反向斑点杂交技术检测HBV YMDD基序变异 的应用和评价
【目的】 采用反向斑点杂交技术对拉米夫定治疗后的慢性乙型肝炎患者血清中HBV YMDD基序变异进行检测,并对其临床应用进行方法学评价65377;【方法】 提取242例慢性乙型肝炎患者血清中HBV DNA,经聚合酶链反应后进行膜条杂交并同测序比较分析检测结果的一致性,随后对该方法进行灵敏度和混合感染检测能力的评价65377;【结果】 242例样本检测结果有236例与测序结果相符,反向斑点杂交检测结果同测序结果的符合率达97.5%;混合型感染58例,占样品总数的24%65377;反向斑点杂交技术检测HBV YMDD基序变异的灵敏度达103 IU/mL,在病毒混合感染群体中能检测出约占10%的突变型...
Saved in:
| Published in | Zhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban Vol. 30 |
|---|---|
| Format | Journal Article |
| Language | Chinese |
| Published |
Editorial Office of Journal of Sun Yat-sen University
01.01.2009
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-3554 |
| DOI | 10.3321/j.issn:1672-3554.2009.04.015 |
Cover
| Abstract | 【目的】 采用反向斑点杂交技术对拉米夫定治疗后的慢性乙型肝炎患者血清中HBV YMDD基序变异进行检测,并对其临床应用进行方法学评价65377;【方法】 提取242例慢性乙型肝炎患者血清中HBV DNA,经聚合酶链反应后进行膜条杂交并同测序比较分析检测结果的一致性,随后对该方法进行灵敏度和混合感染检测能力的评价65377;【结果】 242例样本检测结果有236例与测序结果相符,反向斑点杂交检测结果同测序结果的符合率达97.5%;混合型感染58例,占样品总数的24%65377;反向斑点杂交技术检测HBV YMDD基序变异的灵敏度达103 IU/mL,在病毒混合感染群体中能检测出约占10%的突变型病毒株65377;【结论】 反向斑点杂交技术检测HBV YMDD基序变异具有简单65380;准确65380;经济实用的特点,具有很好的临床应用前景65377; |
|---|---|
| AbstractList | 【目的】 采用反向斑点杂交技术对拉米夫定治疗后的慢性乙型肝炎患者血清中HBV YMDD基序变异进行检测,并对其临床应用进行方法学评价65377;【方法】 提取242例慢性乙型肝炎患者血清中HBV DNA,经聚合酶链反应后进行膜条杂交并同测序比较分析检测结果的一致性,随后对该方法进行灵敏度和混合感染检测能力的评价65377;【结果】 242例样本检测结果有236例与测序结果相符,反向斑点杂交检测结果同测序结果的符合率达97.5%;混合型感染58例,占样品总数的24%65377;反向斑点杂交技术检测HBV YMDD基序变异的灵敏度达103 IU/mL,在病毒混合感染群体中能检测出约占10%的突变型病毒株65377;【结论】 反向斑点杂交技术检测HBV YMDD基序变异具有简单65380;准确65380;经济实用的特点,具有很好的临床应用前景65377; |
| BookMark | eNo9zD1Lw0AAgOEbKlhr_0MH18T7yt3FTdtqCxUXFZzCXXKRlNpI4uJWSic_iJUiIoqDDm6dRIz-nF7qzxBUnF54hncJlPpxXwOwgqBNCEarXTtK0_4aYhxbxHGojSF0bUhtiJwSKP_7IqimaaQgZA4jmLpl0DTZpbkaFzfj-fC9eBjO8ufibFDcT4unQfF63trYrx1sNxrmMTd5ZrJb8zmsze9GJp_MJy_m-uJrOpp9vC2DhVD2Ul39awXsbTZ36y2rs7PVrq93rABB6FiUEIFdgnwuhAok5z5GmlIcOiKgimtJqAipQopxqJXQSHKIXamFCyXhISIV0P79BrHsesdJdCSTUy-WkfcDcXLoyeQk8nvaI0y7mjCFeKApkq7gGnPOGHG4LzBU5BuWtG-s |
| ContentType | Journal Article |
| DBID | DOA |
| DOI | 10.3321/j.issn:1672-3554.2009.04.015 |
| DatabaseName | DOAJ Directory of Open Access Journals |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| ExternalDocumentID | oai_doaj_org_article_36e9e36b17de41a987e27766357c820b |
| GroupedDBID | ALMA_UNASSIGNED_HOLDINGS GROUPED_DOAJ |
| ID | FETCH-LOGICAL-d1005-43382931c788bda77c21e442f58d4b7ea348f4b1b670eb8e1a7029ae890a37f13 |
| IEDL.DBID | DOA |
| ISSN | 1672-3554 |
| IngestDate | Fri Oct 03 12:23:09 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Language | Chinese |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-d1005-43382931c788bda77c21e442f58d4b7ea348f4b1b670eb8e1a7029ae890a37f13 |
