在线净化/固相萃取-高效液相色谱法测定柑橘中多菌灵痕量残留量
目的通过在线净化/固相萃取-高效液相色谱技术建立测定柑橘中痕量多菌灵的方法。方法 样品在固相萃取小柱Cyclone-MCX中进行净化和富集后,通过阀将固相萃取小柱切换至分析流路,使待测物从富集柱冲洗至分析柱PAⅡC18中,采用梯度洗脱方式完成多菌灵的分离和检测。结果 多菌灵在1~20μg/L范围内呈线性相关,线性方程为Y=0.0921X-0.0016,相关系数r=0.9995;检出限为0.1μg/L(S/N=3);柑橘中3个水平添加回收率(n=6)在86.5%~96.7%;6次加标回收相对标准偏差为2.34%~3.21%。结论 所建方法简便、快速、准确、重现性好,能够满足柑橘中多菌灵痕量残留量...
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| Published in | 食品安全质量检测学报 no. 11; pp. 3691 - 3698 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖南李文食品有限公司技术研发及设计中心，邵阳 422000%邵阳学院生物与化学工程系，邵阳 422000
2014
湖南李文食品有限公司技术研发及设计中心，邵阳 422000 邵阳学院生物与化学工程系，邵阳 422000 豆制品加工技术湖南省应用基础研究基地，邵阳 422000 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的通过在线净化/固相萃取-高效液相色谱技术建立测定柑橘中痕量多菌灵的方法。方法 样品在固相萃取小柱Cyclone-MCX中进行净化和富集后,通过阀将固相萃取小柱切换至分析流路,使待测物从富集柱冲洗至分析柱PAⅡC18中,采用梯度洗脱方式完成多菌灵的分离和检测。结果 多菌灵在1~20μg/L范围内呈线性相关,线性方程为Y=0.0921X-0.0016,相关系数r=0.9995;检出限为0.1μg/L(S/N=3);柑橘中3个水平添加回收率(n=6)在86.5%~96.7%;6次加标回收相对标准偏差为2.34%~3.21%。结论 所建方法简便、快速、准确、重现性好,能够满足柑橘中多菌灵痕量残留量的测定。 |
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| AbstractList | 目的通过在线净化/固相萃取-高效液相色谱技术建立测定柑橘中痕量多菌灵的方法。方法 样品在固相萃取小柱Cyclone-MCX中进行净化和富集后,通过阀将固相萃取小柱切换至分析流路,使待测物从富集柱冲洗至分析柱PAⅡC18中,采用梯度洗脱方式完成多菌灵的分离和检测。结果 多菌灵在1~20μg/L范围内呈线性相关,线性方程为Y=0.0921X-0.0016,相关系数r=0.9995;检出限为0.1μg/L(S/N=3);柑橘中3个水平添加回收率(n=6)在86.5%~96.7%;6次加标回收相对标准偏差为2.34%~3.21%。结论 所建方法简便、快速、准确、重现性好,能够满足柑橘中多菌灵痕量残留量的测定。 目的：通过在线净化/固相萃取-高效液相色谱技术建立测定柑橘中痕量多菌灵的方法。方法样品在固相萃取小柱 Cyclone-MCX 中进行净化和富集后,通过阀将固相萃取小柱切换至分析流路,使待测物从富集柱冲洗至分析柱PAⅡC18中,采用梯度洗脱方式完成多菌灵的分离和检测。结果多菌灵在1~20μg/L范围内呈线性相关,线性方程为Y=0.0921X-0.0016,相关系数r=0.9995；检出限为0.1μg/L(S/N=3)；柑橘中3个水平添加回收率(n=6)在86.5%~96.7%；6次加标回收相对标准偏差为2.34%~3.21%。结论所建方法简便、快速、准确、重现性好,能够满足柑橘中多菌灵痕量残留量的测定。 |
| Abstract_FL | Objective Toestablish a method for determination of carbendazim in citrus by high perfor-mance liquid chromatography with on-line clean-up and solid phase extraction.Methods After the samples were purified and enriched in the solid phase extraction column Cyclone-MCX, the solid phase extraction column switch to the analysis flow path by switching valve, then let the target analytes flush from the enrich-ment column to the analytical column PAⅡC18, accomplished separation and detection of carbendazim by using the method of gradient elution.Results The linear range was 1~20 μg/L, a linear regression equation was fit-ted as follows:Y=0.0921X-0.0016 with a correlation coefficient of 0.9995. The detection limit was 0.1μg/L. Average recovery at various levels were 86.5%~96.7% with RSD 2.34%~3.21% (n=6).Conclusion The es-tablished method is simple, rapid, accurate and credible, and can be applied in rapid determination of trace carbendazim in citrus with satisfactory results. |
| Author | 周晓洁 赵良忠 夏湘 王丽 |
| AuthorAffiliation | 邵阳学院生物与化学工程系 湖南李文食品有限公司技术研发及设计中心 豆制品加工技术湖南省应用基础研究基地 |
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| Author_FL | ZHAO Liang-Zhong ZHOU Xiao-Jie XIA Xiang WANG Li |
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| DocumentTitle_FL | Determination of trace carbendazim in citrus by high performance liquid chromatography with on-line clean-up and solid phase extraction |
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| Keywords | carbendazim 在线净化/固相萃取 high performance liquid chromatography on-line clean-up and solid phase extraction 高效液相色谱法 柑橘 多菌灵 citrus |
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| Publisher | 湖南李文食品有限公司技术研发及设计中心，邵阳 422000%邵阳学院生物与化学工程系，邵阳 422000 湖南李文食品有限公司技术研发及设计中心，邵阳 422000 邵阳学院生物与化学工程系，邵阳 422000 豆制品加工技术湖南省应用基础研究基地，邵阳 422000 |
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| Title | 在线净化/固相萃取-高效液相色谱法测定柑橘中多菌灵痕量残留量 |
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