Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells - Levin, Gabriel; Koga, Bruna Andrade Aguiar; Belchior, Gustavo Gross; Carreira, Ana Claudia Oliveira; Sogayar, Mari Cleide (BMC biotechnology, 2020)
Keywords: Affinity chromatography; Analysis; Anticoagulants; Applied Microbiology; Beta cells; bioactive properties; Biochemical Engineering; Biological activity; Biomedical Engineering/Biotechnology; Biotechnology; blood serum; bone formation; Bone growth; Cell culture; Cell death; Cell differentiation; Cell proliferation; Cell therapy; Chains; Chemistry; Chemistry and Materials Science; Chromatography; clones; Cloning; Coding; Galactose; gel chromatography; Genetic Engineering; Genetic engineering in animals and animal cell systems; genetic vectors; Glycan; Glycosylation; HEK293 cells; Heparin; Heterologous mammalian protein expression system; Human cell culture; human cell lines; humans; Hygromycin; Identification and classification; Intestine; intestines; islets of Langerhans; Mass spectrometry; Mass spectroscopy; medicine; Methods; mice; Muscles; N-Acetylglucosamine; neoplasms; Oligosaccharides; Osteogenesis; Pancreas; Peptide growth factors; Plant Breeding/Biotechnology; plasmids; Polypeptides; Production processes; Protein purification; protein secretion; Proteins; Purification; Recombinant human R-spondin1 (RSPO1); Recombinant proteins; Regenerative medicine; Research Article; sialic acid; Signal transduction; Skeletal muscle; Stem cell transplantation; Stem cells; Structural integrity; therapeutics; Tissue engineering; Western blotting; Brazil; Heterologous mammalian protein expression system; HEK293 cells; Tissue engineering; Peptide growth factors; Recombinant human R-spondin1 (RSPO1)