基于简化基因组测序技术的喜马拉雅旱獭遗传多样性分析

为揭示喜马拉雅旱獭（Marmota himalayana）的遗传进化机制，基于双酶切RAD（dd-RAD）测序技术，对青海省3个地区（乐都、玉树、黄南）44例喜马拉雅旱獭简化基因组测序分析，通过SNP位点刻画其遗传特性，构建系统进化树，分析种群结构特征，从基因水平分析不同地区个体之间的遗传进化关系。结果显示：3个地区喜马拉雅旱獭共获得19 279 845个SNP位点，具有丰富的遗传多样性，各地区喜马拉雅旱獭的观测杂合度（Ho）为0.236~0.269，期望杂合度（He）为0.232~0.254。3个地区中，乐都和玉树的喜马拉雅旱獭杂合过度（Ho>He），黄南地区的多态信息量最大、有效等位...

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Published in野生动物学报 Vol. 44; pp. 68 - 75
Main Authors 李优, 南新营, 赵坤钰, 李耀东
Format Journal Article
LanguageChinese
Published Editorial Department of Chinese Journal of Wildlife 01.02.2023
Subjects
喜马拉雅旱獭
简化基因组测序
Online AccessGet full text
ISSN2310-1490
DOI10.12375/ysdwxb.20230108

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Abstract 为揭示喜马拉雅旱獭（Marmota himalayana）的遗传进化机制，基于双酶切RAD（dd-RAD）测序技术，对青海省3个地区（乐都、玉树、黄南）44例喜马拉雅旱獭简化基因组测序分析，通过SNP位点刻画其遗传特性，构建系统进化树，分析种群结构特征，从基因水平分析不同地区个体之间的遗传进化关系。结果显示：3个地区喜马拉雅旱獭共获得19 279 845个SNP位点，具有丰富的遗传多样性，各地区喜马拉雅旱獭的观测杂合度（Ho）为0.236~0.269，期望杂合度（He）为0.232~0.254。3个地区中，乐都和玉树的喜马拉雅旱獭杂合过度（Ho>He），黄南地区的多态信息量最大、有效等位基因数更接近等位基因数，表明其基因纯化程度和多态性高、等位基因分布较均匀；系统发育树将黄南样品分为2个遗传分支，乐都和玉树聚为1支。研究结果可为研究喜马拉雅旱獭比较基因组学和旱獭属动物的资源合理利用提供参考依据。
AbstractList 为揭示喜马拉雅旱獭（Marmota himalayana）的遗传进化机制，基于双酶切RAD（dd-RAD）测序技术，对青海省3个地区（乐都、玉树、黄南）44例喜马拉雅旱獭简化基因组测序分析，通过SNP位点刻画其遗传特性，构建系统进化树，分析种群结构特征，从基因水平分析不同地区个体之间的遗传进化关系。结果显示：3个地区喜马拉雅旱獭共获得19 279 845个SNP位点，具有丰富的遗传多样性，各地区喜马拉雅旱獭的观测杂合度（Ho）为0.236~0.269，期望杂合度（He）为0.232~0.254。3个地区中，乐都和玉树的喜马拉雅旱獭杂合过度（Ho>He），黄南地区的多态信息量最大、有效等位基因数更接近等位基因数，表明其基因纯化程度和多态性高、等位基因分布较均匀；系统发育树将黄南样品分为2个遗传分支，乐都和玉树聚为1支。研究结果可为研究喜马拉雅旱獭比较基因组学和旱獭属动物的资源合理利用提供参考依据。
Author 南新营
李耀东
李优
赵坤钰
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Snippet 为揭示喜马拉雅旱獭（Marmota himalayana）的遗传进化机制，基于双酶切RAD（dd-RAD）测序技术，对青海省3个地区（乐都、玉树、黄南）44例喜马拉雅旱獭简化基因组测序分析，...
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SubjectTerms 喜马拉雅旱獭
简化基因组测序
Title 基于简化基因组测序技术的喜马拉雅旱獭遗传多样性分析
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