Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno‐associated virus capsid and promoter

In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particu...

Full description

Saved in:
Bibliographic Details
Published inEMBO molecular medicine Vol. 6; no. 9; pp. 1175 - 1190
Main Authors Cronin, Therese, Vandenberghe, Luk H, Hantz, Péter, Juttner, Josephine, Reimann, Andreas, Kacsó, Ágota–Enikő, Huckfeldt, Rachel M, Busskamp, Volker, Kohler, Hubertus, Lagali, Pamela S, Roska, Botond, Bennett, Jean
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 01.09.2014
EMBO Press
Wiley Open Access
Blackwell Publishing Ltd
Springer Nature
Subjects
adeno‐associated virus
Animals
Binding sites
Bioengineering
Bipolar cells
Blindness
capsid library
Clinical trials
Dependovirus - genetics
EMBO16
EMBO27
Experiments
Ganglion cells
Gene expression
Gene therapy
Genetic Vectors
HEK293 Cells
Heparan sulfate
Humans
Imaging
Libraries
Life Sciences
Light
Mice
Mice, Inbred C57BL
multi‐electrode array
Mutagenesis
Mutagenesis, Site-Directed
Neurobiology
Neurons and Cognition
optogenetics
Photoreceptors
promoter optimization
Promoter Regions, Genetic
Research Article
Retina
Retinal Bipolar Cells - virology
Transduction, Genetic - methods
Vectors (Biology)
capsid library
adeno‐associated virus
promoter optimization
multi‐electrode array
optogenetics
Neuroscience
adeno-associated virus
promoter optimization Subject Categories Genetics
Gene Therapy & Genetic Disease
multi-electrode array
Online AccessGet full text
ISSN1757-4676
1757-4684
1757-4684
DOI10.15252/emmm.201404077

Cover

Abstract In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON‐bipolar cells light‐sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON‐bipolar cells. This evokes high‐frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1 , mice. Our vector is a promising vehicle for further development toward potential clinical use. Synopsis An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice. A synthetic AAV capsid and modified bipolar‐cell specific promoter were developed to enhance transgene expression in retinal bipolar cells. The new virus transduced at least 59% of ON‐bipolar cells in mouse retina. In the blind rd1 mouse the virus was used to drive expression of optogenetic channels at levels high enough to elicit strong and robust spiking responses from the ganglion cells. This new virus‐promoter combination is thus presented as a candidate vector for clinical intervention in advanced forms of retinal degeneration. Graphical Abstract An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice.
AbstractList In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON-bipolar cells light-sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON-bipolar cells. This evokes high-frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.
Abstract In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON‐bipolar cells light‐sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON‐bipolar cells. This evokes high‐frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.
In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON-bipolar cells light-sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON-bipolar cells. This evokes high-frequency (∼100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.
In this report, we describe the development of a modified adeno‐associated virus ( AAV ) capsid and promoter for transduction of retinal ON ‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON ‐bipolar cells light‐sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON ‐bipolar cells. This evokes high‐frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1 , mice. Our vector is a promising vehicle for further development toward potential clinical use. image An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON ‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice. A synthetic AAV capsid and modified bipolar‐cell specific promoter were developed to enhance transgene expression in retinal bipolar cells. The new virus transduced at least 59% of ON ‐bipolar cells in mouse retina. In the blind rd1 mouse the virus was used to drive expression of optogenetic channels at levels high enough to elicit strong and robust spiking responses from the ganglion cells. This new virus‐promoter combination is thus presented as a candidate vector for clinical intervention in advanced forms of retinal degeneration.
In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON-bipolar cells light-sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON-bipolar cells. This evokes high-frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON-bipolar cells light-sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON-bipolar cells. This evokes high-frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.
In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON‐bipolar cells light‐sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON‐bipolar cells. This evokes high‐frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1 , mice. Our vector is a promising vehicle for further development toward potential clinical use. Synopsis An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice. A synthetic AAV capsid and modified bipolar‐cell specific promoter were developed to enhance transgene expression in retinal bipolar cells. The new virus transduced at least 59% of ON‐bipolar cells in mouse retina. In the blind rd1 mouse the virus was used to drive expression of optogenetic channels at levels high enough to elicit strong and robust spiking responses from the ganglion cells. This new virus‐promoter combination is thus presented as a candidate vector for clinical intervention in advanced forms of retinal degeneration. Graphical Abstract An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice.
In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON‐bipolar cells light‐sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON‐bipolar cells. This evokes high‐frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use.
In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The bipolar cells, which are post‐synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced forms of blindness involves using optogenetic molecules to render ON‐bipolar cells light‐sensitive. Currently, delivery of adequate levels of gene expression is a limiting step for this approach. The synthetic AAV capsid and promoter described here achieves high level of optogenetic transgene expression in ON‐bipolar cells. This evokes high‐frequency (~100 Hz) spiking responses in ganglion cells of previously blind, rd1, mice. Our vector is a promising vehicle for further development toward potential clinical use. Synopsis An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice. A synthetic AAV capsid and modified bipolar‐cell specific promoter were developed to enhance transgene expression in retinal bipolar cells. The new virus transduced at least 59% of ON‐bipolar cells in mouse retina. In the blind rd1 mouse the virus was used to drive expression of optogenetic channels at levels high enough to elicit strong and robust spiking responses from the ganglion cells. This new virus‐promoter combination is thus presented as a candidate vector for clinical intervention in advanced forms of retinal degeneration. An engineered genetically modified adeno‐associated virus is shown here to efficiently and specifically drive the optogenetic molecule channelrhodopsin‐2 in ON‐bipolar cells, rendering them light sensitive and restoring retinal function in blind rd1 mice.
Author Kohler, Hubertus
Kacsó, Ágota–Enikő
Vandenberghe, Luk H
Roska, Botond
Hantz, Péter
Huckfeldt, Rachel M
Juttner, Josephine
Reimann, Andreas
Bennett, Jean
Lagali, Pamela S
Cronin, Therese
Busskamp, Volker
Author_xml – sequence: 1
  givenname: Therese
  surname: Cronin
  fullname: Cronin, Therese
  email: therese.cronin@fmi.ch
  organization: F.M. Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania, Friedrich Miescher Institute for Biomedical Research
– sequence: 2
  givenname: Luk H
  surname: Vandenberghe
  fullname: Vandenberghe, Luk H
  organization: F.M. Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School
– sequence: 3
  givenname: Péter
  surname: Hantz
  fullname: Hantz, Péter
  organization: Friedrich Miescher Institute for Biomedical Research
– sequence: 4
  givenname: Josephine
  surname: Juttner
  fullname: Juttner, Josephine
  organization: Friedrich Miescher Institute for Biomedical Research
– sequence: 5
  givenname: Andreas
  surname: Reimann
  fullname: Reimann, Andreas
  organization: Friedrich Miescher Institute for Biomedical Research
– sequence: 6
  givenname: Ágota–Enikő
  surname: Kacsó
  fullname: Kacsó, Ágota–Enikő
  organization: Faculty of Physics, Babes‐Bolyai University
– sequence: 7
  givenname: Rachel M
  surname: Huckfeldt
  fullname: Huckfeldt, Rachel M
  organization: F.M. Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania
– sequence: 8
  givenname: Volker
  surname: Busskamp
  fullname: Busskamp, Volker
  organization: Friedrich Miescher Institute for Biomedical Research, Genetics Department, Harvard Medical School
– sequence: 9
  givenname: Hubertus
  surname: Kohler
  fullname: Kohler, Hubertus
  organization: Friedrich Miescher Institute for Biomedical Research
– sequence: 10
  givenname: Pamela S
  surname: Lagali
  fullname: Lagali, Pamela S
  organization: Friedrich Miescher Institute for Biomedical Research, Regenerative Medicine Program, Ottawa Hospital Research Institute
– sequence: 11
  givenname: Botond
  surname: Roska
  fullname: Roska, Botond
  organization: Friedrich Miescher Institute for Biomedical Research
– sequence: 12
  givenname: Jean
  surname: Bennett
  fullname: Bennett, Jean
  email: jebennet@mail.med.upenn.edu
  organization: F.M. Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25092770$$D View this record in MEDLINE/PubMed
https://inserm.hal.science/inserm-02894121$$DView record in HAL
BookMark eNqFUk1v1DAQjVAR_YAzNxSJCwe2tb12nHCoVFULrdSKC5ytiT3ZdZXYwU622hs3rvxGfgnublnalaAnWzPvvfl4c5jtOe8wy15TckwFE-wEu647ZoRywomUz7IDKoWc8KLke9u_LPazwxhvCClEQcsX2T4TpGJSkoPsx6xprLbohnwI4KIZ9WC9y8GZ3PeDn6PDweo8DrYbW1jnfJOHFHTQ5rXtfQsh19i2Ma9XOeRx5YbFmgMGnf_1_SfE6LWFAU2-tGGMuYY-WrOu0Qff-QHDy-x5A23EV_fvUfb14-zL-cXk6vOny_Ozq4mWgsmJ4VQYQUADM4LVsmokrQyZ1gTMFJluWCUoE1iBJpyWgMYIboBhPS2hLsrpUXa50TUeblQfbAdhpTxYtQ74MFcQUvMtKuSN5iUiUql5IyjwStQNKVjJhOZTmbTIRmt0PaxuoW23gpSotT_qzh-19SdRTjeUfqw7NDrtPUD7qI_HGWcXau6XitNKlgVPAu83Aosd2sXZlbIuYugUYWXFKaNLmuDv7usF_23EOKjOxjuzwKEfY2pSVOkk2FQk6Nsd6I0fQzI5KsYqQpks-J3gm4cDbFv4c1EJIDYAHXyMARul7bC-mzSPbf-zmZMd3tO7_LBh3NoWV0_B1ez6-voh-d67mHhujuHvtP-q9xujahQa
CitedBy_id crossref_primary_10_1016_j_exer_2021_108553
crossref_primary_10_1101_cshperspect_a041304
crossref_primary_10_1146_annurev_vision_102016_061413
crossref_primary_10_1089_hum_2022_079
crossref_primary_10_1016_j_ymthe_2018_02_027
crossref_primary_10_1101_cshperspect_a041660
crossref_primary_10_1007_s10162_022_00866_y
crossref_primary_10_1016_j_cell_2020_08_013
crossref_primary_10_1089_hum_2022_109
crossref_primary_10_1097_WNO_0000000000000476
crossref_primary_10_3390_genes10090654
