Characterization of Mammalian Selenoproteomes

• Published in: Science (American Association for the Advancement of Science) Vol. 300; no. 5624; pp. 1439 - 1443
• Main Authors: Kryukov, Gregory V., Castellano, Sergi, Novoselov, Sergey V., Lobanov, Alexey V., Zehtab, Omid, Guigó, Roderic, Gladyshev, Vadim N.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 30.05.2003; The American Association for the Advancement of Science
• Subjects: Amino Acid Sequence; Analysis; Animals; Base Sequence; Biological and medical sciences; Coding; Codon; Codon, Terminator; Codons; Computational Biology; DNA Transposable Elements; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Genetic aspects; Genetic code; Genetic research; Genetics; Genome, Human; Genomes; Human genome; Humans; Mammals; Messenger RNA; Mice; Molecular and cellular biology; Molecular Sequence Data; Observation; Open Reading Frames; Proteins; Proteins - chemistry; Proteins - genetics; Proteome; Proteomics; Rats; Selenium; Selenocysteine - chemistry; Selenocysteine - genetics; Selenoproteins; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Software; United States; Genetics
• ISSN: 0036-8075; 1095-9203; 1095-9203
• DOI: 10.1126/science.1083516

In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are misannotated. We identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs. The human selenoproteome consists of 25 selenoproteins.