Age-related changes in DNA methylation levels at CpG sites in bull spermatozoa and in vitro fertilization-derived blastocyst-stage embryos revealed by combined bisulfite restriction analysis - KANEDA, Masahiro; ADACHI, Hiromichi; TAKEDA, Kumiko; KOBAYASHI, Eiji; HOSHINO, Yoichiro; IWAO, Ken; AKAGI, Satoshi; NISHINO, Kagetomo; IMAI, Akira; WATANABE, Shinya (Journal of Reproduction and Development, 2019)
Keywords: Age; Age Factors; Age-related changes; Aging - genetics; Animals; Bisulfite; Blastocyst - metabolism; Bull spermatozoa; Cattle; Cells, Cultured; Combinatorial Chemistry Techniques - methods; Combined bisulfite restriction analysis (COBRA); CpG islands; CpG Islands - genetics; Cryopreservation; Deoxyribonucleic acid; DNA; DNA methylation; DNA Methylation - genetics; DNA microarrays; Embryo Culture Techniques; Embryo, Mammalian; Embryonic Development - genetics; Embryos; Female; Fertility; Fertilization in Vitro - veterinary; Genomes; Humans; In vitro fertilization; In vitro fertilization (IVF) embryos; Male; Oligonucleotide Array Sequence Analysis - methods; Original; Restriction Mapping - methods; Semen; Sequence Analysis, DNA - methods; Sperm; Spermatozoa - metabolism; Sulfites - chemistry; Age-related changes; DNA methylation; Bull spermatozoa; Combined bisulfite restriction analysis (COBRA); In vitro fertilization (IVF) embryos