crossref_primary_10_1089_hum_2024_126
crossref_primary_10_1038_s42003_022_04016_1
crossref_primary_10_3389_fnsys_2016_00074
crossref_primary_10_3389_fddev_2022_899260
crossref_primary_10_1089_hum_2021_278
crossref_primary_10_1038_s43586_022_00136_4
crossref_primary_10_3389_fninf_2017_00070
crossref_primary_10_3389_fnins_2017_00521
crossref_primary_10_1016_j_ebiom_2021_103360
crossref_primary_10_1167_tvst_11_5_24
crossref_primary_10_1038_s41467_025_55882_2
crossref_primary_10_1088_1741_2552_aadd55
crossref_primary_10_1128_JVI_02213_17
crossref_primary_10_3389_fnins_2016_00551
crossref_primary_10_2174_1566523220666200211111326
crossref_primary_10_1038_nn_4600
crossref_primary_10_1007_s11055_020_00911_4
crossref_primary_10_1016_j_neuron_2024_08_015
crossref_primary_10_1016_j_ymthe_2017_03_008
crossref_primary_10_1051_medsci_20153104015
crossref_primary_10_3389_fncel_2015_00486
crossref_primary_10_1016_j_omtm_2019_01_008
crossref_primary_10_1126_science_aaz5887
crossref_primary_10_1016_j_addr_2020_06_011
crossref_primary_10_1016_j_neuron_2018_06_001
crossref_primary_10_1186_s12967_022_03738_4
crossref_primary_10_1016_j_cub_2022_12_026
crossref_primary_10_1038_s41434_023_00436_8
crossref_primary_10_1134_S0006297919050031
crossref_primary_10_1038_mtm_2015_16
crossref_primary_10_1016_j_crmeth_2022_100268
crossref_primary_10_1016_j_preteyeres_2018_11_001
crossref_primary_10_1038_s41593_019_0431_2
crossref_primary_10_1016_j_omtm_2021_01_011
crossref_primary_10_1038_s41434_021_00306_1
crossref_primary_10_1038_gt_2016_54
crossref_primary_10_1038_s41467_018_08243_1
crossref_primary_10_1097_WCO_0000000000000168
crossref_primary_10_1016_j_cub_2023_08_044
crossref_primary_10_1016_j_omtm_2017_12_002
crossref_primary_10_1016_j_tibtech_2015_09_008
crossref_primary_10_1016_j_omtm_2023_09_003
crossref_primary_10_1002_bit_26031
crossref_primary_10_1152_physrev_00035_2019
crossref_primary_10_1016_j_exer_2018_09_003
crossref_primary_10_1097_ICU_0000000000000140
crossref_primary_10_3389_fnins_2024_1415575
crossref_primary_10_1073_pnas_1701589114
crossref_primary_10_1089_hum_2020_038
crossref_primary_10_1080_14712598_2017_1271870
crossref_primary_10_1038_gt_2016_42
crossref_primary_10_1007_s00018_020_03597_6
crossref_primary_10_1016_j_tips_2021_03_004
crossref_primary_10_1007_s11055_019_00714_2
crossref_primary_10_1113_JP282076
crossref_primary_10_1371_journal_pbio_1002143
crossref_primary_10_1016_j_omtm_2018_01_008
crossref_primary_10_1080_14712598_2021_1865303
crossref_primary_10_1152_jn_00087_2016
crossref_primary_10_1016_j_neuron_2021_07_008
crossref_primary_10_1089_hum_2016_040
crossref_primary_10_1016_j_lfs_2017_11_010
crossref_primary_10_1016_j_conb_2015_01_004
crossref_primary_10_1002_SMMD_20230026
crossref_primary_10_1016_j_lfs_2016_02_057
crossref_primary_10_3389_fnins_2017_00161
crossref_primary_10_1038_gt_2017_85
crossref_primary_10_1016_j_omtm_2023_05_011
crossref_primary_10_1016_j_preteyeres_2022_101089
crossref_primary_10_1136_bjophthalmol_2015_308329
crossref_primary_10_1016_j_omtm_2020_03_003
crossref_primary_10_3389_fddev_2023_934394
crossref_primary_10_1016_j_omtm_2022_03_003
crossref_primary_10_1111_jnc_14124
crossref_primary_10_15252_emmm_201505699
crossref_primary_10_3389_fncel_2018_00316
crossref_primary_10_34133_research_0291
crossref_primary_10_1016_j_ymthe_2025_01_030
crossref_primary_10_1038_s41467_018_05059_x
crossref_primary_10_1038_s41551_021_00829_3
crossref_primary_10_1016_j_cub_2015_07_029
crossref_primary_10_1038_s41598_023_49112_2
crossref_primary_10_1097_IIO_0000000000000386
crossref_primary_10_3389_fnins_2020_570909
crossref_primary_10_1016_j_ymthe_2019_04_002
crossref_primary_10_1016_j_conb_2015_01_018
crossref_primary_10_1097_IIO_0000000000000416
crossref_primary_10_1101_cshperspect_a041291
crossref_primary_10_1109_TBCAS_2019_2951298
crossref_primary_10_1167_tvst_11_1_18
crossref_primary_10_3389_fnins_2017_00174
crossref_primary_10_1155_2021_4014797
crossref_primary_10_3390_ijms222111515
crossref_primary_10_1016_j_ymthe_2020_02_009
crossref_primary_10_1146_annurev_vision_082114_035532
crossref_primary_10_1016_j_ymthe_2020_11_029
crossref_primary_10_1038_mt_2015_121
crossref_primary_10_1002_adtp_202100164
crossref_primary_10_1038_gt_2017_63
crossref_primary_10_3389_fncel_2015_00494
crossref_primary_10_4155_fmc_2018_0315
crossref_primary_10_1073_pnas_2117038119
crossref_primary_10_1089_hum_2015_147
crossref_primary_10_1089_hum_2017_154
crossref_primary_10_1016_j_cobeha_2019_10_003
crossref_primary_10_1016_j_coph_2015_08_002
crossref_primary_10_3390_ijms222111542
crossref_primary_10_1016_j_preteyeres_2016_05_002
crossref_primary_10_1093_hmg_ddv341
crossref_primary_10_1016_j_isci_2023_107716
crossref_primary_10_1038_s44222_024_00210_4
crossref_primary_10_1089_hum_2016_111
crossref_primary_10_1051_medsci_2020095
crossref_primary_10_3389_fgeed_2021_737632
crossref_primary_10_1016_j_preteyeres_2022_101153
crossref_primary_10_1134_S0022093022060011
crossref_primary_10_1016_j_omtm_2020_05_009
Cites_doi 10.1126/science.1190897
10.1016/0092-8674(94)90151-1
10.1038/nn.3032
10.1038/nn.2370
10.1038/71021
10.3389/fmicb.2011.00204
10.1128/JVI.00218-12
10.1128/JVI.79.8.5047-5058.2005
10.1126/scitranslmed.3002103
10.1523/JNEUROSCI.0397-08.2008
10.1016/j.neuron.2006.02.026
10.1089/hum.2009.086
10.1523/JNEUROSCI.4044-13.2014
10.1038/mt.2009.277
10.1523/JNEUROSCI.17-09-03014.1997
10.2174/156652310793180706
10.1017/S0952523800012736
10.1167/iovs.05-0526
10.1152/jn.00137.2012
10.1128/JVI.00076-06
10.1073/pnas.1317033110
10.1002/cne.10703
10.1016/j.neuron.2012.10.002
10.1089/hum.2010.157
10.1038/nn.2776
10.1073/pnas.0908711106
10.1128/JVI.80.2.821-834.2006
10.1073/pnas.162250899
10.1056/NEJMoa0802315
10.1128/JVI.01304-07
10.1523/JNEUROSCI.18-21-08936.1998
10.1016/0092-8674(95)90354-2
10.1038/mt.2011.69
10.1007/978-1-61779-370-7_3
10.1038/nbt1182
10.1007/978-1-61779-370-7_8
10.1002/cne.21807
10.1128/JVI.77.20.11072-11081.2003
10.1038/nprot.2006.207
10.1089/hum.2005.16.408
10.1371/journal.pone.0023101
10.1093/nar/29.9.e45
10.1128/JVI.74.19.9281-9293.2000
10.1128/JVI.01288-06
10.1017/S0952523800006970
10.1093/nar/gnj003
10.1007/978-1-61737-982-6_8
10.1073/pnas.1936192100
10.1128/JVI.77.12.6995-7006.2003
10.1126/scitranslmed.3005708
10.1016/j.ajhg.2009.10.013
10.1038/nn.2117
10.1089/hum.2008.107
10.1038/nbt.1599
10.1056/NEJMoa0802268
ContentType Journal Article
Copyright The Authors. Published under the terms of the CC BY 4.0 license 2014
2014 The Authors. Published under the terms of the CC BY 4.0 license
2014 The Authors. Published under the terms of the CC BY 4.0 license.
2014. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Distributed under a Creative Commons Attribution 4.0 International License
2014 The Authors. Published under the terms of the CC BY 4.0 license 2014
Copyright_xml – notice: The Authors. Published under the terms of the CC BY 4.0 license 2014
– notice: 2014 The Authors. Published under the terms of the CC BY 4.0 license
– notice: 2014 The Authors. Published under the terms of the CC BY 4.0 license.
– notice: 2014. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: Distributed under a Creative Commons Attribution 4.0 International License
– notice: 2014 The Authors. Published under the terms of the CC BY 4.0 license 2014
DBID C6C
24P
AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7X7
7XB
8AO
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M7P
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
7X8
1XC
VOOES
5PM
ADTOC
UNPAY
DOA
DOI 10.15252/emmm.201404077
DatabaseName Springer Nature OA Free Journals
Wiley Online Library Open Access
CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
ProQuest Central (Corporate)
ProQuest Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
ProQuest Pharma Collection
ProQuest SciTech Collection
ProQuest Natural Science Journals
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
Health & Medical Collection (Alumni Edition)
Biological Science Database
ProQuest Central Premium
ProQuest One Academic
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
Hyper Article en Ligne (HAL)
Hyper Article en Ligne (HAL) (Open Access)
PubMed Central (Full Participant titles)
Unpaywall for CDI: Periodical Content
Unpaywall
DOAJ Open Access Full Text
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest Central Student
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Central China
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest Health & Medical Research Collection
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Central (New)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList MEDLINE


CrossRef
MEDLINE - Academic

Publicly Available Content Database
Database_xml – sequence: 1
  dbid: C6C
  name: Springer Nature OA Free Journals
  url: http://www.springeropen.com/
  sourceTypes: Publisher
– sequence: 2
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 3
  dbid: 24P
  name: Wiley Online Library Open Access
  url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html
  sourceTypes: Publisher
– sequence: 4
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 5
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 6
  dbid: UNPAY
  name: Unpaywall
  url: https://proxy.k.utb.cz/login?url=https://unpaywall.org/
  sourceTypes: Open Access Repository
– sequence: 7
  dbid: BENPR
  name: ProQuest Central
  url: http://www.proquest.com/pqcentral?accountid=15518
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Biology
EISSN 1757-4684
EndPage 1190
ExternalDocumentID oai_doaj_org_article_e4fc48eee17c4f51a495bf062825c437
10.15252/emmm.201404077
PMC4197864
oai:HAL:inserm-02894121v1
25092770
10_15252_emmm_201404077
EMMM201404077
Genre article
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: Swiss National Science Foundation
– fundername: NEI/NIH
  grantid: 8DP1 EY023177; 1R24EY019861‐01A1
– fundername: Research to prevent blindness
– fundername: FM Kirby Foundation
– fundername: Foundation Fighting Blindness
– fundername: Hope for Vision and the Marie Heim‐Vögtlin (MHV)
– fundername: NEI/NIH
  funderid: 8DP1 EY023177; 1R24EY019861‐01A1
– fundername: NCCDPHP CDC HHS
  grantid: 8DP1 EY023177
– fundername: NIH HHS
  grantid: DP1 OD008267
– fundername: NEI NIH HHS
  grantid: 1R24EY019861‐01A1
– fundername: NEI NIH HHS
  grantid: R24 EY019861
– fundername: NEI NIH HHS
  grantid: DP1 EY023177
– fundername: NEI NIH HHS
  grantid: P30 EY001583
GroupedDBID ---
0R~
1OC
24P
4.4
53G
5DZ
5GY
5VS
7X7
8-0
8-1
8AO
8FE
8FH
8FI
8FJ
AAHHS
AAJSJ
AAZKR
ABOCM
ABUWG
ACCFJ
ACCMX
ACGFO
ACGFS
ACPRK
ACXQS
ADBBV
ADKYN
ADPDF
ADRAZ
ADZMN
ADZOD
AEEZP
AEGXH
AENEX
AEQDE
AFBPY
AFKRA
AHMBA
AIAGR
AIWBW
AJBDE
ALAGY
ALIPV
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AOIJS
AVUZU
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BTFSW
BVXVI
C6C
CCPQU
D-9
DIK
DU5
EBD
EBS
EJD
EMOBN
F5P
FYUFA
GODZA
GROUPED_DOAJ
GX1
H13
HCIFZ
HK~
HMCUK
HYE
HZ~
IAO
IHR
ITC
KQ8
LH4
LK8
LW6
M48
M7P
M~E
NNB
O9-
OIG
OK1
OVD
OVEED
P2P
PIMPY
PQQKQ
PROAC
RHF
RHI
RNS
ROL
RPM
SV3
TEORI
UKHRP
WIN
XV2
31~
ABJNI
AFZJQ
EBLON
AAMMB
AASML
AAYXX
AEFGJ
AGXDD
AIDQK
AIDYY
AJAOE
CITATION
NAO
PHGZM
PHGZT
PQGLB
PUEGO
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7XB
8FK
AZQEC
DWQXO
GNUQQ
K9.