| OpenAccessLink | https://doaj.org/article/36e9e36b17de41a987e27766357c820b |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_36e9e36b17de41a987e27766357c820b |
| PublicationCentury | 2000 |
| PublicationDate | 2009-01-01 |
| PublicationDateYYYYMMDD | 2009-01-01 |
| PublicationDate_xml | – month: 01 year: 2009 text: 2009-01-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationTitle | Zhongshan da xue xue bao. Zhongshan daxue xuebao yixue kexue ban = Journal of Sun Yat-sen University. Yi xue ke xue ban |
| PublicationYear | 2009 |
| Publisher | Editorial Office of Journal of Sun Yat-sen University |
| Publisher_xml | – name: Editorial Office of Journal of Sun Yat-sen University |
| SSID | ssib006563249 ssib001051178 ssib002039699 ssj0002511113 ssib006703418 ssib001103819 ssib002262833 ssib051369397 ssib001186722 ssib043242460 ssib000970529 |
| Score | 1.7763302 |
| Snippet | 【目的】 采用反向斑点杂交技术对拉米夫定治疗后的慢性乙型肝炎患者血清中HBV YMDD基序变异进行检测,并对其临床应用进行方法学评价65377;【方法】 提取242例慢性乙型肝炎患者... |
| SourceID | doaj |
| SourceType | Open Website |
| SubjectTerms | 乙型肝炎病毒(HBV) 反向斑点杂交 突变 耐药 |
| Title | 反向斑点杂交技术检测HBV YMDD基序变异 的应用和评价 |
| URI | https://doaj.org/article/36e9e36b17de41a987e27766357c820b |
| Volume | 30 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV07S8RAEF7EQmzEJ765wjaafWQ3a-eTQzgrFa2ObLLBSkW0sToOKx-cioiIYqGFnZWIpz_H5PwZ7iRBt7OxTWBDhsnM92WG70NogiqPcIWl4zMVOkxw7SgScUdGITP43RNRNi6oLPPyKlta99Ytqy_YCcvlgfPATVGupabmOBFphgNDkTURAvqkCE33UlB9XV9aZCoHDgJGWBbxMcDCEq7CoAtuC5mBrpslZEdcKrkN1AknvgWMDAqiNnHh5sNhv1UeZO4I-yUeHqZc0mJeBz0CgD3OvJuxea4DTb8DTQCFpgTWjKAATP_cKmQ12aQL7r2Wt0DWBBe7UVeBXkszedR6UNvBZi_qqBTz-T60kDROk7Pz9Oq8VX9L7-qfzcf0qJbePqcPtfTluDy7VtqozM8n982k2Uga18lHvdS6OUyal63Lp-Ti5Ov58PP9tR-tLi6szJWdwqjBiTAomTLDcw1swKHh0yoKhAgJ1oyR2PMjpoQOKPNjprAyYdLK1zgQLpGB9qUbUBFjOoDat7a39CAqYU2ICrCMtZIMwxaMVtzXMZFCCRVHQ2gWXr-6k2txVEEdO7tgcqZa5Ez1r5wZ_o9DRlBnPlmC3zGjqH1vd1-PGYCyp8azXPwG49Pfeg |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%8F%8D%E5%90%91%E6%96%91%E7%82%B9%E6%9D%82%E4%BA%A4%E6%8A%80%E6%9C%AF%E6%A3%80%E6%B5%8BHBV+YMDD%E5%9F%BA%E5%BA%8F%E5%8F%98%E5%BC%82+%E7%9A%84%E5%BA%94%E7%94%A8%E5%92%8C%E8%AF%84%E4%BB%B7&rft.jtitle=Zhongshan+da+xue+xue+bao.+Zhongshan+daxue+xuebao+yixue+kexue+ban+%3D+Journal+of+Sun+Yat-sen+University.+Yi+xue+ke+xue+ban&rft.date=2009-01-01&rft.pub=Editorial+Office+of+Journal+of+Sun+Yat-sen+University&rft.issn=1672-3554&rft.volume=30&rft_id=info:doi/10.3321%2Fj.issn%3A1672-3554.2009.04.015&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_36e9e36b17de41a987e27766357c820b |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1672-3554&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1672-3554&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1672-3554&client=summon |