Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing - Guo, Tao; Feng, Yi-Li; Xiao, Jing-Jing; Liu, Qian; Sun, Xiu-Na; Xiang, Ji-Feng; Kong, Na; Liu, Si-Cheng; Chen, Guo-Qiao; Wang, Yue; Dong, Meng-Meng; Cai, Zhen; et al. (Genome Biology, 2018)
Keywords: Accurate NHEJ; Animal Genetics and Genomics; Animals; Base Sequence; Bioinformatics; Biomedical and Life Sciences; Clonal deletion; CRISPR; CRISPR-Cas systems; CRISPR-Cas Systems - genetics; CRISPR/Cas9; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA damage; DNA End-Joining Repair - genetics; DNA repair; DNA-Binding Proteins - metabolism; Efficiency; Evolutionary Biology; Gene Editing; Gene Knockout Techniques; Genes; Genetic engineering; Genome; Genome editing; Genomes; gRNA; HEK293 Cells; Human Genetics; Humans; Insights from Genome Editing; Knockout; Life Sciences; Liver; Liver - metabolism; Mice; Microbial Genetics and Genomics; Muscular dystrophy; Mutagenesis, Insertional - genetics; mutagens; Mutation; Non-homologous end joining; Paired gRNAs; Plant Genetics and Genomics; Precise deletion; Reproducibility of Results; Sequence Deletion; Stem cells; Templated insertions; Templated insertions; Precise deletion; Accurate NHEJ; CRISPR/Cas9; Targeted in-frame deletion; Paired gRNAs; Genome editing; Knockout