PJZUB
PKEHL
PPXIY
PQEST
PQUKI
PRINS
7X8
1XC
VOOES
5PM
ADTOC
AFFHD
UNPAY
ID FETCH-LOGICAL-c7527-d415d50aca2d52b79f719d03b0ad3e2cf295125e9ac0418aedd54da2eb38ab683
IEDL.DBID M48
ISSN 1757-4676
1757-4684
IngestDate Fri Oct 03 12:52:09 EDT 2025
Wed Oct 29 12:16:23 EDT 2025
Tue Sep 30 15:27:42 EDT 2025
Tue Oct 14 20:29:51 EDT 2025
Sun Sep 28 03:04:21 EDT 2025
Tue Oct 07 06:35:24 EDT 2025
Thu Apr 03 07:02:44 EDT 2025
Thu Apr 24 23:02:22 EDT 2025
Wed Oct 01 02:36:15 EDT 2025
Wed Jan 22 16:49:53 EST 2025
Fri Feb 21 02:36:45 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 9
Keywords capsid library
adeno‐associated virus
promoter optimization
multi‐electrode array
optogenetics
Neuroscience
adeno-associated virus
promoter optimization Subject Categories Genetics
Gene Therapy & Genetic Disease
multi-electrode array
Language English
License Attribution
2014 The Authors. Published under the terms of the CC BY 4.0 license.
Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0
This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
cc-by
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c7527-d415d50aca2d52b79f719d03b0ad3e2cf295125e9ac0418aedd54da2eb38ab683
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
PMCID: PMC4197864
Subject Categories Genetics, Gene Therapy & Genetic Disease; Neuroscience
ORCID 0000-0002-3508-4924
0000-0002-2938-2765
OpenAccessLink https://onlinelibrary.wiley.com/doi/abs/10.15252%2Femmm.201404077
PMID 25092770
PQID 2290127641
PQPubID 866378
PageCount 16
ParticipantIDs doaj_primary_oai_doaj_org_article_e4fc48eee17c4f51a495bf062825c437
unpaywall_primary_10_15252_emmm_201404077
pubmedcentral_primary_oai_pubmedcentral_nih_gov_4197864
hal_primary_oai_HAL_inserm_02894121v1
proquest_miscellaneous_1559618235
proquest_journals_2290127641
pubmed_primary_25092770
crossref_citationtrail_10_15252_emmm_201404077
crossref_primary_10_15252_emmm_201404077
wiley_primary_10_15252_emmm_201404077_EMMM201404077
springer_journals_10_15252_emmm_201404077
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate September 2014
PublicationDateYYYYMMDD 2014-09-01
PublicationDate_xml – month: 09
  year: 2014
  text: September 2014
PublicationDecade 2010
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
– name: Frankfurt
– name: Oxford, UK
PublicationTitle EMBO molecular medicine
PublicationTitleAbbrev EMBO Mol Med
PublicationTitleAlternate EMBO Mol Med
PublicationYear 2014
Publisher Nature Publishing Group UK
EMBO Press
Wiley Open Access
Blackwell Publishing Ltd
Springer Nature
Publisher_xml – name: Nature Publishing Group UK
– name: EMBO Press
– name: Wiley Open Access
– name: Blackwell Publishing Ltd
– name: Springer Nature
References Mitchell, Nicolson, Warischalk, Samulski (CR31) 2010; 10
Lochrie, Tatsuno, Christie, McDonnell, Zhou, Surosky, Pierce, Colosi (CR25) 2006; 80
Dhingra, Sulaiman, Xu, Fina, Veh, Vardi (CR10) 2008; 510
Doroudchi, Greenberg, Liu, Silka, Boyden, Lockridge, Arman, Janani, Boye, Boye (CR11) 2011; 19
Kim, Matsuda, Cepko (CR22) 2008; 28
Siegert, Scherf, Del Punta, Didkovsky, Heintz, Roska (CR100) 2009; 12
Vardi, Morigiwa (CR50) 1997; 14
Wu, Asokan, Grieger, Govindasamy, Agbandje‐McKenna, Samulski (CR102) 2006; 80
Shiells, Falk (CR44) 1994; 11
Bainbridge, Smith, Barker, Robbie, Henderson, Balaggan, Viswanathan, Holder, Stockman, Tyler (CR4) 2008; 358
Girod, Ried, Wobus, Lahm, Leike, Kleinschmidt, Deléage, Hallek (CR13) 1999; 5
Maguire, Simonelli, Pierce, Pugh, Mingozzi, Bennicelli, Banfi, Marshall, Testa, Surace (CR26) 2008; 358
Hanna, Calkins (CR16) 2007; 13
Kienle, Senís, Börner, Niopek, Wiedtke, Grosse, Grimm (CR21) 2012; pii
Nam, Lane, Padron, Gurda, McKenna, Kohlbrenner, Aslanidi, Byrne, Muzyczka, Zolotukhin (CR36) 2007; 81
Masland (CR28) 2012; 76
Vandenberghe, Bell, Maguire, Cearley, Xiao, Calcedo, Wang, Castle, Maguire, Grant (CR49) 2011; 3
Wobus, Hugle‐Dorr, Girod, Petersen, Hallek, Kleinschmidt (CR101) 2000; 74
Audo, Kohl, Leroy, Munier, Guillonneau, Mohand‐Saïd, Bujakowska, Nandrot, Lorenz, Preising (CR3) 2009; 85
Kern, Schmidt, Leder, Müller, Wobus, Bettinger, Von der Lieth, King, Kleinschmidt (CR20) 2003; 77
Agbandje‐McKenna, Kleinschmidt (CR1) 2011; 807
Ray, Heath, Hasan, Noel, Samuels, Martemyanov, Peachey, McCall, Gregg (CR43) 2014; 34
Jones, Watt, Frederick, Baehr, Chen, Levine, Milam, Lavail, Marc (CR19) 2003; 464
Nagel, Szellas, Huhn, Kateriya, Adeishvili, Berthold, Ollig, Hegemann, Bamberg (CR35) 2003; 100
Michelfelder, Varadi, Raupp, Hunger, Körbelin, Pahrmann, Schrepfer, Müller, Kleinschmidt, Trepel (CR30) 2011; 6
Kleinlogel, Feldbauer, Dempski, Fotis, Wood, Bamann, Bamberg (CR23) 2011; 14
Maheshri, Koerber, Kaspar, Schaffer (CR27) 2006; 24
Bi, Cui, Ma, Olshevskaya, Pu, Dizhoor, Pan (CR6) 2006; 50
Grieger, Choi, Samulski (CR14) 2006; 1
Padron, Bowman, Kaludov, Govindasamy, Levy, Nick, McKenna, Muzyczka, Chiorini, Baker (CR39) 2005; 79
Nomura, Shigemoto, Nakamura, Okamoto, Mizuno, Nakanishi (CR37) 1994; 77
Busskamp, Duebel, Balya, Fradot, Viney, Siegert, Groner, Cabuy, Forster, Seeliger (CR7) 2010; 329
Opie, Warrington, Agbandje‐McKenna, Zolotukhin, Muzyczka (CR38) 2003; 77
Perabo, Goldnau, White, Endell, Boucas, Humme, Work, Janicki, Hallek, Baker (CR41) 2006; 80
Yang, Li, Xiao (CR53) 2011; 709
Bartel, Schaffer, Büning (CR5) 2011; 2
Asokan, Conway, Phillips, Li, Hegge, Sinnott, Yadav, DiPrimio, Nam, Agbandje‐McKenna (CR2) 2010; 28
Xie, Bu, Bhatia, Hare, Somasundaram, Azzi, Chapman (CR52) 2002; 99
Jeon, Strettoi, Masland (CR18) 1998; 18
Ueda, Iwakabe, Masu, Suzuki, Nakanishi (CR48) 1997; 17
Stieger, Cronin, Bennett, Rolling (CR47) 2011; 807
Zeitz, van Genderen, Neidhardt, Luhmann, Hoeben, Forster, Wycisk, Mátyás, Hoyng, Riemslag (CR54) 2005; 46
Muzyczka, Warrington (CR34) 2005; 16
Muranaka, Hohsaka, Sisido (CR33) 2006; 34
Peachey, Pearring, Bojang, Hirschtritt, Sturgill‐Short, Ray, Furukawa, Koike, Goldberg, Shen (CR40) 2012; 108
Gurda, Raupp, Popa‐Wagner, Naumer, Olson, Ng, McKenna, Baker, Kleinschmidt, Agbandje‐McKenna (CR15) 2012; 86
Cideciyan, Hauswirth, Aleman, Kaushal, Schwartz, Boye, Windsor, Conlon, Sumaroka, Pang (CR8) 2009; 20
Simonelli, Maguire, Testa, Pierce, Mingozzi, Bennicelli, Rossi, Marshall, Banfi, Surace (CR46) 2010; 18
Pfaffl (CR42) 2001; 29
Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown (CR32) 2009; 106
Williams, Deisseroth (CR51) 2013; 110
Siegert, Cabuy, Scherf, Kohler, Panda, Le, Fehling, Gaidatzis, Stadler, Roska (CR45) 2012; 15
Hauswirth, Aleman, Kaushal, Cideciyan, Schwartz, Wang, Conlon, Boye, Flotte, Byrne (CR17) 2008; 19
Dalkara, Byrne, Klimczak, Visel, Yin, Merigan, Flannery, Schaffer (CR9) 2013; 5
Fradot, Busskamp, Forster, Cronin, Léveillard, Bennett, Sahel, Roska, Picaud (CR12) 2011; 22
Lagali, Balya, Awatramani, Münch, Kim, Busskamp, Cepko, Roska (CR24) 2008; 11
Masu, Iwakabe, Tagawa, Miyoshi, Yamashita, Fukuda, Sasaki, Hiroi, Nakamura, Shigemoto (CR29) 1995; 80
2010; 10
2009; 85
2006; 34
2010; 18
2002; 99
2011; 14
2012; 15
2013; 5
2011; 19
2009; 12
1998; 18
2006; 24
2010; 28
1997; 14
2011; 807
2008; 28
1994; 77
2011; 22
1997; 17
2008; 358
2008; 510
2013; 110
2005; 79
2011; 2
2006; 50
2010; 329
2009; 20
2008; 19
2008; 11
2006; 1
2001; 29
2011; 3
2011; 6
1999; 5
2007; 13
2012; 76
2012; 108
2003; 77
2005; 46
2006; 80
2012; pii
1995; 80
2000; 74
2011; 709
1994; 11
2007; 81
2005; 16
2003; 464
2003; 100
2014; 34
2012; 86
2009; 106
e_1_2_9_31_1
e_1_2_9_52_1
e_1_2_9_10_1
e_1_2_9_35_1
e_1_2_9_56_1
e_1_2_9_12_1
e_1_2_9_33_1
e_1_2_9_54_1
Ueda Y (e_1_2_9_50_1) 1997; 17
e_1_2_9_14_1
e_1_2_9_39_1
e_1_2_9_16_1
e_1_2_9_37_1
e_1_2_9_58_1
e_1_2_9_18_1
e_1_2_9_41_1
e_1_2_9_20_1
e_1_2_9_45_1
e_1_2_9_24_1
e_1_2_9_43_1
e_1_2_9_8_1
e_1_2_9_6_1
e_1_2_9_4_1
e_1_2_9_2_1
e_1_2_9_26_1
e_1_2_9_49_1
e_1_2_9_28_1
e_1_2_9_47_1
Hanna MC (e_1_2_9_17_1) 2007; 13
e_1_2_9_30_1
e_1_2_9_53_1
e_1_2_9_51_1
e_1_2_9_11_1
e_1_2_9_34_1
e_1_2_9_57_1
e_1_2_9_13_1
e_1_2_9_32_1
e_1_2_9_55_1
e_1_2_9_15_1
e_1_2_9_38_1
e_1_2_9_36_1
e_1_2_9_19_1
e_1_2_9_42_1
e_1_2_9_40_1
e_1_2_9_21_1
Kienle E (e_1_2_9_22_1) 2012
e_1_2_9_46_1
e_1_2_9_23_1
e_1_2_9_44_1
e_1_2_9_7_1
e_1_2_9_5_1
e_1_2_9_3_1
e_1_2_9_9_1
e_1_2_9_25_1
e_1_2_9_27_1
e_1_2_9_48_1
e_1_2_9_29_1
10581091 - Nat Med. 1999 Dec;5(12):1438
18671302 - J Comp Neurol. 2008 Oct 10;510(5):484-96
20576849 - Science. 2010 Jul 23;329(5990):413-7
9096137 - J Neurosci. 1997 May 1;17(9):3014-23
18774912 - Hum Gene Ther. 2008 Oct;19(10):979-90
11328886 - Nucleic Acids Res. 2001 May 1;29(9):e45
8181056 - Cell. 1994 May 6;77(3):361-9
22065962 - Front Microbiol. 2011 Oct 04;2:204
15871672 - Hum Gene Ther. 2005 Apr;16(4):408-16
10982375 - J Virol. 2000 Oct;74(19):9281-93
18441370 - N Engl J Med. 2008 May 22;358(21):2240-8
21850255 - PLoS One. 2011;6(8):e23101
19953081 - Mol Ther. 2010 Mar;18(3):643-50
12768018 - J Virol. 2003 Jun;77(12):6995-7006
12866125 - J Comp Neurol. 2003 Sep 8;464(1):1-16
19896113 - Am J Hum Genet. 2009 Nov;85(5):720-9
16249515 - Invest Ophthalmol Vis Sci. 2005 Nov;46(11):4328-35
19861548 - Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19174-8
22896717 - J Neurophysiol. 2012 Nov;108(9):2442-51
22034026 - Methods Mol Biol. 2011;807:47-92
14512555 - J Virol. 2003 Oct;77(20):11072-81
16397292 - Nucleic Acids Res. 2006;34(1):e7
21697530 - Sci Transl Med. 2011 Jun 22;3(88):88ra54
9786999 - J Neurosci. 1998 Nov 1;18(21):8936-46
20712583 - Curr Gene Ther. 2010 Oct;10(5):319-40
16378984 - J Virol. 2006 Jan;80(2):821-34
7841125 - Vis Neurosci. 1994 Nov-Dec;11(6):1175-83
22491297 - J Vis Exp. 2012;(62). pii: 3819. doi: 10.3791/3819
17728238 - J Virol. 2007 Nov;81(22):12260-71
12136130 - Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10405-10
16943302 - J Virol. 2006 Nov;80(22):11393-7
9279006 - Vis Neurosci. 1997 Jul-Aug;14(4):789-94
24790204 - J Neurosci. 2014 Apr 30;34(18):6334-43
16600853 - Neuron. 2006 Apr 6;50(1):23-33
23083731 - Neuron. 2012 Oct 18;76(2):266-80
22593150 - J Virol. 2012 Aug;86(15):7739-51
18441371 - N Engl J Med. 2008 May 22;358(21):2231-9
24106299 - Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16287
14615590 - Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5
23761039 - Sci Transl Med. 2013 Jun 12;5(189):189ra76
21142470 - Hum Gene Ther. 2011 May;22(5):587-93
18667607 - J Neurosci. 2008 Jul 30;28(31):7748-64
15795290 - J Virol. 2005 Apr;79(8):5047-58
16809332 - J Virol. 2006 Jul;80(14):7265-9
19648912 - Nat Neurosci. 2009 Sep;12(9):1197-204
7889569 - Cell. 1995 Mar 10;80(5):757-65
22034031 - Methods Mol Biol. 2011;807:179-218
18087239 - Mol Vis. 2007;13:2194-208
16429148 - Nat Biotechnol. 2006 Feb;24(2):198-204
