Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: a cross sectional study

• Published in: BMC infectious diseases Vol. 11; no. 1; p. 222
• Main Authors: Addis, Zelalem, Kebede, Nigatu, Sisay, Zufan, Alemayehu, Haile, Wubetie, Alehegne, Kassa, Tesfu
• Format: Journal Article
• Language: English
• Published: London BioMed Central 19.08.2011; BioMed Central Ltd; BMC
• Subjects: Agriculture; Animals; Anti-Bacterial Agents - pharmacology; Anti-infective agents; Antimicrobial agents; Bacteria; Bacterial and fungal diseases; Bacteriological Techniques - methods; Care and treatment; Carrier State - epidemiology; Carrier State - microbiology; Carrier State - veterinary; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Causes of; Cross-Sectional Studies; Dairy farms; Demographic aspects; Dosage and administration; Drug resistance in microorganisms; Drug Resistance, Bacterial; Ethiopia - epidemiology; Feces - microbiology; Food; Health services; Human subjects; Humans; Infectious Diseases; Internal Medicine; International organizations; Medical Microbiology; Medicine; Medicine & Public Health; Milk - microbiology; Parasitology; Prevalence; Prevention; Research Article; Risk factors; Salmonella - drug effects; Salmonella - isolation & purification; Salmonella Infections - epidemiology; Salmonella Infections - microbiology; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; Salmonellosis; Sample size; Statistical analysis; Syntax; Tropical Medicine; Ethiopia; Addis Ababa Ethiopia; Dairy Farm; Important Public Health Problem; Antimicrobial Resistance; Cheddar Cheese; Milk Sample
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/1471-2334-11-222

Background Salmonella are the major pathogenic bacteria in humans as well as in animals. Salmonella species are leading causes of acute gastroenteritis in several countries and salmonellosis remains an important public health problem worldwide, particularly in the developing countries. The situation is more aggravated by the ever increasing rate of antimicrobial resistance strains. Cattle have been implicated as a source of human infection with antimicrobial resistant Salmonella through direct contact with livestock and through the isolation of antimicrobial resistant Salmonella from raw milk, cheddar cheese, and hamburger meat traced to dairy farms. Despiite the presence of many studies on the prevalence and antimicrobial susceptibility pattern of Salmonella in Ethiopia, nothing has been said on the degree of the situation among apparently healthy lactating cows and in contact humans. Hence this study was conducted to determine the prevalence and antimicrobial resistance pattern of Salmonella isolates from lactating cows and in contact humans in dairy farms of Addis Ababa. Methods a cross sectional study was conducted in Addis Ababa by collecting milk and faecal samples from lactating cows and stool samples from humans working in dairy farms. Samples were pre-enriched in buffered peptone water followed by selective enrichment using selenite cysteine and Rapaport-Vassilidis broths. Isolation and identification was made by inoculating the selectively enriched sample on to Xylose Lysine Deoxycholate agar followed by confirmation of presumptive colonies using different biochemical tests. The Kibry Bauer disk diffusion method was used for antimicrobial sensitivity testing. Results 10.7% (21/195) of cows and 13.6% (3/22) of the human subjects sheded Salmonella . 83% resistance to two or more antimicrobials and 100% resistance to ampicillin were observed. Most of the isolates were relatively sensitive to ciprofloxacin, cotrimoxazole, and chloramphenicol. Conclusion High proportion of Salmonella isolates developed resistance to the commonly prescribed antimicrobials and this may be a considerable risk in the treatment of clinical cases. So, wise use of antimicrobials must be practiced to combat the ever increasing situation of antimicrobial resistance.