The effect of green robust coffee gel (coffea canephora) to collagen density on gingival wound healing (an in-vivo study in wistar rats)

• Published in: Journal of dentomaxillofacial science (Makassar) Vol. 8; no. 1
• Main Authors: Suryadewi, Ni-Putu I., Utomo, Rinaldi B., Bramanti, Indra
• Format: Journal Article
• Language: English
• Published: 01.04.2023
• ISSN: 2503-0817; 2503-0825; 2503-0825
• DOI: 10.15562/jdmfs.v8i1.1435

Objective: This study aims to determine the effect of robusta green coffee gel on the collagen density in Wistar rats. Material and Methods: The subjects were 45 Wistar rats, each wounded with a 3 mm punch biopsy in the mandibular incisor gingiva. The rats were then divided into 5 groups, namely the positive control group (Povidone-iodine 10%), negative control (gel base), and 3 treatment groups (10%, 20%, and 40% concentration of Robusta green coffee gel). The intervention was carried out twice a day. A total of 3 mice in each group were then sacrificed on the 5th and 10th days to see the collagen density using the Mallory Trichrome staining technique. Collagen density was observed using a microscope with 400x magnification in 5 fields of view. Kruskal Wallis test was used for data processing. Results: There was no difference in collagen density regardless of the coffee gel concentration at each observation time, with p>0.05. Conclusion: There was no effect on the times of observation or dose concentration of robusta coffee gel on collagen density on gingival wound healing in Wistar rats.