20037580 - Nat Biotechnol. 2010 Jan;28(1):79-82
19583479 - Hum Gene Ther. 2009 Sep;20(9):999-1004
21505421 - Mol Ther. 2011 Jul;19(7):1220-9
21399632 - Nat Neurosci. 2011 Apr;14(4):513-8
18432197 - Nat Neurosci. 2008 Jun;11(6):667-75
21194025 - Methods Mol Biol. 2011;709:127-39
22267162 - Nat Neurosci. 2012 Mar;15(3):487-95, S1-2
17406430 - Nat Protoc. 2006;1(3):1412-28
References_xml – volume: 77
  start-page: 11072
  year: 2003
  end-page: 11081
  ident: CR20
  article-title: Identification of a heparin‐binding motif on adeno‐associated virus type 2 capsids
  publication-title: J Virol
– volume: 807
  start-page: 179
  year: 2011
  end-page: 218
  ident: CR47
  article-title: Adeno‐associated virus mediated gene therapy for retinal degenerative diseases
  publication-title: Methods Mol Biol
– volume: 6
  start-page: e23101
  year: 2011
  ident: CR30
  article-title: Peptide ligands incorporated into the threefold spike capsid domain to re‐direct gene transduction of AAV8 and AAV9
  publication-title: PLoS ONE
– volume: 16
  start-page: 408
  year: 2005
  end-page: 416
  ident: CR34
  article-title: Custom adeno‐associated virus capsids: the next generation of recombinant vectors with novel tropism
  publication-title: Hum Gene Ther
– volume: 29
  start-page: e45
  year: 2001
  ident: CR42
  article-title: A new mathematical model for relative quantification in real‐time RT‐PCR
  publication-title: Nucleic Acids Res
– volume: 34
  start-page: 6334
  year: 2014
  end-page: 6343
  ident: CR43
  article-title: GPR179 is required for high sensitivity of the mGluR6 signaling cascade in depolarizing bipolar cells
  publication-title: J Neurosci
– volume: 18
  start-page: 643
  year: 2010
  end-page: 650
  ident: CR46
  article-title: Gene therapy for Leber's congenital amaurosis is safe and effective through 1.5 years after vector administration
  publication-title: Mol Ther
– volume: 28
  start-page: 79
  year: 2010
  end-page: 82
  ident: CR2
  article-title: Reengineering a receptor footprint of adeno‐associated virus enables selective and systemic gene transfer to muscle
  publication-title: Nat Biotechnol
– volume: 5
  start-page: 1438
  year: 1999
  ident: CR13
  article-title: Genetic capsid modifications allow efficient re‐targeting of adeno‐associated virus type 2
  publication-title: Nat Med
– volume: 807
  start-page: 47
  year: 2011
  end-page: 92
  ident: CR1
  article-title: AAV capsid structure and cell interactions
  publication-title: Methods Mol Biol
– volume: 358
  start-page: 2231
  year: 2008
  end-page: 2239
  ident: CR4
  article-title: Effect of gene therapy on visual function in Leber's congenital amaurosis
  publication-title: N Engl J Med
– volume: 358
  start-page: 2240
  year: 2008
  end-page: 2248
  ident: CR26
  article-title: Safety and efficacy of gene transfer for Leber's congenital amaurosis
  publication-title: N Engl J Med
– volume: 13
  start-page: 2194
  year: 2007
  end-page: 2208
  ident: CR16
  article-title: Expression of genes encoding glutamate receptors and transporters in rod and cone bipolar cells of the primate retina determined by single‐cell polymerase chain reaction
  publication-title: Mol Vis
– volume: 1
  start-page: 1412
  year: 2006
  end-page: 1428
  ident: CR14
  article-title: Production and characterization of adeno‐associated viral vectors
  publication-title: Nat Protoc
– volume: 86
  start-page: 7739
  year: 2012
  end-page: 7751
  ident: CR15
  article-title: Mapping a neutralizing epitope onto the capsid of adeno‐associated virus serotype 8
  publication-title: J Virol
– volume: 99
  start-page: 10405
  year: 2002
  end-page: 10410
  ident: CR52
  article-title: The atomic structure of adeno‐associated virus (AAV‐2), a vector for human gene therapy
  publication-title: Proc Natl Acad Sci USA
– volume: 85
  start-page: 720
  year: 2009
  end-page: 729
  ident: CR3
  article-title: TRPM1 is mutated in patients with autosomal‐recessive complete congenital stationary night blindness
  publication-title: Am J Hum Genet
– volume: 81
  start-page: 12260
  year: 2007
  end-page: 12271
  ident: CR36
  article-title: Structure of adeno‐associated virus serotype 8, a gene therapy vector
  publication-title: J Virol
– volume: 18
  start-page: 8936
  year: 1998
  end-page: 8946
  ident: CR18
  article-title: The major cell populations of the mouse retina
  publication-title: J Neurosci
– volume: 24
  start-page: 198
  year: 2006
  end-page: 204
  ident: CR27
  article-title: Directed evolution of adeno‐associated virus yields enhanced gene delivery vectors
  publication-title: Nat Biotechnol
– volume: 20
  start-page: 999
  year: 2009
  end-page: 1004
  ident: CR8
  article-title: Human RPE65 gene therapy for Leber congenital amaurosis: persistence of early visual improvements and safety at 1 year
  publication-title: Hum Gene Ther
– volume: 11
  start-page: 1175
  year: 1994
  end-page: 1183
  ident: CR44
  article-title: Responses of rod bipolar cells isolated from dogfish retinal slices to concentration‐jumps of glutamate
  publication-title: Vis Neurosci
– volume: 12
  start-page: 1197
  year: 2009
  end-page: 1204
  ident: CR100
  article-title: Genetic address book for retinal cell types
  publication-title: Nat Neurosci
– volume: 22
  start-page: 587
  year: 2011
  end-page: 593
  ident: CR12
  article-title: Gene therapy in ophthalmology: validation on cultured retinal cells and explants from postmortem human eyes
  publication-title: Hum Gene Ther
– volume: 50
  start-page: 23
  year: 2006
  end-page: 33
  ident: CR6
  article-title: Ectopic expression of a microbial‐type rhodopsin restores visual responses in mice with photoreceptor degeneration
  publication-title: Neuron
– volume: 14
  start-page: 513
  year: 2011
  end-page: 518
  ident: CR23
  article-title: Ultra light‐sensitive and fast neuronal activation with the Ca ‐permeable channelrhodopsin CatCh
  publication-title: Nat Neurosci
– volume: 80
  start-page: 821
  year: 2006
  end-page: 834
  ident: CR25
  article-title: Mutations on the external surfaces of adeno‐associated virus type 2 capsids that affect transduction and neutralization
  publication-title: J Virol
– volume: 17
  start-page: 3014
  year: 1997
  end-page: 3023
  ident: CR48
  article-title: The mGluR6 5′ upstream transgene sequence directs a cell‐specific and developmentally regulated expression in retinal rod and ON‐type cone bipolar cells
  publication-title: J Neurosci
– volume: 100
  start-page: 13940
  year: 2003
  end-page: 13945
  ident: CR35
  article-title: Channelrhodopsin‐2, a directly light‐gated cation‐selective membrane channel
  publication-title: Proc Natl Acad Sci USA
– volume: 14
  start-page: 789
  year: 1997
  end-page: 794
  ident: CR50
  article-title: ON cone bipolar cells in rat express the metabotropic receptor mGluR6
  publication-title: Vis Neurosci
– volume: pii
  start-page: 3819
  year: 2012
  ident: CR21
  article-title: Engineering and evolution of synthetic adeno‐associated virus (AAV) gene therapy vectors via DNA family shuffling
  publication-title: J Vis Exp
– volume: 3
  start-page: 88ra54
  year: 2011
  ident: CR49
  article-title: Dosage thresholds for AAV2 and AAV8 photoreceptor gene therapy in monkey
  publication-title: Sci Transl Med
– volume: 80
  start-page: 7265
  year: 2006
  end-page: 7269
  ident: CR41
  article-title: Heparan sulfate proteoglycan binding properties of adeno‐associated virus retargeting mutants and consequences for their tropism
  publication-title: J Virol
– volume: 77
  start-page: 6995
  year: 2003
  end-page: 7006
  ident: CR38
  article-title: Identification of amino acid residues in the capsid proteins of adeno‐associated virus type 2 that contribute to heparan sulfate proteoglycan binding
  publication-title: J Virol
– volume: 10
  start-page: 319
  year: 2010
  end-page: 340
  ident: CR31
  article-title: AAV's anatomy: roadmap for optimizing vectors for translational success
  publication-title: Curr Gene Ther
– volume: 510
  start-page: 484
  year: 2008
  end-page: 496
  ident: CR10
  article-title: Probing neurochemical structure and function of retinal ON bipolar cells with a transgenic mouse
  publication-title: J Comp Neurol
– volume: 76
  start-page: 266
  year: 2012
  end-page: 280
  ident: CR28
  article-title: The neuronal organization of the retina
  publication-title: Neuron
– volume: 46
  start-page: 4328
  year: 2005
  end-page: 4335
  ident: CR54
  article-title: Mutations in GRM6 cause autosomal recessive congenital stationary night blindness with a distinctive scotopic 15‐Hz flicker electroretinogram
  publication-title: Invest Ophthalmol Vis Sci
– volume: 108
  start-page: 2442
  year: 2012
  end-page: 2451
  ident: CR40
  article-title: Depolarizing bipolar cell dysfunction due to a Trpm1 point mutation
  publication-title: J Neurophysiol
– volume: 19
  start-page: 1220
  year: 2011
  end-page: 1229
  ident: CR11
  article-title: Virally delivered channelrhodopsin‐2 safely and effectively restores visual function in multiple mouse models of blindness
  publication-title: Mol Ther
– volume: 80
  start-page: 757
  year: 1995
  end-page: 765
  ident: CR29
  article-title: Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene
  publication-title: Cell
– volume: 5
  start-page: 189ra76
  year: 2013
  ident: CR9
  article-title: ‐directed evolution of a new adeno‐associated virus for therapeutic outer retinal gene delivery from the vitreous
  publication-title: Sci Transl Med
– volume: 11
  start-page: 667
  year: 2008
  end-page: 675
  ident: CR24
  article-title: Light‐activated channels targeted to ON bipolar cells restore visual function in retinal degeneration
  publication-title: Nat Neurosci
– volume: 106
  start-page: 19174
  year: 2009
  end-page: 19178
  ident: CR32
  article-title: TRPM1 is required for the depolarizing light response in retinal ON‐bipolar cells
  publication-title: Proc Natl Acad Sci USA
– volume: 74
  start-page: 9281
  year: 2000
  end-page: 9293
  ident: CR101
  article-title: Monoclonal antibodies against the adeno‐associated virus type 2 (AAV‐2) capsid: epitope mapping and identification of capsid domains involved in AAV‐2‐cell interaction and neutralization of AAV‐2 infection
  publication-title: J Virol
– volume: 464
  start-page: 1
  year: 2003
  end-page: 16
  ident: CR19
  article-title: Retinal remodeling triggered by photoreceptor degenerations
  publication-title: J Comp Neurol
– volume: 2
  start-page: 204
  year: 2011
