; Cytokines; Enzyme-linked immunosorbent assay; Female; Gene expression; Gene Expression Profiling; Helper cells; Humans; Immune response; Inflammatory diseases; Interleukin 22; Interleukin-17 - blood; Interleukin-17 - genetics; Interleukins - blood; Interleukins - genetics; Leukocytes (mononuclear); Leukocytes, Mononuclear - metabolism; Lymphocytes T; Male; Nuclear Receptor Subfamily 1, Group F, Member 3 - genetics; Orphan receptors; Pathogenesis; Peripheral blood mononuclear cells; RNA, Messenger - metabolism; RNA-directed DNA polymerase; T lymphocyte; Th17 Cells - metabolism; Vitiligo; Vitiligo - immunology; Vitiligo - pathology; Young Adult; Autoimmunity; Vitiligo; T lymphocyte; Cytokines; Gene expression profiling
• ISSN: 1735-1502; 1735-5249

There is growing evidence to suggest that Th cells play pivotal roles in a variety of chronic inflammatory diseases, including vitiligo. However, the exact role of different subsets of Th cells in the pathogenesis of vitiligo is still a question. The purpose of present study was to determine the mRNA expression level of Th17 master transcription factor retinoic acid receptor-related orphan receptors gamma (RORɣt) and cytokine mRNA and protein expression profiles of Th17 cells. 22 patients with vitiligo and 22 normal subjects were enrolled in the study. Gene expression profiles of freshly isolated peripheral blood mononuclear cells (PBMCs) were determined by quantitative real-time reverse transcriptase PCR (qRT-PCR). Plasma concentrations of IL-17A and IL-22 were also assayed using ELISA kits. The results showed that RORɣt, IL-17A and IL-22 mRNA expression were increased in patients remarkably compared to healthy controls (p<0.05). Furthermore, plasma IL-17A and IL-22 levels were also higher in vitiligo patients versus controls (p<0.001). These data suggest that a deregulated Th17 adaptive immune response may contribute to the pathogenesis of vitiligo.