Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha
The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite r...
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| Published in | PloS one Vol. 13; no. 10; p. e0204964 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
04.10.2018
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1932-6203 1932-6203 |
| DOI | 10.1371/journal.pone.0204964 |
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| Abstract | The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha. |
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| AbstractList | The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3xHA, 4xMyc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha. The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha.The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha. The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha. The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M . polymorpha . The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the att R2 site is replaced with att L1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M . polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M . polymorpha . |
| Audience | Academic |
| Author | Nishimura, Mikio Nakagawa, Tsuyoshi Mano, Shoji Ishida, Sakiko Hikino, Kazumi Nishihama, Ryuichi Yamato, Katsuyuki T. Kondo, Maki Kohchi, Takayuki |
| AuthorAffiliation | 4 Spectrography and Bioimaging Facility, NIBB Core Research Facilities, National Institute for Basic Biology, Okazaki, Japan 2 Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan 3 Graduate School of Biostudies, Kyoto University, Kyoto, Japan 1 Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan 6 Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Matsue, Japan CSMCRI, INDIA 5 Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama, Japan |
| AuthorAffiliation_xml | – name: 4 Spectrography and Bioimaging Facility, NIBB Core Research Facilities, National Institute for Basic Biology, Okazaki, Japan – name: 3 Graduate School of Biostudies, Kyoto University, Kyoto, Japan – name: 5 Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama, Japan – name: CSMCRI, INDIA – name: 1 Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan – name: 2 Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan – name: 6 Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Matsue, Japan |
| Author_xml | – sequence: 1 givenname: Shoji orcidid: 0000-0003-1323-3308 surname: Mano fullname: Mano, Shoji – sequence: 2 givenname: Ryuichi surname: Nishihama fullname: Nishihama, Ryuichi – sequence: 3 givenname: Sakiko surname: Ishida fullname: Ishida, Sakiko – sequence: 4 givenname: Kazumi surname: Hikino fullname: Hikino, Kazumi – sequence: 5 givenname: Maki surname: Kondo fullname: Kondo, Maki – sequence: 6 givenname: Mikio surname: Nishimura fullname: Nishimura, Mikio – sequence: 7 givenname: Katsuyuki T. surname: Yamato fullname: Yamato, Katsuyuki T. – sequence: 8 givenname: Takayuki surname: Kohchi fullname: Kohchi, Takayuki – sequence: 9 givenname: Tsuyoshi surname: Nakagawa fullname: Nakagawa, Tsuyoshi |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30286137$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_cub_2024_01_013 crossref_primary_10_2139_ssrn_3936040 crossref_primary_10_1093_pcp_pcz029 crossref_primary_10_1242_dev_200951 crossref_primary_10_3389_fcell_2022_883491 crossref_primary_10_1007_s10265_020_01180_5 crossref_primary_10_1371_journal_pone_0221164 crossref_primary_10_1146_annurev_arplant_082520_094256 crossref_primary_10_1016_j_celrep_2022_110975 crossref_primary_10_1093_plcell_koac219 crossref_primary_10_3389_fpls_2020_569194 |
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| Copyright | COPYRIGHT 2018 Public Library of Science 2018 Mano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2018 Mano et al 2018 Mano et al |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Current address: Department of Biology, Faculty of Science and Engineering, Konan University, Kobe, Japan Competing Interests: The authors have declared that no competing interests exist. |
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| SubjectTerms | Aquatic plants Assembly Biology Biology and Life Sciences Cloning Cloning vectors Deoxyribonucleic acid DNA Engineering and Technology Gene expression Genetic aspects Genetic engineering Genomics Liverworts Marchantia polymorpha Myc protein Pests Promoters (Genetics) Proteins Recombination Research and analysis methods Tags |
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| Title | Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha |
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