  ident: CR5
  article-title: Enhancing the clinical potential of AAV vectors by capsid engineering to evade pre‐existing immunity
  publication-title: Front Microbiol
– volume: 77
  start-page: 361
  year: 1994
  end-page: 369
  ident: CR37
  article-title: Developmentally regulated postsynaptic localization of a metabotropic glutamate receptor in rat rod bipolar cells
  publication-title: Cell
– volume: 709
  start-page: 127
  year: 2011
  end-page: 139
  ident: CR53
  article-title: Directed evolution of adeno‐associated virus (AAV) as vector for muscle gene therapy
  publication-title: Methods Mol Biol
– volume: 34
  start-page: e7
  year: 2006
  ident: CR33
  article-title: Four‐base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids
  publication-title: Nucleic Acids Res
– volume: 79
  start-page: 5047
  year: 2005
  end-page: 5058
  ident: CR39
  article-title: Structure of adeno‐associated virus type 4
  publication-title: J Virol
– volume: 80
  start-page: 11393
  year: 2006
  end-page: 11397
  ident: CR102
  article-title: Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno‐associated virus serotypes
  publication-title: J Virol
– volume: 19
  start-page: 979
  year: 2008
  end-page: 990
  ident: CR17
  article-title: Treatment of leber congenital amaurosis due to RPE65 mutations by ocular subretinal injection of adeno‐associated virus gene vector: short‐term results of a phase I trial
  publication-title: Hum Gene Ther
– volume: 28
  start-page: 7748
  year: 2008
  end-page: 7764
  ident: CR22
  article-title: A core paired‐type and POU homeodomain‐containing transcription factor program drives retinal bipolar cell gene expression
  publication-title: J Neurosci
– volume: 15
  start-page: 487
  year: 2012
  end-page: 495
  ident: CR45
  article-title: Transcriptional code and disease map for adult retinal cell types
  publication-title: Nat Neurosci
– volume: 329
  start-page: 413
  year: 2010
  end-page: 417
  ident: CR7
  article-title: Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa
  publication-title: Science
– volume: 110
  start-page: 16287
  year: 2013
  ident: CR51
  article-title: Optogenetics
  publication-title: Proc Natl Acad Sci USA
– volume: 29
  start-page: e45
  year: 2001
  article-title: A new mathematical model for relative quantification in real‐time RT‐PCR
  publication-title: Nucleic Acids Res
– volume: 28
  start-page: 7748
  year: 2008
  end-page: 7764
  article-title: A core paired‐type and POU homeodomain‐containing transcription factor program drives retinal bipolar cell gene expression
  publication-title: J Neurosci
– volume: 99
  start-page: 10405
  year: 2002
  end-page: 10410
  article-title: The atomic structure of adeno‐associated virus (AAV‐2), a vector for human gene therapy
  publication-title: Proc Natl Acad Sci USA
– volume: 19
  start-page: 979
  year: 2008
  end-page: 990
  article-title: Treatment of leber congenital amaurosis due to RPE65 mutations by ocular subretinal injection of adeno‐associated virus gene vector: short‐term results of a phase I trial
  publication-title: Hum Gene Ther
– volume: 16
  start-page: 408
  year: 2005
  end-page: 416
  article-title: Custom adeno‐associated virus capsids: the next generation of recombinant vectors with novel tropism
  publication-title: Hum Gene Ther
– volume: 358
  start-page: 2240
  year: 2008
  end-page: 2248
  article-title: Safety and efficacy of gene transfer for Leber's congenital amaurosis
  publication-title: N Engl J Med
– volume: 34
  start-page: e7
  year: 2006
  article-title: Four‐base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids
  publication-title: Nucleic Acids Res
– volume: 108
  start-page: 2442
  year: 2012
  end-page: 2451
  article-title: Depolarizing bipolar cell dysfunction due to a Trpm1 point mutation
  publication-title: J Neurophysiol
– volume: 14
  start-page: 513
  year: 2011
  end-page: 518
  article-title: Ultra light‐sensitive and fast neuronal activation with the Ca ‐permeable channelrhodopsin CatCh
  publication-title: Nat Neurosci
– volume: 24
  start-page: 198
  year: 2006
  end-page: 204
  article-title: Directed evolution of adeno‐associated virus yields enhanced gene delivery vectors
  publication-title: Nat Biotechnol
– volume: 106
  start-page: 19174
  year: 2009
  end-page: 19178
  article-title: TRPM1 is required for the depolarizing light response in retinal ON‐bipolar cells
  publication-title: Proc Natl Acad Sci USA
– volume: 50
  start-page: 23
  year: 2006
  end-page: 33
  article-title: Ectopic expression of a microbial‐type rhodopsin restores visual responses in mice with photoreceptor degeneration
  publication-title: Neuron
– volume: 79
  start-page: 5047
  year: 2005
  end-page: 5058
  article-title: Structure of adeno‐associated virus type 4
  publication-title: J Virol
– volume: 11
  start-page: 1175
  year: 1994
  end-page: 1183
  article-title: Responses of rod bipolar cells isolated from dogfish retinal slices to concentration‐jumps of glutamate
  publication-title: Vis Neurosci
– volume: 19
  start-page: 1220
  year: 2011
  end-page: 1229
  article-title: Virally delivered channelrhodopsin‐2 safely and effectively restores visual function in multiple mouse models of blindness
  publication-title: Mol Ther
– volume: 709
  start-page: 127
  year: 2011
  end-page: 139
  article-title: Directed evolution of adeno‐associated virus (AAV) as vector for muscle gene therapy
  publication-title: Methods Mol Biol
– volume: 17
  start-page: 3014
  year: 1997
  end-page: 3023
  article-title: The mGluR6 5′ upstream transgene sequence directs a cell‐specific and developmentally regulated expression in retinal rod and ON‐type cone bipolar cells
  publication-title: J Neurosci
– volume: 807
  start-page: 47
  year: 2011
  end-page: 92
  article-title: AAV capsid structure and cell interactions
  publication-title: Methods Mol Biol
– volume: 1
  start-page: 1412
  year: 2006
  end-page: 1428
  article-title: Production and characterization of adeno‐associated viral vectors
  publication-title: Nat Protoc
– volume: 2
  start-page: 204
  year: 2011
  article-title: Enhancing the clinical potential of AAV vectors by capsid engineering to evade pre‐existing immunity
  publication-title: Front Microbiol
– volume: 80
  start-page: 821
  year: 2006
  end-page: 834
  article-title: Mutations on the external surfaces of adeno‐associated virus type 2 capsids that affect transduction and neutralization
  publication-title: J Virol
– volume: 85
  start-page: 720
  year: 2009
  end-page: 729
  article-title: TRPM1 is mutated in patients with autosomal‐recessive complete congenital stationary night blindness
  publication-title: Am J Hum Genet
– volume: 10
  start-page: 319
  year: 2010
  end-page: 340
  article-title: AAV's anatomy: roadmap for optimizing vectors for translational success
  publication-title: Curr Gene Ther
– volume: 510
  start-page: 484
  year: 2008
  end-page: 496
  article-title: Probing neurochemical structure and function of retinal ON bipolar cells with a transgenic mouse
  publication-title: J Comp Neurol
– volume: 77
  start-page: 361
  year: 1994
  end-page: 369
  article-title: Developmentally regulated postsynaptic localization of a metabotropic glutamate receptor in rat rod bipolar cells
  publication-title: Cell
– volume: 100
  start-page: 13940
  year: 2003
  end-page: 13945
  article-title: Channelrhodopsin‐2, a directly light‐gated cation‐selective membrane channel
  publication-title: Proc Natl Acad Sci USA
– volume: 77
  start-page: 6995
  year: 2003
  end-page: 7006
  article-title: Identification of amino acid residues in the capsid proteins of adeno‐associated virus type 2 that contribute to heparan sulfate proteoglycan binding
  publication-title: J Virol
– volume: 110
  start-page: 16287
  year: 2013
  article-title: Optogenetics
  publication-title: Proc Natl Acad Sci USA
– volume: 6
  start-page: e23101
  year: 2011
  article-title: Peptide ligands incorporated into the threefold spike capsid domain to re‐direct gene transduction of AAV8 and AAV9
  publication-title: PLoS ONE
– volume: 12
  start-page: 1197
  year: 2009
  end-page: 1204
  article-title: Genetic address book for retinal cell types
  publication-title: Nat Neurosci
– volume: 807
  start-page: 179
  year: 2011
  end-page: 218
  article-title: Adeno‐associated virus mediated gene therapy for retinal degenerative diseases
  publication-title: Methods Mol Biol
– volume: 86
  start-page: 7739
  year: 2012
  end-page: 7751
  article-title: Mapping a neutralizing epitope onto the capsid of adeno‐associated virus serotype 8
  publication-title: J Virol
– volume: 46
  start-page: 4328
  year: 2005
  end-page: 4335
  article-title: Mutations in GRM6 cause autosomal recessive congenital stationary night blindness with a distinctive scotopic 15‐Hz flicker electroretinogram
  publication-title: Invest Ophthalmol Vis Sci
– volume: 5
  start-page: 1438
  year: 1999
  article-title: Genetic capsid modifications allow efficient re‐targeting of adeno‐associated virus type 2
  publication-title: Nat Med
– volume: 74
  start-page: 9281
  year: 2000
  end-page: 9293
  article-title: Monoclonal antibodies against the adeno‐associated virus type 2 (AAV‐2) capsid: epitope mapping and identification of capsid domains involved in AAV‐2‐cell interaction and neutralization of AAV‐2 infection
  publication-title: J Virol
– volume: 81
  start-page: 12260
  year: 2007
  end-page: 12271
  article-title: Structure of adeno‐associated virus serotype 8, a gene therapy vector
  publication-title: J Virol
– volume: 5
  start-page: 189ra76
  year: 2013
  article-title: ‐directed evolution of a new adeno‐associated virus for therapeutic outer retinal gene delivery from the vitreous
  publication-title: Sci Transl Med
– volume: 464
  start-page: 1
  year: 2003
  end-page: 16
  article-title: Retinal remodeling triggered by photoreceptor degenerations
  publication-title: J Comp Neurol
– volume: 34
  start-page: 6334
  year: 2014
  end-page: 6343
  article-title: GPR179 is required for high sensitivity of the mGluR6 signaling cascade in depolarizing bipolar cells
  publication-title: J Neurosci
– volume: 76
  start-page: 266
  year: 2012
  end-page: 280
  article-title: The neuronal organization of the retina
  publication-title: Neuron
– volume: 20
  start-page: 999
  year: 2009
  end-page: 1004
  article-title: Human RPE65 gene therapy for Leber congenital amaurosis: persistence of early visual improvements and safety at 1 year
