Comparative analysis of blood vessel density in unchanged bone marrow, acute and chronic myeloid leukemia

• Published in: Medicinski pregled Vol. 78; no. 1-2; pp. 24 - 28
• Main Authors: Stanic, David, Ilic-Sabo, Jelena, Lakic, Tanja, Percic, Ivanka, Dokic, Marina, Stojsic, Nikola
• Format: Journal Article
• Language: English
• Published: 2025
• ISSN: 0025-8105; 1820-7383
• DOI: 10.2298/MPNS2502024S

Introduction. Acute myeloid leukemia and chronic myeloid leukemia are malignant blood cell disorders characterized by increased angiogenesis. This study aimed to evaluate blood vessel density in bone marrow tissue of patients with acute and chronic myeloid leukemia and to determine whether there is a statistically significant difference compared to a control group. Material and Methods. This retrospective study included 90 bone marrow biopsy samples, categorized into three groups: control, acute myeloid leukemia, and chronic myeloid leukemia. Pathohistological diagnoses were retrieved from the records of the Center for Pathology and Histology of the Clinical Center of Vojvodina. Samples were processed using standard histological and immunohistochemical techniques, examined microscopically, and photographed. Blood vessel density was quantified using ImageJ program, analyzing five microphotographs per sample, with results expressed as percentages. The mean value was calculated for each subject. Data were analyzed statistically and presented numerically, graphically, and in tables. Results. The mean blood vessel density was 1.85% (?}0.56%) in the control group, 3.72 (?}1.67%) in the acute myeloid leukemia group, and 5.42% (?}2.28%) in the chronic myeloid leukemia group. A statistically significant difference in blood vessel density was observed among the groups. Conclusion. The statistically significant difference in blood vessel density between the groups underscores the role of angiogenesis in the pathogenesis of these malignancies and suggests its potential relevance in diagnosis and therapeutic strategies.