Transcriptional Regulatory Networks in Saccharomyces cerevisiae

• Published in: Science (American Association for the Advancement of Science) Vol. 298; no. 5594; pp. 799 - 804
• Main Authors: Lee, Tong Ihn, Rinaldi, Nicola J., Robert, François, Odom, Duncan T., Bar-Joseph, Ziv, Gerber, Georg K., Hannett, Nancy M., Harbison, Christopher T., Thompson, Craig M., Simon, Itamar, Zeitlinger, Julia, Jennings, Ezra G., Murray, Heather L., Gordon, D. Benjamin, Ren, Bing, Wyrick, John J., Tagne, Jean-Bosco, Volkert, Thomas L., Fraenkel, Ernest, Gifford, David K., Young, Richard A.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 25.10.2002; The American Association for the Advancement of Science
• Subjects: Algorithms; Biological and medical sciences; Brewer's yeast; Cell Cycle; Cell cycle proteins; Cellular biology; Coding; Computational Biology; Control loops; cytology; DNA, Fungal; DNA, Fungal - genetics; DNA, Fungal - metabolism; Feedback, Physiological; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene expression regulation; Gene Expression Regulation, Fungal; Genes; Genes, Fungal; Genetic regulation; genetics; genome; Genome, Fungal; Genomes; Genomics; growth & development; metabolism; Models, Genetic; Molecular and cellular biology; Molecular genetics; Networks; P values; Promoter regions; Promoter Regions, Genetic; Protein Binding; Quality Control; Regulator genes; Research Design; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; transcription (genetics); transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Transcriptional regulatory elements; Yeast; Yeasts; Fungi; Regulation(control); Yeast; Transcription; Ascomycetes; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0036-8075; 1095-9203; 1095-9203
• DOI: 10.1126/science.1075090

We have determined how most of the transcriptional regulators encoded in the eukaryote Saccharomyces cerevisiae associate with genes across the genome in living cells. Just as maps of metabolic networks describe the potential pathways that may be used by a cell to accomplish metabolic processes, this network of regulator-gene interactions describes potential pathways yeast cells can use to regulate global gene expression programs. We use this information to identify network motifs, the simplest units of network architecture, and demonstrate that an automated process can use motifs to assemble a transcriptional regulatory network structure. Our results reveal that eukaryotic cellular functions are highly connected through networks of transcriptional regulators that regulate other transcriptional regulators.