  publication-title: Hum Gene Ther
– volume: 13
  start-page: 2194
  year: 2007
  end-page: 2208
  article-title: Expression of genes encoding glutamate receptors and transporters in rod and cone bipolar cells of the primate retina determined by single‐cell polymerase chain reaction
  publication-title: Mol Vis
– volume: 14
  start-page: 789
  year: 1997
  end-page: 794
  article-title: ON cone bipolar cells in rat express the metabotropic receptor mGluR6
  publication-title: Vis Neurosci
– volume: 15
  start-page: 487
  year: 2012
  end-page: 495
  article-title: Transcriptional code and disease map for adult retinal cell types
  publication-title: Nat Neurosci
– volume: 3
  start-page: 88ra54
  year: 2011
  article-title: Dosage thresholds for AAV2 and AAV8 photoreceptor gene therapy in monkey
  publication-title: Sci Transl Med
– volume: 18
  start-page: 643
  year: 2010
  end-page: 650
  article-title: Gene therapy for Leber's congenital amaurosis is safe and effective through 1.5 years after vector administration
  publication-title: Mol Ther
– volume: 329
  start-page: 413
  year: 2010
  end-page: 417
  article-title: Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa
  publication-title: Science
– volume: pii
  start-page: 3819
  year: 2012
  article-title: Engineering and evolution of synthetic adeno‐associated virus (AAV) gene therapy vectors via DNA family shuffling
  publication-title: J Vis Exp
– volume: 80
  start-page: 11393
  year: 2006
  end-page: 11397
  article-title: Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno‐associated virus serotypes
  publication-title: J Virol
– volume: 358
  start-page: 2231
  year: 2008
  end-page: 2239
  article-title: Effect of gene therapy on visual function in Leber's congenital amaurosis
  publication-title: N Engl J Med
– volume: 80
  start-page: 7265
  year: 2006
  end-page: 7269
  article-title: Heparan sulfate proteoglycan binding properties of adeno‐associated virus retargeting mutants and consequences for their tropism
  publication-title: J Virol
– volume: 22
  start-page: 587
  year: 2011
  end-page: 593
  article-title: Gene therapy in ophthalmology: validation on cultured retinal cells and explants from postmortem human eyes
  publication-title: Hum Gene Ther
– volume: 11
  start-page: 667
  year: 2008
  end-page: 675
  article-title: Light‐activated channels targeted to ON bipolar cells restore visual function in retinal degeneration
  publication-title: Nat Neurosci
– volume: 18
  start-page: 8936
  year: 1998
  end-page: 8946
  article-title: The major cell populations of the mouse retina
  publication-title: J Neurosci
– volume: 77
  start-page: 11072
  year: 2003
  end-page: 11081
  article-title: Identification of a heparin‐binding motif on adeno‐associated virus type 2 capsids
  publication-title: J Virol
– volume: 28
  start-page: 79
  year: 2010
  end-page: 82
  article-title: Reengineering a receptor footprint of adeno‐associated virus enables selective and systemic gene transfer to muscle
  publication-title: Nat Biotechnol
– volume: 80
  start-page: 757
  year: 1995
  end-page: 765
  article-title: Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene
  publication-title: Cell
– ident: e_1_2_9_8_1
  doi: 10.1126/science.1190897
– ident: e_1_2_9_38_1
  doi: 10.1016/0092-8674(94)90151-1
– ident: e_1_2_9_46_1
  doi: 10.1038/nn.3032
– ident: e_1_2_9_47_1
  doi: 10.1038/nn.2370
– ident: e_1_2_9_14_1
  doi: 10.1038/71021
– ident: e_1_2_9_6_1
  doi: 10.3389/fmicb.2011.00204
– ident: e_1_2_9_16_1
  doi: 10.1128/JVI.00218-12
– start-page: 3819
  year: 2012
  ident: e_1_2_9_22_1
  article-title: Engineering and evolution of synthetic adeno‐associated virus (AAV) gene therapy vectors via DNA family shuffling
  publication-title: J Vis Exp
– ident: e_1_2_9_40_1
  doi: 10.1128/JVI.79.8.5047-5058.2005
– volume: 13
  start-page: 2194
  year: 2007
  ident: e_1_2_9_17_1
  article-title: Expression of genes encoding glutamate receptors and transporters in rod and cone bipolar cells of the primate retina determined by single‐cell polymerase chain reaction
  publication-title: Mol Vis
– ident: e_1_2_9_51_1
  doi: 10.1126/scitranslmed.3002103
– ident: e_1_2_9_23_1
  doi: 10.1523/JNEUROSCI.0397-08.2008
– ident: e_1_2_9_7_1
  doi: 10.1016/j.neuron.2006.02.026
– ident: e_1_2_9_9_1
  doi: 10.1089/hum.2009.086
– ident: e_1_2_9_44_1
  doi: 10.1523/JNEUROSCI.4044-13.2014
– ident: e_1_2_9_48_1
  doi: 10.1038/mt.2009.277
– volume: 17
  start-page: 3014
  year: 1997
  ident: e_1_2_9_50_1
  article-title: The mGluR6 5′ upstream transgene sequence directs a cell‐specific and developmentally regulated expression in retinal rod and ON‐type cone bipolar cells
  publication-title: J Neurosci
  doi: 10.1523/JNEUROSCI.17-09-03014.1997
– ident: e_1_2_9_32_1
  doi: 10.2174/156652310793180706
– ident: e_1_2_9_52_1
  doi: 10.1017/S0952523800012736
– ident: e_1_2_9_58_1
  doi: 10.1167/iovs.05-0526
– ident: e_1_2_9_41_1
  doi: 10.1152/jn.00137.2012
– ident: e_1_2_9_42_1
  doi: 10.1128/JVI.00076-06
– ident: e_1_2_9_53_1
  doi: 10.1073/pnas.1317033110
– ident: e_1_2_9_20_1
  doi: 10.1002/cne.10703
– ident: e_1_2_9_29_1
  doi: 10.1016/j.neuron.2012.10.002
– ident: e_1_2_9_13_1
  doi: 10.1089/hum.2010.157
– ident: e_1_2_9_24_1
  doi: 10.1038/nn.2776
– ident: e_1_2_9_33_1
  doi: 10.1073/pnas.0908711106
– ident: e_1_2_9_26_1
  doi: 10.1128/JVI.80.2.821-834.2006
– ident: e_1_2_9_56_1
  doi: 10.1073/pnas.162250899
– ident: e_1_2_9_27_1
  doi: 10.1056/NEJMoa0802315
– ident: e_1_2_9_37_1
  doi: 10.1128/JVI.01304-07
– ident: e_1_2_9_19_1
  doi: 10.1523/JNEUROSCI.18-21-08936.1998
– ident: e_1_2_9_30_1
  doi: 10.1016/0092-8674(95)90354-2
– ident: e_1_2_9_12_1
  doi: 10.1038/mt.2011.69
– ident: e_1_2_9_2_1
  doi: 10.1007/978-1-61779-370-7_3
– ident: e_1_2_9_28_1
  doi: 10.1038/nbt1182
– ident: e_1_2_9_49_1
  doi: 10.1007/978-1-61779-370-7_8
– ident: e_1_2_9_11_1
  doi: 10.1002/cne.21807
– ident: e_1_2_9_21_1
  doi: 10.1128/JVI.77.20.11072-11081.2003
– ident: e_1_2_9_15_1
  doi: 10.1038/nprot.2006.207
– ident: e_1_2_9_35_1
  doi: 10.1089/hum.2005.16.408
– ident: e_1_2_9_31_1
  doi: 10.1371/journal.pone.0023101
– ident: e_1_2_9_43_1
  doi: 10.1093/nar/29.9.e45
– ident: e_1_2_9_54_1
  doi: 10.1128/JVI.74.19.9281-9293.2000
– ident: e_1_2_9_55_1
  doi: 10.1128/JVI.01288-06
– ident: e_1_2_9_45_1
  doi: 10.1017/S0952523800006970
– ident: e_1_2_9_34_1
  doi: 10.1093/nar/gnj003
– ident: e_1_2_9_57_1
  doi: 10.1007/978-1-61737-982-6_8
– ident: e_1_2_9_36_1
  doi: 10.1073/pnas.1936192100
– ident: e_1_2_9_39_1
  doi: 10.1128/JVI.77.12.6995-7006.2003
– ident: e_1_2_9_10_1
  doi: 10.1126/scitranslmed.3005708
– ident: e_1_2_9_4_1
  doi: 10.1016/j.ajhg.2009.10.013
– ident: e_1_2_9_25_1
  doi: 10.1038/nn.2117
– ident: e_1_2_9_18_1
  doi: 10.1089/hum.2008.107
– ident: e_1_2_9_3_1
  doi: 10.1038/nbt.1599
– ident: e_1_2_9_5_1
  doi: 10.1056/NEJMoa0802268
– reference: 12866125 - J Comp Neurol. 2003 Sep 8;464(1):1-16
– reference: 18774912 - Hum Gene Ther. 2008 Oct;19(10):979-90
– reference: 9096137 - J Neurosci. 1997 May 1;17(9):3014-23
– reference: 19648912 - Nat Neurosci. 2009 Sep;12(9):1197-204
– reference: 22267162 - Nat Neurosci. 2012 Mar;15(3):487-95, S1-2
– reference: 21697530 - Sci Transl Med. 2011 Jun 22;3(88):88ra54
– reference: 15795290 - J Virol. 2005 Apr;79(8):5047-58
– reference: 21505421 - Mol Ther. 2011 Jul;19(7):1220-9
– reference: 19953081 - Mol Ther. 2010 Mar;18(3):643-50
– reference: 10982375 - J Virol. 2000 Oct;74(19):9281-93
– reference: 19861548 - Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19174-8
– reference: 7841125 - Vis Neurosci. 1994 Nov-Dec;11(6):1175-83
– reference: 19583479 - Hum Gene Ther. 2009 Sep;20(9):999-1004
– reference: 20576849 - Science. 2010 Jul 23;329(5990):413-7
– reference: 18432197 - Nat Neurosci. 2008 Jun;11(6):667-75
– reference: 18087239 - Mol Vis. 2007;13:2194-208
– reference: 20037580 - Nat Biotechnol. 2010 Jan;28(1):79-82
– reference: 12768018 - J Virol. 2003 Jun;77(12):6995-7006
– reference: 22034026 - Methods Mol Biol. 2011;807:47-92
– reference: 22034031 - Methods Mol Biol. 2011;807:179-218
– reference: 22593150 - J Virol. 2012 Aug;86(15):7739-51
– reference: 7889569 - Cell. 1995 Mar 10;80(5):757-65
– reference: 22065962 - Front Microbiol. 2011 Oct 04;2:204
– reference: 20712583 - Curr Gene Ther. 2010 Oct;10(5):319-40
– reference: 19896113 - Am J Hum Genet. 2009 Nov;85(5):720-9
– reference: 21399632 - Nat Neurosci. 2011 Apr;14(4):513-8
– reference: 18671302 - J Comp Neurol. 2008 Oct 10;510(5):484-96
– reference: 15871672 - Hum Gene Ther. 2005 Apr;16(4):408-16
– reference: 22896717 - J Neurophysiol. 2012 Nov;108(9):2442-51
– reference: 21142470 - Hum Gene Ther. 2011 May;22(5):587-93
– reference: 9279006 - Vis Neurosci. 1997 Jul-Aug;14(4):789-94
– reference: 16600853 - Neuron. 2006 Apr 6;50(1):23-33
– reference: 22491297 - J Vis Exp. 2012;(62). pii: 3819. doi: 10.3791/3819
– reference: 14615590 - Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5
– reference: 10581091 - Nat Med. 1999 Dec;5(12):1438
– reference: 18441370 - N Engl J Med. 2008 May 22;358(21):2240-8
– reference: 14512555 - J Virol. 2003 Oct;77(20):11072-81
– reference: 21194025 - Methods Mol Biol. 2011;709:127-39
– reference: 16943302 - J Virol. 2006 Nov;80(22):11393-7
– reference: 17406430 - Nat Protoc. 2006;1(3):1412-28
– reference: 16397292 - Nucleic Acids Res. 2006;34(1):e7
– reference: 12136130 - Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10405-10
– reference: 18667607 - J Neurosci. 2008 Jul 30;28(31):7748-64
– reference: 16809332 - J Virol. 2006 Jul;80(14):7265-9
– reference: 18441371 - N Engl J Med. 2008 May 22;358(21):2231-9
– reference: 24106299 - Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16287
– reference: 23761039 - Sci Transl Med. 2013 Jun 12;5(189):189ra76
– reference: 16378984 - J Virol. 2006 Jan;80(2):821-34
– reference: 9786999 - J Neurosci. 1998 Nov 1;18(21):8936-46
– reference: 8181056 - Cell. 1994 May 6;77(3):361-9
– reference: 16429148 - Nat Biotechnol. 2006 Feb;24(2):198-204
– reference: 21850255 - PLoS One. 2011;6(8):e23101
– reference: 16249515 - Invest Ophthalmol Vis Sci. 2005 Nov;46(11):4328-35
– reference: 17728238 - J Virol. 2007 Nov;81(22):12260-71
– reference: 23083731 - Neuron. 2012 Oct 18;76(2):266-80
– reference: 11328886 - Nucleic Acids Res. 2001 May 1;29(9):e45
– reference: 24790204 - J Neurosci. 2014 Apr 30;34(18):6334-43
SSID ssj0065618
Score 2.4664013
Snippet In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar cells. The...
