Probiotics and colostrum/milk differentially affect neonatal humoral immune responses to oral rotavirus vaccine

• Published in: Vaccine Vol. 31; no. 15; pp. 1916 - 1923
• Main Authors: Chattha, Kuldeep S., Vlasova, Anastasia N., Kandasamy, Sukumar, Esseili, Malak A., Siegismund, Christine, Rajashekara, Gireesh, Saif, Linda J.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 08.04.2013; Elsevier; Elsevier Limited
• Subjects: Administration, Oral; Age; Allergy and Immunology; Anatomy & physiology; Animals; Animals, Newborn - immunology; Animals, Newborn - microbiology; Antibody responses; Applied microbiology; B-lymphocytes; B-Lymphocytes - immunology; bacteria; Bifidobacterium - immunology; Bifidobacterium - physiology; Bifidobacterium animalis; Bifidobacterium lactis; Biological and medical sciences; blood serum; breast milk; Colonization; Colony Count, Microbial; colostrum; Colostrum - chemistry; Colostrum - immunology; Colostrum/milk; Cytokines; digestive system; feces; Feces - microbiology; Female; Fundamental and applied biological sciences. Psychology; Germ-Free Life; Hogs; homeostasis; Human rotavirus; Humans; humoral immunity; immune response; Immunity, Humoral - immunology; immunoglobulin A; Immunoglobulin A - analysis; Immunoglobulin A - immunology; immunoglobulin G; Immunoglobulin G - analysis; Immunoglobulin G - immunology; infant formulas; Intestines - immunology; Intestines - microbiology; Lactobacillus rhamnosus; Lactobacillus rhamnosus - immunology; Lactobacillus rhamnosus - physiology; Microbiology; Milk; Milk, Human - chemistry; Milk, Human - immunology; Miscellaneous; Models, Animal; pathogens; Probiotics; Probiotics (LGG + Bb12); Probiotics - administration & dosage; Probiotics - pharmacology; Rotavirus; Rotavirus - immunology; Rotavirus Infections - immunology; Rotavirus Infections - prevention & control; Rotavirus vaccine; Rotavirus Vaccines - administration & dosage; Rotavirus Vaccines - immunology; Studies; Sus scrofa - immunology; Sus scrofa - microbiology; Swine; swine feeding; TGFβ; Transforming Growth Factor beta - blood; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - immunology; Virology; Viruses; TGFβ; Antibody responses; Rotavirus vaccine; Probiotics (LGG+Bb12); Colonization; Colostrum/milk; Probiotics (LGG + Bb12); Probiotic; Immune response; Rotavirus; Vaccine; Reoviridae; Virus; Humoral immunity; Milk
• ISSN: 0264-410X; 1873-2518; 1873-2518
• DOI: 10.1016/j.vaccine.2013.02.020

► Col/milk supplementation enhanced probiotic fecal shedding in unvaccinated animals. ► Col/milk fed pigs had suppressed IgA HRV antibody responses after 2nd vaccine dose. ► Probiotics enhanced IgA HRV responses in Pro+Vac+Col/milk pigs after 3rd vaccine dose. ► Probiotic colonization in Vac+Pro pigs reduced IgG HRV antibody responses. Breast milk (colostrum [col]/milk) components and gut commensals play important roles in neonatal immune maturation, establishment of gut homeostasis and immune responses to enteric pathogens and oral vaccines. We investigated the impact of colonization by probiotics, Lactobacillus rhamnosus GG (LGG) and Bifidobacterium lactis Bb12 (Bb12) with/without col/milk (mimicking breast/formula fed infants) on B lymphocyte responses to an attenuated (Att) human rotavirus (HRV) Wa strain vaccine in a neonatal gnotobiotic pig model. Col/milk did not affect probiotic colonization in AttHRV vaccinated pigs. However, unvaccinated pigs fed col/milk shed higher numbers of probiotic bacteria in feces than non-col/milk fed colonized controls. In AttHRV vaccinated pigs, col/milk feeding with probiotic treatment resulted in higher mean serum IgA HRV antibody titers and intestinal IgA antibody secreting cell (ASC) numbers compared to col/milk fed, non-colonized vaccinated pigs. In vaccinated pigs without col/milk, probiotic colonization did not affect IgA HRV antibody titers, but serum IgG HRV antibody titers and gut IgG ASC numbers were lower, suggesting that certain probiotics differentially impact HRV vaccine responses. Our findings suggest that col/milk components (soluble mediators) affect initial probiotic colonization, and together, they modulate neonatal antibody responses to oral AttHRV vaccine in complex ways.