In this report, we describe the development of a modified adeno‐associated virus ( AAV ) capsid and promoter for transduction of retinal ON ‐bipolar cells. The...
In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The...
Abstract In this report, we describe the development of a modified adeno‐associated virus (AAV) capsid and promoter for transduction of retinal ON‐bipolar...
SourceID doaj
unpaywall
pubmedcentral
hal
proquest
pubmed
crossref
wiley
springer
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 1175
SubjectTerms adeno‐associated virus
Animals
Binding sites
Bioengineering
Bipolar cells
Blindness
capsid library
Clinical trials
Dependovirus - genetics
EMBO16
EMBO27
Experiments
Ganglion cells
Gene expression
Gene therapy
Genetic Vectors
HEK293 Cells
Heparan sulfate
Humans
Imaging
Libraries
Life Sciences
Light
Mice
Mice, Inbred C57BL
multi‐electrode array
Mutagenesis
Mutagenesis, Site-Directed
Neurobiology
Neurons and Cognition
optogenetics
Photoreceptors
promoter optimization
Promoter Regions, Genetic
Research Article
Retina
Retinal Bipolar Cells - virology
Transduction, Genetic - methods
Vectors (Biology)
SummonAdditionalLinks – databaseName: DOAJ Open Access Full Text
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQJV4HBOUVKMhIINFDaOLYcXIsaKsVYjlRqTfLsR11pd0k2uwW7Y0bV34jv4QZJxuIqmovHBM7seP5PI_Y85mQt-DWG2aFDfO8lCGXLgmL0uVhluU8NXnJrfVsn1_T6Tn_fCEu_jnqC_eEdfTA3cCdOF4anjnnYml4KWINHn1RYuYfE4YnPo88yvJdMNXpYHBS_J89sI3QgVSmPamPYIKduOUSU9CRVyaScmSPPG0_WJlL3BR53eO8vnFyWD29T-5uqkZvv-vFYuznekN19pA86D1Metp92SNyy1WH5HZ35uT2kNyZ9avpj8nPieePgFboGk2W7Zhkqa4srZt1DdjCFEcKWmDZn_JF65Ji3iO2UMwbjIsp_vtvabGlmrbbChxKfEaDPqt___ile_E7S6_mq01LjW5gAvg2Gr8V0K2ekPOzybdP07A_mCE0UjAZWrD6VkTaaBAzKySIOc5tlBSRtoljpmTgtzHhcm0iHmfaWSu41QwC90wXaZY8JQdVXbnnhGaCO8cSC4GV5dqVudAQwZSF1ZnRBdMB-bATjzI9azkenrFQGL2gPBXKUw3yDMj74YGmI-y4uepHlPdQDZm2_Q3An-rxp_bhLyDvAC2jd0xPv6h5BRpkqXAdl8csvooDcrTDk-rVRKuQbD9mMuVQ_GYohgmOktOVqzetwnVjQDNLRECedfAbWgP_NWdSRgGRI2COujMuqeaXnkScx7nMUh6Q4x2E_3brxvE6HjC-f2wTPwf21VOT2Ww2XL34HxJ5Se7hC7uNgEfkYL3auFfgOa6L115J_AFrSWoc
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV1Lb9QwELbKVrwOCMorUJCRQKKH0I1jx8kBoRZttUJshRCVeosc26Er7SZhH0V748aV38gvYcZ5VFFVekzixJbn83gmM_6GkNdg1mtmhPGTJJc-lzb0s9wmfhwnPNJJzo1xbJ_H0fiEfzoVp1vkuD0Lg2mVrU50itqUGv-R7yMvecBkxIMP1Q8fq0ZhdLUtoaGa0grmvaMYu0G2GTJjDcj24ej4y9dWN4Px4v74wZ4JA4tk1JD9CCbYvp3P8Wg68s0MpeztU47OH3afM0yWvGyJXk6o7KKqd8ntdVGpzU81m_XtX7eBHd0n9xrLkx7UUHlAtmyxQ27WtSg3O-TWpImyPyS_R45XAnqhK9zKTM0wS1VhaFmtSsAcHn2koB3mTfUvWuYUz0NiD9m0Qn-ZYkxgSbMNVXS5KcDQxHcU6Lny768_qoGFNfR8ulgvqVYVLAzXR-VSBO3iETk5Gn37OPabgg2-loJJ34A1YMRQaQXiZ5kE8QeJGYbZUJnQMp0zsOeYsInSQx7EyhojuFEMHPpYZVEcPiaDoizsU0Jjwa1loQGHy3Bl80Qo8GzyzKhYq4wpj7xrxZPqhs0ci2rMUvRqUJ4pyjPt5OmRt90LVU3kcXXTQ5R31wwZuN2NcvE9bRZ0anmueWytDaTmuQgUeJpZjidSmdA8hI-8AbT0vjE--JxOC9As8xTjuxwQeh54ZLfFU9qoj2V6AXaPvOoew8JHyanClutlivFkQDMLhUee1PDregO7NmFSDj0ie8DsDaf_pJieOXJxHiQyjrhH9loIXwzryvna6zB-_dyGbg1c1y4dTSaT7urZ_yfpObmDTevUv10yWC3W9gXYiqvsZaMA_gGcAGfj
  priority: 102
  providerName: ProQuest
– databaseName: Springer Nature OA Free Journals
  dbid: C6C
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELagiNcBQYESKMhIINFDSuLYcXIs1VYrxHKiUm-WYzvqSrtJtI9We-PGld_IL2HGyQaiUlXcktjxWJnPnpmM_ZmQd-DWG2aFDfO8lCGXLgmL0uVhluU8NXnJrfVsn1_T8Sn_fCbOOpIk3Avzd_5eMME-uvkcN4wjC0wk5W1yByxU6rOy6fF2ygWfxP_IA1MI8lKZdhw-_2hgYH48Sz8YlXNcA3nVwby6TrJPlj4k99dVozeXejYburXeLp08Jo86h5IetQh4Qm65apfcbY-Y3OySe5Muef6U_Bh5ugiQQldooWxLHEt1ZWndrGqAEu5opDDo592hXrQuKW5zRAnFtMEwmOKv_iUtNlTT5aYC_xHf0TB91b--_9Sdtp2lF9PFekmNbgDvXkbjV_65xTNyejL6djwOu3MYQiMFk6EFI29FpI0GrbJCglbj3EZJEWmbOGZKBm4aEy7XJuJxpp21glvNIE7PdJFmyXOyU9WVe0FoJrhzLLEQR1muXZkLDQFLWVidGV0wHZDDrXqU6UjK8ayMmcJgBfWpUJ-q12dAPvQvNC0_x_VVP6G--2pIrO0fAN5UN06V46XhmXMuloaXItYQQBYlbjRlwvAEGnkPaBm0MT76oqYVTBhzhWlbHrP4Ig7I_hZPqpsVlgq59WMmUw7Fb_tiGM-oOV25er1UmCYGNLNEBGSvhV8vDdzVnEkZBUQOgDnozrCkmp57znAe5zJLeUAOthD-061rv9dBj_Gbv23ix8BN9dRoMpn0dy__ozevyAO8bpf37ZOd1WLtXoM_uCre-LngN4BqWe4
  priority: 102
  providerName: Springer Nature
– databaseName: Unpaywall
  dbid: UNPAY
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lj9MwELaWrngdeOzyCCzISCCxh5TGsePkWFBXFaIrDlRaTpFjO2xFm0R9LConblz5jfwSZpw0qCyrFeKWxo7tTmbGM_HMN4Q8B7NeMyOMnyS59Lm0oZ_lNvHjOOGRTnJujEP7PI6GY_72RJzskMEmF6bGh2g_uKFkOH2NAl6ZvNbzm7o97JWdzTCdHDFielJeIbuRAJO8Q3bHx-_7H10ypIAlRK7IXHMd8wbi5y8jbO1ODsQf9pxTDJE8b3-eD6Nsz1JvkuurolLrL2o63bZ63bZ1dLsOL1k4tEOMVvncXS2zrv76Bxbkf1PkDrnVGLa0X3PiXbJjiz1ytS51ud4j10bNIf4--T5wsBXwd-gSd0pTA9hSVRhaVssSWBozKykon1lTXIyWOcV0S5whm1TojlM8cljQbE0VXawLsGPxGQVqtPz57YdquM4aejaZrxZUqwrkzs1RuQhEO79HxkeDD2-GflMPwtdSMOkbMDaM6CmtgLtYJoG7gsT0wqynTGiZzhmYi0zYROkeD2JljRHcKGazMFZZFIf3SacoC_uQ0Fhwa1lowJ8zXNk8EQocpzwzKtYqY8oj3Q0fpLoBS8eaHdMUnSYkdIqETltCe-Rl-0BV44Rc3PU1MlbbDQG-3Y1y_ilt9EVqea55bK0NpOa5CBQ4slmOCa9MaB7CIC-ALbfGGPbfpZMCFNcsxeNjHrDgLPDIwYZx00Y7LVLE-A-YjDg0P2ubQa_gm1OFLVeLFI-rI3A-Q-GRBzWft7OB2ZwwKXsekVsSsLWc7ZZicuqwy3mQyDjiHjncyMrvZV1Ir8NWmC6nbegk5LJ-6WA0GrW_Hv3DDI_JDbyuwwwPSGc5X9knYJcus6eN1vkFm2CKXA
  priority: 102
  providerName: Unpaywall
– databaseName: Wiley Online Library Open Access
  dbid: 24P
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Li9QwGA-64usgur6qq0RQcA_VaZo07XGVWQZxxIML3kKapLsDM22ZzqzMzZtX_0b_Er8vfUhZlgVv08mTfr_v1SS_EPIawnrDrLBhlhUy5NLFYV64LEzTjCcmK7i1nu3zSzI74Z--i343IZ6Fafkhhg9uqBneXqOC67zpb-xB1lC3WuFRcuSHmUh5ndyIIJpBkDP-tTfGEK34T3zgJGEmiUw6dh_s4v24g5Fj8vz94G7OcHfkxdDz4g7KYRn1Lrm9LWu9-6GXy3HA6z3W8X1yrws16VGLjQfkmiv3yc328sndPrk175bVH5JfU08kAaPQDfou21LKUl1aWtWbCkCGZx0pmINVd90XrQqKByBxhHxRY4JMcRGgofmOatrsSogssY0Gw1b9-flbdzhwlp4v1tuGGl2DJvgxar8n0K0fkZPj6bePs7C7oSE0UjAZWnD_Vky00SBvlkuQd5TZSZxPtI0dMwWDAI4Jl2kz4VGqnbWCW80gg091nqTxY7JXVqV7SmgquHMstpBhWa5dkQkNqUyRW50anTMdkHe9eJTp6MvxFo2lwjQG5alQnmqQZ0DeDg3qlrnj8qofUN5DNaTc9n9U61PVabByvDA8dc5F0vBCRBpSy7zAI6hMGB5DJ28ALaM-Zkef1aIEU7JSuKDLIxadRwE56PGkOnvRKGTdj5hMOBS_GopB01FyunTVtlG4gAxoZrEIyJMWfsNoEMhmTMpJQOQImKPpjEvKxZlnE-dRJtOEB-Swh_C_aV36vg4HjF_9bmOvA1fVU9P5fD48PfuvVs_JHfzdbgE8IHub9da9gJhxk7_0VuEvP4plfg
  priority: 102
  providerName: Wiley-Blackwell
Title Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno‐associated virus capsid and promoter
URI https://link.springer.com/article/10.15252/emmm.201404077
https://onlinelibrary.wiley.com/doi/abs/10.15252%2Femmm.201404077
https://www.ncbi.nlm.nih.gov/pubmed/25092770
https://www.proquest.com/docview/2290127641
https://www.proquest.com/docview/1559618235
https://inserm.hal.science/inserm-02894121
https://pubmed.ncbi.nlm.nih.gov/PMC4197864
https://onlinelibrary.wiley.com/doi/pdfdirect/10.15252/emmm.201404077
https://doaj.org/article/e4fc48eee17c4f51a495bf062825c437
UnpaywallVersion publishedVersion
Volume 6
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAFT
  databaseName: Open Access Digital Library
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: KQ8
  dateStart: 20090101
  isFulltext: true
  titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html
  providerName: Colorado Alliance of Research Libraries
– providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: DOA
  dateStart: 20090101
  isFulltext: true
  titleUrlDefault: https://www.doaj.org/
  providerName: Directory of Open Access Journals
– providerCode: PRVBFR
  databaseName: Free Medical Journals
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: DIK
  dateStart: 20090101
  isFulltext: true
  titleUrlDefault: http://www.freemedicaljournals.com
  providerName: Flying Publisher
– providerCode: PRVFQY
  databaseName: GFMER Free Medical Journals
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: GX1
  dateStart: 0
  isFulltext: true
  titleUrlDefault: http://www.gfmer.ch/Medical_journals/Free_medical.php
  providerName: Geneva Foundation for Medical Education and Research
– providerCode: PRVAQN
  databaseName: PubMed Central
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: RPM
  dateStart: 20090101
  isFulltext: true
  titleUrlDefault: https://www.ncbi.nlm.nih.gov/pmc/
  providerName: National Library of Medicine
– providerCode: PRVPQU
  databaseName: Health & Medical Collection
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: 7X7
  dateStart: 20090401
  isFulltext: true
  titleUrlDefault: https://search.proquest.com/healthcomplete
  providerName: ProQuest
– providerCode: PRVPQU
  databaseName: ProQuest Central
  customDbUrl: http://www.proquest.com/pqcentral?accountid=15518
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: BENPR
  dateStart: 20090401
  isFulltext: true
  titleUrlDefault: https://www.proquest.com/central
  providerName: ProQuest
– providerCode: PRVFZP
  databaseName: Scholars Portal Journals: Open Access
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 20250731
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: M48
  dateStart: 20090401
  isFulltext: true
  titleUrlDefault: http://journals.scholarsportal.info
  providerName: Scholars Portal
– providerCode: PRVAVX
  databaseName: Springer Nature HAS Fully OA
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: AAJSJ
  dateStart: 20090401
  isFulltext: true
  titleUrlDefault: https://www.springernature.com
  providerName: Springer Nature
– providerCode: PRVWIB
  databaseName: KBPluse Wiley Online Library: Open Access
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 99991231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: AVUZU
  dateStart: 20090101
  isFulltext: true
  titleUrlDefault: https://www.kbplus.ac.uk/kbplus7/publicExport/pkg/559
  providerName: Wiley-Blackwell
– providerCode: PRVWIB
  databaseName: Wiley Online Library Open Access
  customDbUrl:
  eissn: 1757-4684
  dateEnd: 20231231
  omitProxy: true
  ssIdentifier: ssj0065618
  issn: 1757-4676
  databaseCode: 24P
  dateStart: 20090101
  isFulltext: true
  titleUrlDefault: https://authorservices.wiley.com/open-science/open-access/browse-journals.html
  providerName: Wiley-Blackwell
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3Nb9MwFLfYJr4OCMaAwKiMBBI7ZCSOEycHhLqqU4VoVSEqlZPl2A6r1CahH4PeuHHlb-Qv4dlJM1XbNC5VEye25fez33t59u8h9BrMeklUqNwkyZhLmQ7cNNOJG8cJjWSSUaUs2-cg6o3ox3E4vkgHVA_g4krXzuSTGs2nxz-_rz_AhH9fZ-8h7_RsZg6VG6YYj7EdtAdqKjF5HPq0CSmA3WI_9oG6hD5FLKp5fq6oYEtFWSZ_UDxnZp_kZSP08l7KJqB6H91d5aVY_xDT6bbpa3XX6UP0oDY6cbtCySN0S-f76HaVhnK9j-706wD7Y_S7aykloBW8NFpMVeSyWOQKF-WyALiZU48YFoZZnfgLFxk2RyFNC-mkNOOJTThggdM1FnixzsHGNO8IWOKKv7_-iBoRWuHzyXy1wFKUMCdsG6XdHajnB2h02v3S6bl1rgZXspAwV4EhoEJPSAGSJykDyfuJ8oLUEyrQRGYETDkS6kRIj_qx0EqFVAkCvnws0igOnqDdvMj1M4TjkGpNAgW-lqJCZ0kowKnJUiViKVIiHHS8EQ-XNZG5yacx5cahMfLkRp68kaeD3jYvlBWHx_WPnhh5N48Z8m17o5h_4_Vc5ppmksZaa59JmoW-ACczzcxhVBJKGkAlbwAtW3X02p_4JIdFZcZNaJf6xD_3HXS4wRPfAJ8b_n2fsIhC8aumGOa8kZzIdbFacBNKBjSTIHTQ0wp-TWtg0iaEMc9BbAuYW93ZLsknZ5ZXnPoJiyPqoKMNhC-6de14HTUYv3lsAzsHbnqOd_v9fnP1_P-F9wLdM_-rHYCHaHc5X-mXYDIu0xbaIXQIv2zMWmjvpDsYfoarTtRp2Y8wLbtUQMloMGx__QeNKG7l
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELZKKygcEJRXoICRqEQPoRvHjpNDhVrYakt3K4Raqbfg2A5daTcJ-2i1N25c-UX8GH4JM9kk1aoqPfWYxC95xvPIeL4h5C2Y9ZoZYdwoSqXLpfXdJLWRG4YRD3SUcmNKtM_DoHPMP5-IkyXyp86FwWuVtUwsBbXJNf4j30Jcco_JgHsfih8uVo3C6GpdQkNVpRXMdgkxViV2HNjZObhw4-39T0DvDcb22kcfO25VZcDVUjDpGlBhRrSUVrBmlkhYsxeZlp-0lPEt0ykDI4QJGynd4l6orDGCG8XACw1VEoQ-jHuLrHCfR-D8rey2D798rXUBGEvlH0bQ0bARgQwqcCHBBNuywyGmwiO-TUvKBb1Ylg8AbXeKlzMvW76XL3A2Udx7ZHWaFWp2rgaDRXu7VJh7D8j9ytKlO3PWfEiWbLZGbs9rX87WyJ1eFdV_RH61SxwLmIVOUHWaOaItVZmheTHJgccx1ZKCNBpW1cZonlLMv8QZkn6B_jnFGMSYJjOq6HiWgWGLfRTI1fzvz9-qYkNr6Fl_NB1TrQo4iOUcRXkl0Y4ek-MbId0TspzlmX1GaCi4tcw34OAZrmwaCQWeVJoYFWqVMOWQ9zV5Yl2hp2MRj0GMXhTSM0Z6xg09HfKu6VDMgUOubrqL9G6aIeJ3-SIffY8rARJbnmoeWms9qXkqPAWebZJiBiwTmvswyAZwy8IYnZ1u3M9Akg1jjCdzj3lnnkPWa36KK3E1ji8Ol0PeNJ9B0CDlVGbz6TjG-DVwM_OFQ57O2a-ZDezoiEnZcohcYMyF5Sx-yfqnJZg59yIZBtwhmzULXyzryv3abHj8-r31yzNwXbu43ev1mqfn_9-k12S1c9Trxt39w4MX5C52m187XCfLk9HUvgQ7dZK8qoQBJd9uWv78AxgupXc
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELZKEQUOCMorUMBIVKKH0I1jx8kBoUK3amm34kCl3oxjO3Sl3STso9XeuHHl9_Bz-CXMJNlUq6r01GMSv-QZfzOTGc8Q8gbUesOssH6SZNLn0oV-mrnEj-OERybJuLVVts_DaPeIfz4Wx0vkz_wuDIZVzjGxAmpbGPxHvol5yQMmIx5sZk1YxJftnQ_lDx8rSKGndV5Oo2aRfTc7A_Nt_H5vG2i9zthO9-unXb-pMOAbKZj0LYgvKzraaFgvSyWsN0hsJ0w72oaOmYyBAsKES7Tp8CDWzlrBrWZggcY6jeIQxr1BbsowTDCcUB63xh6oSdW_RZDOsAWRjJq0QoIJtumGQ7wEj5ltOlIuSMSqcADIuRMMy7yo814M3Wz9t3fJ7Wle6tmZHgwWNe1KVO7cJ_caHZdu1Uz5gCy5fJXcqqtezlbJSq_x5z8kv7pVBguYhU5QaNo6ly3VuaVFOSmAu_GSJQUcGjZ1xmiRUbx5iTOk_RItc4rehzFNZ1TT8SwHlRb7aEDU4u_P37phQGfpaX80HVOjSziC1RxlFYzoRo_I0bUQ7jFZzovcPSU0Ftw5Flow7SzXLkuEBhsqS62OjU6Z9si7OXmUafKmY_mOgUL7CempkJ6qpadH3rYdyjplyOVNPyK922aY67t6UYy-qwY6lOOZ4bFzLpCGZyLQYNOmGd59ZcLwEAZZB25ZGGN360D1c8CwoUJPMg9YcBp4ZG3OT6oBqrE6P1Yeed1-BohByuncFdOxQs81cDMLhUee1OzXzgYadMKk7HhELjDmwnIWv-T9kyqNOQ8SGUfcIxtzFj5f1qX7tdHy-NV7G1Zn4Kp2qtvr9dqnZ__fpFdkBVBHHewd7j8nd7BXHW-4RpYno6l7AQrqJH1ZIQEl364bev4BjVCjEQ
linkToUnpaywall http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lj9MwELaWrngdeOzyCCzISCCxh5TGsePkWFBXFaIrDlRaTpFjO2xFm0R9LConblz5jfwSZpw0qCyrFeKWxo7tTmbGM_HMN4Q8B7NeMyOMnyS59Lm0oZ_lNvHjOOGRTnJujEP7PI6GY_72RJzskMEmF6bGh2g_uKFkOH2NAl6ZvNbzm7o97JWdzTCdHDFielJeIbuRAJO8Q3bHx-_7H10ypIAlRK7IXHMd8wbi5y8jbO1ODsQf9pxTDJE8b3-eD6Nsz1JvkuurolLrL2o63bZ63bZ1dLsOL1k4tEOMVvncXS2zrv76Bxbkf1PkDrnVGLa0X3PiXbJjiz1ytS51ud4j10bNIf4--T5wsBXwd-gSd0pTA9hSVRhaVssSWBozKykon1lTXIyWOcV0S5whm1TojlM8cljQbE0VXawLsGPxGQVqtPz57YdquM4aejaZrxZUqwrkzs1RuQhEO79HxkeDD2-GflMPwtdSMOkbMDaM6CmtgLtYJoG7gsT0wqynTGiZzhmYi0zYROkeD2JljRHcKGazMFZZFIf3SacoC_uQ0Fhwa1lowJ8zXNk8EQocpzwzKtYqY8oj3Q0fpLoBS8eaHdMUnSYkdIqETltCe-Rl-0BV44Rc3PU1MlbbDQG-3Y1y_ilt9EVqea55bK0NpOa5CBQ4slmOCa9MaB7CIC-ALbfGGPbfpZMCFNcsxeNjHrDgLPDIwYZx00Y7LVLE-A-YjDg0P2ubQa_gm1OFLVeLFI-rI3A-Q-GRBzWft7OB2ZwwKXsekVsSsLWc7ZZicuqwy3mQyDjiHjncyMrvZV1Ir8NWmC6nbegk5LJ-6WA0GrW_Hv3DDI_JDbyuwwwPSGc5X9knYJcus6eN1vkFm2CKXA
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Efficient+transduction+and+optogenetic+stimulation+of+retinal+bipolar+cells+by+a+synthetic+adeno%E2%80%90associated+virus+capsid+and+promoter&rft.jtitle=EMBO+molecular+medicine&rft.au=Cronin%2C+Therese&rft.au=Vandenberghe%2C+Luk+H&rft.au=Hantz%2C+P%C3%A9ter&rft.au=Juttner%2C+Josephine&rft.date=2014-09-01&rft.issn=1757-4676&rft.eissn=1757-4684&rft.volume=6&rft.issue=9&rft.spage=1175&rft.epage=1190&rft_id=info:doi/10.15252%2Femmm.201404077&rft.externalDBID=n%2Fa&rft.externalDocID=10_15252_emmm_201404077
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1757-4676&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1757-4676&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1757-4676&client=summon